Kızılcık (Cornus mas L.) meyva ekstraktının sisplatin indüklü in vitro böbrek hücre hasarına etkisinin araştırılması

koca, ilkay; Kazak, Filiz; ÇENESİZ, Sena; Sozmen, Mahmut; ALBAYRAK, Harun; YARIM, Gül Fatma; ÖZAN, EMRE; YARIM, MURAT

Kızılcık (Cornus mas L.) meyva ekstraktının sisplatin indüklü in vitro böbrek hücre hasarına etkisinin araştırılması

Gül Fatma YARIM, (Ondokuz Mayis University, Department of Biochemistry, Faculty of Veterinary Medicine, Samsun, Atakum, Turkey)

Filiz KAZAK, (k: Mustafa Kemal University, Department of Biochemistry, Faculty of Veterinary Medicine, Hatay, Antakya, Turkey)

Mahmut SÖZMEN, (Ondokuz Mayis University, Department of Pathology, Faculty of Veterinary Medicine, Samsun, Atakum, Turkey)

İlkay KOCA, (: Ondokuz Mayis University, Department of Food Engineering, Faculty of Engineering, Samsun, Atakum, Turkey)

Harun ALBAYRAK, (Ondokuz Mayis University, Department of Virology, Faculty of Veterinary Medicine, Samsun, Atakum, Turkey)

MURAT YARIM, (Boş)

Sena ÇENESİZ, (: Ondokuz Mayis University, Department of Biochemistry, Faculty of Veterinary Medicine, Samsun, Atakum, Turkey)

EMRE ÖZAN (Veterinary Control Institute, Samsun, Atakum, Turkey)

Türk Biyokimya Dergisi

7 0

Yıl: 2017 Cilt: 42 Sayı: 4 Sayfa Aralığı: 435 - 443 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Kızılcık (Cornus mas L.) meyva ekstraktının sisplatin indüklü in vitro böbrek hücre hasarına etkisinin araştırılması

Öz:

Amaç: Bu in vitro çalışmada, kızılcık meyva ekstraktının sisplatin indüklü nefrotoksisiteye karşı koruyucu etkisinin değerlendirilmesi amaçlanmıştır.Materyal ve Metot: Çalışmada Afrika yeşil maymun böbrek epitel hücreleri (Vero), 100 mg/mL kızılcık meyva ekstraktı, 50 ?mol/L sisplatin veya 50 ?mol/L sisplatin + 100 mg/mL kızılcık meyva ekstraktı ile 4 saat inkübe edildi. Herhangi bir ekleme yapılmayan, sadece hücre bulunan kuyucuklar kontrol grubu olarak kullanıldı. Hücre canlılığı, 3-(4,5-dimetil-thiazole-2-yl)-2,5-difenil tetrazolium bromide tayini ile belirlendi. Kültür medyumları toplandı ve santrifüj edildi. Malondialdehit (MDA), glutatyon (GSH), superoksit dismutaz (SOD) ve glutatyon peroksidaz (GPx) analizleri yapıldı.Bulgular: Sisplatine maruz bırakılan hücrelerde hücre canlılılığı %42, sisplatinle birlikte kızılcık meyva ekstraktı uygulanan hücrelerde ise hücre canlılılığı %59 bulundu. Sisplatin uygulanan hücrelerde hücresel hasar oranı yüksekti. Bununla birlikte kızılcık meyva ekstraktı ile kombine sisplatin grubunda, sisplatinin zararlı etkileri anlamlı derecede azalmıştı. Sisplatin grubu, kontrol grubu, kızılcık grubu ve sisplatin + kızılcık grupları ile karşılaştırıldığında MDA konsantrasyonu önemli decede yüksek, GSH konsantrasyonu, GPx ve SOD enzim aktiviteleri anlamlı derecede düşük bulundu (p < 0.05).Sonuç: Bu çalışma sisplatin ile uyarılan in vitro oksidatif hasar üzerine kızılcık meyva ektraktının koruyucu etkileri olduğunu göstermiştir

Anahtar Kelime:

Konular: Biyoloji Hematoloji Biyokimya ve Moleküler Biyoloji

Investigation of the effect of cornelian cherry (Cornus mas L.) fruit extract against cisplatin-induced renal cell injury in vitro

Öz:

