Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain

DEMİRKAN, Elif; AKCAKOCA, Dilara; Ersoy, Figen; SEVGİ, Tuba

Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain

Elif DEMİRKAN, (Uludag University,)

Tuba SEVGİ, (Uludag University,)

Dilara AKCAKOCA, (Uludag University,)

Figen ERSOY (Uludag University,)

Türk Biyokimya Dergisi

10 1

Yıl: 2017 Cilt: 42 Sayı: 3 Sayfa Aralığı: 329 - 337 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain

Öz:

Amaç: Bu çalışma, topraktan izole edilmiş bir Bacillus sp. suşundan yeni fitaz enziminin karakterizasyonunu ve bakteriyel adlandırmayı rapor etmek için tasarlanmıştır.Metod: Bacillus sp. suşu 16S rRNA analizin ile tanımlanmıştır. Fitaz bir dizi basamak ile saflaştırılmış, aktivite ve stabilite için karakterize edilmiştir.Bulgular: Yeni izolat EBD 9-1 Bacillus megaterium ile % 100 sekans benzerliği göstermiştir. Kısmen saflaştırılmış enzim, pH 7.0 ve 60°C maksimum aktiviteye sahipti. Enzim aktivitesi Ca + 2 varlığında uyarılmıştır. Enzim için Vmax ve Kmsırasıyla, 333 U/mL ve 2 mM olarak bulunmuştur. Kısmen saflaştırılmış enzimin tahmini molekül ağırlığı 45 kDa'du. Fitaz depolama stabilitesi 4°C'de ve - 20°C'de altı aydan sonra başlangıç aktivitesinin % 93?di. Bu çalışma B. megaterium fitaz için kısmen saflaştırma, karakterizasyonu ve buğday kepeğini parçalanmasını göstermektedir.Sonuç: Sonuç olarak, Bacillus megaterium EBD 9-1 tarafından üretilen yeni bir fitaz enziminin daha yüksek sıcaklıklarda, alkalin pH'da stabil olması ve depolama özellikleri nedeniyle, enzim fitatlardan fosforun kullanılabilirliğini artırmak için hayvan yemlerine ilave için uygun olabilir

Anahtar Kelime:

Konular: Biyoloji Biyokimya ve Moleküler Biyoloji

Bacillus megaterium EBD 9-1 suşundan yeni bir fitazın kısmen saflaştırılması, karakterizasyonu ve buğday kepeğini parçalama çalışmaları

Öz:

Objective: The present study was designed to report the bacterial identification and characterization of a new phytase enzyme from a Bacillus sp. strain isolated from soil. Methods: Bacillus sp. strain was identified based on 16S rRNA analysis. The phytase was partially purified through ammonium sulfate precipitation and Sephadex G100 gel filtration steps, and characterized for its activity and stability.Results: The new isolate EBD 9-1 showed 100% sequence identity with Bacillus megaterium. The partially purified enzyme had the maximum activity at pH 7.0 and 60°C. The activity of the enzyme was stimulated in the presence of Ca + 2, Vmax and Km for enzyme were found to be 333 U/mL and 2 mM, respectively. The estimated molecular weight of enzyme was 45 kDa. The storage stability of phytase was 93% of the initial activity after 6 months at 4°C and − 20°C. This study represents the partial purification

Anahtar Kelime:

Konular: Biyoloji Biyokimya ve Moleküler Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	DEMİRKAN E, SEVGİ T, AKCAKOCA D, Ersoy F (2017). Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain. , 329 - 337.
Chicago	DEMİRKAN Elif,SEVGİ Tuba,AKCAKOCA Dilara,Ersoy Figen Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain. (2017): 329 - 337.
MLA	DEMİRKAN Elif,SEVGİ Tuba,AKCAKOCA Dilara,Ersoy Figen Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain. , 2017, ss.329 - 337.
AMA	DEMİRKAN E,SEVGİ T,AKCAKOCA D,Ersoy F Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain. . 2017; 329 - 337.
Vancouver	DEMİRKAN E,SEVGİ T,AKCAKOCA D,Ersoy F Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain. . 2017; 329 - 337.
IEEE	DEMİRKAN E,SEVGİ T,AKCAKOCA D,Ersoy F "Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain." , ss.329 - 337, 2017.
ISNAD	DEMİRKAN, Elif vd. "Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain". (2017), 329-337.

APA	DEMİRKAN E, SEVGİ T, AKCAKOCA D, Ersoy F (2017). Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain. Türk Biyokimya Dergisi, 42(3), 329 - 337.
Chicago	DEMİRKAN Elif,SEVGİ Tuba,AKCAKOCA Dilara,Ersoy Figen Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain. Türk Biyokimya Dergisi 42, no.3 (2017): 329 - 337.
MLA	DEMİRKAN Elif,SEVGİ Tuba,AKCAKOCA Dilara,Ersoy Figen Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain. Türk Biyokimya Dergisi, vol.42, no.3, 2017, ss.329 - 337.
AMA	DEMİRKAN E,SEVGİ T,AKCAKOCA D,Ersoy F Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain. Türk Biyokimya Dergisi. 2017; 42(3): 329 - 337.
Vancouver	DEMİRKAN E,SEVGİ T,AKCAKOCA D,Ersoy F Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain. Türk Biyokimya Dergisi. 2017; 42(3): 329 - 337.
IEEE	DEMİRKAN E,SEVGİ T,AKCAKOCA D,Ersoy F "Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain." Türk Biyokimya Dergisi, 42, ss.329 - 337, 2017.
ISNAD	DEMİRKAN, Elif vd. "Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain". Türk Biyokimya Dergisi 42/3 (2017), 329-337.