Yıl: 2017 Cilt: 41 Sayı: 6 Sayfa Aralığı: 919 - 925 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts

Öz:
The MTT assay is one of the often used cell viability/cytotoxicity assays. However, when the methanol extracts of plantsare used to test their cytotoxic potential, interference may occur, resulting in false-positive viability results. Therefore, in this study,the reliability of the MTT assay was investigated in the case of plant use. The methanol extracts of three different plants ( Hypericumadenotrichum ,Salvia kronenburgii , andPelargonium quercetorum ) were tested in breast cancer cell lines (MCF-7 and MDA-MB-231)using the MTT assay and the results were compared to the ATP assay, which is a much more sensitive and reliable assay due to itsinterference-free feature. Additionally, decreased cell density was confirmed with phase-contrast microscopy and fluorescence staining(Hoechst 33342 dye). Although both of the viability/cytotoxicity assays are considered as metabolic assays, viabilities (in %) in the MTTassay were found to be strikingly higher when compared to the results with the ATP assay. Even in the case of total death, the MTTassay still produced artificial/false increases in viability. The morphology-based evaluation of viability/cytotoxicity by phase-contrastmicroscopy and Hoechst 33342 staining were greatly compatible with the ATP assay results. Overestimated (false) viabilities in the MTTassay suggests a serious interference between the MTT assay itself and the extracts used. Some ingredients of plants may have reducingactivity (like the dehydrogenase activity of the cells) that converts the MTT compound into the colored formazan that is the principleof the assay. Therefore, the MTT assay may not be a suitable assay for some plant extracts, urging great caution when plants are used.
Anahtar Kelime:

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA DKARAKAŞ İ, Ari F, ULUKAYA E (2017). The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts. , 919 - 925.
Chicago DKARAKAŞ İdem,Ari Ferda,ULUKAYA Engin The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts. (2017): 919 - 925.
MLA DKARAKAŞ İdem,Ari Ferda,ULUKAYA Engin The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts. , 2017, ss.919 - 925.
AMA DKARAKAŞ İ,Ari F,ULUKAYA E The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts. . 2017; 919 - 925.
Vancouver DKARAKAŞ İ,Ari F,ULUKAYA E The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts. . 2017; 919 - 925.
IEEE DKARAKAŞ İ,Ari F,ULUKAYA E "The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts." , ss.919 - 925, 2017.
ISNAD DKARAKAŞ, İdem vd. "The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts". (2017), 919-925.
APA DKARAKAŞ İ, Ari F, ULUKAYA E (2017). The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts. Turkish Journal of Biology, 41(6), 919 - 925.
Chicago DKARAKAŞ İdem,Ari Ferda,ULUKAYA Engin The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts. Turkish Journal of Biology 41, no.6 (2017): 919 - 925.
MLA DKARAKAŞ İdem,Ari Ferda,ULUKAYA Engin The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts. Turkish Journal of Biology, vol.41, no.6, 2017, ss.919 - 925.
AMA DKARAKAŞ İ,Ari F,ULUKAYA E The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts. Turkish Journal of Biology. 2017; 41(6): 919 - 925.
Vancouver DKARAKAŞ İ,Ari F,ULUKAYA E The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts. Turkish Journal of Biology. 2017; 41(6): 919 - 925.
IEEE DKARAKAŞ İ,Ari F,ULUKAYA E "The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts." Turkish Journal of Biology, 41, ss.919 - 925, 2017.
ISNAD DKARAKAŞ, İdem vd. "The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts". Turkish Journal of Biology 41/6 (2017), 919-925.