Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage)

GÜLBAZ, GÜVEN; AKÇA, ATILA; ARSLAN, MÜKREMİN ÖZKAN; TAŞÇI, Gencay Taşkın; KAMBER, Ufuk

Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage)

Ufuk KAMBER, (Kafkas Üniversitesi, Veteriner Fakültesi, Kars, Türkiye)

Mükremin Özkan ARSLAN, (Kafkas Üniversitesi, Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Kars, Türkiye)

Güven GÜLBAZ, (Kafkas Üniversitesi, Mühendislik Mimarlık Fakültesi, Gıda Mühendisliği Bölümü, Kars, Türkiye)

Gencay Taşkın TAŞÇI, (Kafkas Üniversitesi, Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Kars, Türkiye)

Atila AKÇA (Kafkas Üniversitesi, Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Kars, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

1 0

Yıl: 2018 Cilt: 42 Sayı: 1 Sayfa Aralığı: 1 - 6 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage)

Öz:

Sarcocystisspecies are the most commonly encountered parasitic organisms in animals.Sarcocystis hominis( Sarcocystisbovihominis ), the species found in beef cattle, is not visible macroscopically in muscle tissue. In recent years it has been establishedthat, in addition to being a zoonotic parasite transmitted via the consumption of beef, the parasite produces toxins and causes foodpoisoning. Therefore, this study was undertaken in order to determine the extent to whichSarcocystisspecies are present in mincedmeat, meatballs, and fermented sausages produced from beef and marketed in Kars. A total of 150 samples, 50 of each product, werecollected in the city center from butcher s shops, from businesses that produce such items, and from general stores. In order to detectsarcocysts in the samples, the cysts revealed by an enzymatic digestion test were examined microscopically. Genomic DNA extractedfrom the samples was stored at20 °C for analysis by polymerase chain reaction (PCR). The microscopic analysis revealed that theparasite was present in 2 samples of minced meat (4%) and 1 sample of meatballs (2%). Analysis by PCR revealed that the parasite waspresent in 14 samples of minced meat (28%), 34 samples of meatballs (68%), and 1 sample of sausage (2%). These results indicate that theuse of PCR in the routine analysis of meat preparations for the presence ofSarcocystisspecies should be increased, since the applicationof this technique would provide analysts with more conclusive results than examination by microscope.

Anahtar Kelime:

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KAMBER U, ARSLAN M, GÜLBAZ G, TAŞÇI G, AKÇA A (2018). Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage). , 1 - 6.
Chicago	KAMBER Ufuk,ARSLAN MÜKREMİN ÖZKAN,GÜLBAZ GÜVEN,TAŞÇI Gencay Taşkın,AKÇA ATILA Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage). (2018): 1 - 6.
MLA	KAMBER Ufuk,ARSLAN MÜKREMİN ÖZKAN,GÜLBAZ GÜVEN,TAŞÇI Gencay Taşkın,AKÇA ATILA Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage). , 2018, ss.1 - 6.
AMA	KAMBER U,ARSLAN M,GÜLBAZ G,TAŞÇI G,AKÇA A Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage). . 2018; 1 - 6.
Vancouver	KAMBER U,ARSLAN M,GÜLBAZ G,TAŞÇI G,AKÇA A Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage). . 2018; 1 - 6.
IEEE	KAMBER U,ARSLAN M,GÜLBAZ G,TAŞÇI G,AKÇA A "Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage)." , ss.1 - 6, 2018.
ISNAD	KAMBER, Ufuk vd. "Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage)". (2018), 1-6.

APA	KAMBER U, ARSLAN M, GÜLBAZ G, TAŞÇI G, AKÇA A (2018). Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage). Turkish Journal of Veterinary and Animal Sciences, 42(1), 1 - 6.
Chicago	KAMBER Ufuk,ARSLAN MÜKREMİN ÖZKAN,GÜLBAZ GÜVEN,TAŞÇI Gencay Taşkın,AKÇA ATILA Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage). Turkish Journal of Veterinary and Animal Sciences 42, no.1 (2018): 1 - 6.
MLA	KAMBER Ufuk,ARSLAN MÜKREMİN ÖZKAN,GÜLBAZ GÜVEN,TAŞÇI Gencay Taşkın,AKÇA ATILA Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage). Turkish Journal of Veterinary and Animal Sciences, vol.42, no.1, 2018, ss.1 - 6.
AMA	KAMBER U,ARSLAN M,GÜLBAZ G,TAŞÇI G,AKÇA A Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage). Turkish Journal of Veterinary and Animal Sciences. 2018; 42(1): 1 - 6.
Vancouver	KAMBER U,ARSLAN M,GÜLBAZ G,TAŞÇI G,AKÇA A Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage). Turkish Journal of Veterinary and Animal Sciences. 2018; 42(1): 1 - 6.
IEEE	KAMBER U,ARSLAN M,GÜLBAZ G,TAŞÇI G,AKÇA A "Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage)." Turkish Journal of Veterinary and Animal Sciences, 42, ss.1 - 6, 2018.
ISNAD	KAMBER, Ufuk vd. "Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage)". Turkish Journal of Veterinary and Animal Sciences 42/1 (2018), 1-6.