Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium

ENGİN, SEÇKİN; BARUT, ELİF NUR; YILDIRIM, SERCAN; TÜRKİŞ, Sevda; Barut, Burak; SEZEN, FERİDE SENA; Özel, Arzu; YAŞAR, AHMET

Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium

Elif Nur BARUT, (Boş)

Burak BARUT, (Boş)

Seçkin ENGİN, (Boş)

Sercan YILDIRIM, (Boş)

Ahmet YAŞAR, (Boş)

Sevda TÜRKİŞ, (Boş)

Arzu ÖZEL, (Boş)

Feride Sena SEZEN (Boş)

Türk Biyokimya Dergisi

3 0

Yıl: 2017 Cilt: 42 Sayı: 4 Sayfa Aralığı: 493 - 502 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium

Öz:

Amaç: Achillea millefolium (A. millefolium), antioksidan aktiviteye sahip doğal bileşikler içeren ve halk arasında çeşitli amaçlar için kullanılan geleneksel tıbbi bir bitkidir Çalışmamızın amacı, A. millefolium ekstrelerinin Alzheimer hastalığının (AD) tedavisinde kullanılma potansiyelini ortaya koymak için gerekli antioksidan ve anti-asetilkolinesteraz aktivitelerini araştırmaktır.Metod: A. millefolium'un metanol (ME) ve etanol ekstraktları (EE) hazırlanarak, (a) in vitro antioksidan aktiviteleri, (b) Asetilkolinesteraz kinetiği üzerindeki etkileri (kolorimetrik spektroskopik yöntem kullanılarak) ve (c) Fare beyin homojenatında sodyum nitroprusiyat (SNP) ile indüklenen lipid peroksidasyona etkisi incelenmiştir.Bulgular: ME, EE'den daha yüksek antioksidan aktiviteye sahiptir. Her iki ekstrakt da yarışmalı asetilkolinesteraz inhibisyonuna neden olmaktadır; ancak ME'nin bu inhibitor etkisi EE'den daha yüksektir. ME ve EE'nin inhibitor sabitleri (Ki) sırasıyla 28.43 ve 68.47 ?g/mL olarak bulunmuştur. Her iki ekstrakt da fare beyin homojenatındaki malondialdehit (MDA) düzeylerinde konsantrasyona bağlı olarak anlamlı bir azalmaya neden olmuş ve bu durum güçlü bir lipid peroksidasyonunun inhibisyonunu göstermektedir.Sonuç: Bu sonuçlar A. millefolium'un sahip olduğu yüksek antioksidan kapasite ve anti-asetilkolinesteraz aktivitesinin taşıdığı fenolik bileşiklerden kaynaklandığı ve bu sebeple AD gibi nörodejeneratif hastalıklarda destekleyici tedavi yaklaşımında doğal terapötik bir ajan olarak kullanılabilirliği gösterilmiştir

Anahtar Kelime:

Konular: Fizik, Uygulamalı Biyokimya ve Moleküler Biyoloji

Alchillea millefolium’un antioksidan kapasitesi, antiasetilkolinesteraz aktivitesi ve fare beyin homojenatında lipid peroksidasyonu üzerine inhibitör etkisi

Öz:

Objective: Achillea millefolium (A. millefolium) is a traditional herbal medicine that contains natural compounds with antioxidant activities and is used for a wide range of conditions among people. The aim of our study was to investigate antioxidant and anti-acetylcholinesterase activities of A. millefolium extracts to determine its potential therapeutic usage in Alzheimer’s disease (AD).Methods: Methanol extracts (ME) and ethanol extracts (EE) of A. millefolium were prepared to determine (a) in vitro antioxidant activities, (b) the effects on acetylcholinesterase kinetics (by using a colorimetric spectroscopic method) and (c) the effects on sodium nitroprusside (SNP)-induced lipid peroxidation in mice brain homogenate

Anahtar Kelime:

Konular: Fizik, Uygulamalı Biyokimya ve Moleküler Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	BARUT E, Barut B, ENGİN S, YILDIRIM S, YAŞAR A, TÜRKİŞ S, Özel A, SEZEN F (2017). Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium. , 493 - 502.
Chicago	BARUT ELİF NUR,Barut Burak,ENGİN SEÇKİN,YILDIRIM SERCAN,YAŞAR AHMET,TÜRKİŞ Sevda,Özel Arzu,SEZEN FERİDE SENA Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium. (2017): 493 - 502.
MLA	BARUT ELİF NUR,Barut Burak,ENGİN SEÇKİN,YILDIRIM SERCAN,YAŞAR AHMET,TÜRKİŞ Sevda,Özel Arzu,SEZEN FERİDE SENA Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium. , 2017, ss.493 - 502.
AMA	BARUT E,Barut B,ENGİN S,YILDIRIM S,YAŞAR A,TÜRKİŞ S,Özel A,SEZEN F Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium. . 2017; 493 - 502.
Vancouver	BARUT E,Barut B,ENGİN S,YILDIRIM S,YAŞAR A,TÜRKİŞ S,Özel A,SEZEN F Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium. . 2017; 493 - 502.
IEEE	BARUT E,Barut B,ENGİN S,YILDIRIM S,YAŞAR A,TÜRKİŞ S,Özel A,SEZEN F "Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium." , ss.493 - 502, 2017.
ISNAD	BARUT, ELİF NUR vd. "Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium". (2017), 493-502.

APA	BARUT E, Barut B, ENGİN S, YILDIRIM S, YAŞAR A, TÜRKİŞ S, Özel A, SEZEN F (2017). Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium. Türk Biyokimya Dergisi, 42(4), 493 - 502.
Chicago	BARUT ELİF NUR,Barut Burak,ENGİN SEÇKİN,YILDIRIM SERCAN,YAŞAR AHMET,TÜRKİŞ Sevda,Özel Arzu,SEZEN FERİDE SENA Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium. Türk Biyokimya Dergisi 42, no.4 (2017): 493 - 502.
MLA	BARUT ELİF NUR,Barut Burak,ENGİN SEÇKİN,YILDIRIM SERCAN,YAŞAR AHMET,TÜRKİŞ Sevda,Özel Arzu,SEZEN FERİDE SENA Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium. Türk Biyokimya Dergisi, vol.42, no.4, 2017, ss.493 - 502.
AMA	BARUT E,Barut B,ENGİN S,YILDIRIM S,YAŞAR A,TÜRKİŞ S,Özel A,SEZEN F Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium. Türk Biyokimya Dergisi. 2017; 42(4): 493 - 502.
Vancouver	BARUT E,Barut B,ENGİN S,YILDIRIM S,YAŞAR A,TÜRKİŞ S,Özel A,SEZEN F Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium. Türk Biyokimya Dergisi. 2017; 42(4): 493 - 502.
IEEE	BARUT E,Barut B,ENGİN S,YILDIRIM S,YAŞAR A,TÜRKİŞ S,Özel A,SEZEN F "Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium." Türk Biyokimya Dergisi, 42, ss.493 - 502, 2017.
ISNAD	BARUT, ELİF NUR vd. "Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium". Türk Biyokimya Dergisi 42/4 (2017), 493-502.