Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine

PAMUKÇU, ESRA; ERÖKSÜZ, Yesari; KANDEMİR, Fatih Mehmet; ÖZDEMİR, Necmi; KAYMAZ, Mustafa Bahadır

Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine

Mustafa Bahadır KAYMAZ, (Department of Pharmacology, Faculty of Pharmacy, University of Inonu, TR-44280 Malatya - TURKEY)

Fatih Mehmet KANDEMİR, (Department of Biochemistry, Faculty of Veterinary Medicine, University of Ataturk, TR-25240 Erzurum - TURKEY)

Esra PAMUKÇU, (Department of Statistics, Faculty of Science, University of Firat, TR-23200, Elazig - TURKEY)

Yesari ERÖKSÜZ, (Department of Pathology, Faculty of Veterinary Medicine, University of Firat, TR-23200 Elazig - TURKEY)

Necmi ÖZDEMİR (Department of Biochemistry, Faculty of Veterinary Medicine, University of Firat, TR-23200 Elazig - TURKEY)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

10 1

Yıl: 2017 Cilt: 23 Sayı: 1 Sayfa Aralığı: 155 - 160 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine

Öz:

Dünyada mantar zehirlenmelerine bağlı ölümlerin yaklaşık 90%'ına Amanita phalloides türü mantarlar neden olmaktadır. Alfa-amanitin, bisiklik oktapeptid yapıda bu zehirlenmelerden sorumlu ana yapıdır. Bu çalışmada, enginar sulu yaprak ekstresinin oluşturulan toksisite üzerine etkilerinin araştırılması amaçlanmıştır. Çalışmada, Sprague-Dawley cinsi 28 erkek rat rastgele 4 gruba ayrıldı. Gruplar, kontrol grubu (serum fizyolojik 0.1 mL ip), alfa-amanitin (3 mg/kg tek doz ip), enginar yaprak ekstresi (oral 1.5 g/kg, 14 gün) ve tedavi uygulanan (alfaamanitin 3 mg/kg tek doz ip+ enginar yaprak ekstresi oral 1.5 g/kg, 14 gün) olmak üzere belirlendi. Alfa-amanitinin karaciğer malondialdehit (MDA) düzeyini arttırdığı, süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px), katalaz (CAT) aktiviteleri ile glutatyon (GSH) düzeyini düşürdüğü tespit edilmiştir. Enginar sulu yaprak ekstresi takviyesinin MDA seviyesini düşürürken antioksidan parametrelerde ve histopatolojik bulgularda iyileşmeye neden olduğu saptanmıştır. Çalışma sonucunda, alfa-amanitin kaynaklı karaciğer hasarına karşı enginarın sulu ekstresinin kullanımının, tedaviye destek olarak kullanılabileceği düşünülmektedir.

Anahtar Kelime:

Konular: Veterinerlik

Alfa-Amanitin İle Oluşturulmuş Karaciğer Hasari Üzerine Enginar (Cynara scolymus) Sulu Yaprak Ekstresinin Etkileri

Öz:

Approximately 90% of mushroom poisoning deaths in the world is caused by types of Amanita phalloides. Alpha-amanitine has a bicyclic octapeptide structure, which is the main structure responsible for these poisoning cases. In the present study, it was aimed to investigate effects of leaf extracts with artichoke extract on this toxicity. In the study, 28 male rats of Sprague-Dawley species were randomized to 4 groups. The groups were designed as control; receiving serum physiological solution of 0.1 mL intraperitoneally (ip), alpha-amanitine; receiving 3 mg/kg single dose ip, artichoke leaf extract; receiving 1.5 g/kg orally for 14 d, and treatment group¸ receiving alpha-amanitine 3 mg/kg single dose ip+artichoke leaf extract 1.5 g/kg orally for 14 d. It was determined that alpha-amanitine increased hepatic malondialdehyde (MDA) levels, and decrease superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activities with decreasing glutathione (GSH) levels. The supplementation with extract with artichoke, decreased MDA levels, it improved antioxidant parameters, and histopathological findings, so it was decided that extract with artichoke juice might be beneficial in alpha-amanitine related hepatic damage.

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KAYMAZ M, KANDEMİR F, PAMUKÇU E, ERÖKSÜZ Y, ÖZDEMİR N (2017). Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine. , 155 - 160.
Chicago	KAYMAZ Mustafa Bahadır,KANDEMİR Fatih Mehmet,PAMUKÇU ESRA,ERÖKSÜZ Yesari,ÖZDEMİR Necmi Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine. (2017): 155 - 160.
MLA	KAYMAZ Mustafa Bahadır,KANDEMİR Fatih Mehmet,PAMUKÇU ESRA,ERÖKSÜZ Yesari,ÖZDEMİR Necmi Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine. , 2017, ss.155 - 160.
AMA	KAYMAZ M,KANDEMİR F,PAMUKÇU E,ERÖKSÜZ Y,ÖZDEMİR N Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine. . 2017; 155 - 160.
Vancouver	KAYMAZ M,KANDEMİR F,PAMUKÇU E,ERÖKSÜZ Y,ÖZDEMİR N Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine. . 2017; 155 - 160.
IEEE	KAYMAZ M,KANDEMİR F,PAMUKÇU E,ERÖKSÜZ Y,ÖZDEMİR N "Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine." , ss.155 - 160, 2017.
ISNAD	KAYMAZ, Mustafa Bahadır vd. "Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine". (2017), 155-160.

APA	KAYMAZ M, KANDEMİR F, PAMUKÇU E, ERÖKSÜZ Y, ÖZDEMİR N (2017). Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23(1), 155 - 160.
Chicago	KAYMAZ Mustafa Bahadır,KANDEMİR Fatih Mehmet,PAMUKÇU ESRA,ERÖKSÜZ Yesari,ÖZDEMİR Necmi Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 23, no.1 (2017): 155 - 160.
MLA	KAYMAZ Mustafa Bahadır,KANDEMİR Fatih Mehmet,PAMUKÇU ESRA,ERÖKSÜZ Yesari,ÖZDEMİR Necmi Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.23, no.1, 2017, ss.155 - 160.
AMA	KAYMAZ M,KANDEMİR F,PAMUKÇU E,ERÖKSÜZ Y,ÖZDEMİR N Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2017; 23(1): 155 - 160.
Vancouver	KAYMAZ M,KANDEMİR F,PAMUKÇU E,ERÖKSÜZ Y,ÖZDEMİR N Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2017; 23(1): 155 - 160.
IEEE	KAYMAZ M,KANDEMİR F,PAMUKÇU E,ERÖKSÜZ Y,ÖZDEMİR N "Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23, ss.155 - 160, 2017.
ISNAD	KAYMAZ, Mustafa Bahadır vd. "Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 23/1 (2017), 155-160.