Objective: The aim of this study was to evaluate the protective effects of cornelian cherry fruit extract against cisplatin-induced nephrotoxicity in vitro.Materials and methods: African green monkey kidney epithelial cells (Vero) were incubated with 100 mg/mL of cornelian cherry fruit extract, 50 μmol/L of cisplatin or 50 μmol/L of cisplatin plus 100 mg/mL of cornelian cherry fruit extract for 4 h. The wells containing cells without any supplementation served as control. Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide assay. Culture mediums were collected, centrifuged and analyzed for malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD) and glutathione peroxidase (GPx)Results: The cell viability was 59% in cells co-treated with cisplatin and cornelian cherry fruit extract simultaneously and 42% in cisplatin treated cells. The cellular damage ratio was elevated in cells treated with cisplatin. However, when cisplatin combined with cornelian cherry fruit extract the deleterious effects of cisplatin were significantly decreased. The MDA concentration was significantly higher (p < 0.05), GSH concentration and GPx and SOD activities were significantly lower (p < 0.05) in cisplatin treated group when compared with control group, cornelian cherry group, and cisplatin + cornelian cherry group. Conclusion: The present study indicated that cornelian cherry fruit extract exert protective effects on oxidative damage in vitro induced by cisplatin.

Anahtar Kelime:

Konular: Biyoloji Hematoloji Biyokimya ve Moleküler Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	YARIM G, Kazak F, Sozmen M, koca i, ALBAYRAK H, YARIM M, ÇENESİZ S, ÖZAN E (2017). Kızılcık (Cornus mas L.) meyva ekstraktının sisplatin indüklü in vitro böbrek hücre hasarına etkisinin araştırılması. , 435 - 443.
Chicago	YARIM Gül Fatma,Kazak Filiz,Sozmen Mahmut,koca ilkay,ALBAYRAK Harun,YARIM MURAT,ÇENESİZ Sena,ÖZAN EMRE Kızılcık (Cornus mas L.) meyva ekstraktının sisplatin indüklü in vitro böbrek hücre hasarına etkisinin araştırılması. (2017): 435 - 443.
MLA	YARIM Gül Fatma,Kazak Filiz,Sozmen Mahmut,koca ilkay,ALBAYRAK Harun,YARIM MURAT,ÇENESİZ Sena,ÖZAN EMRE Kızılcık (Cornus mas L.) meyva ekstraktının sisplatin indüklü in vitro böbrek hücre hasarına etkisinin araştırılması. , 2017, ss.435 - 443.
AMA	YARIM G,Kazak F,Sozmen M,koca i,ALBAYRAK H,YARIM M,ÇENESİZ S,ÖZAN E Kızılcık (Cornus mas L.) meyva ekstraktının sisplatin indüklü in vitro böbrek hücre hasarına etkisinin araştırılması. . 2017; 435 - 443.
Vancouver	YARIM G,Kazak F,Sozmen M,koca i,ALBAYRAK H,YARIM M,ÇENESİZ S,ÖZAN E Kızılcık (Cornus mas L.) meyva ekstraktının sisplatin indüklü in vitro böbrek hücre hasarına etkisinin araştırılması. . 2017; 435 - 443.
IEEE	YARIM G,Kazak F,Sozmen M,koca i,ALBAYRAK H,YARIM M,ÇENESİZ S,ÖZAN E "Kızılcık (Cornus mas L.) meyva ekstraktının sisplatin indüklü in vitro böbrek hücre hasarına etkisinin araştırılması." , ss.435 - 443, 2017.
ISNAD	YARIM, Gül Fatma vd. "Kızılcık (Cornus mas L.) meyva ekstraktının sisplatin indüklü in vitro böbrek hücre hasarına etkisinin araştırılması". (2017), 435-443.

APA	YARIM G, Kazak F, Sozmen M, koca i, ALBAYRAK H, YARIM M, ÇENESİZ S, ÖZAN E (2017). Kızılcık (Cornus mas L.) meyva ekstraktının sisplatin indüklü in vitro böbrek hücre hasarına etkisinin araştırılması. Türk Biyokimya Dergisi, 42(4), 435 - 443.
Chicago	YARIM Gül Fatma,Kazak Filiz,Sozmen Mahmut,koca ilkay,ALBAYRAK Harun,YARIM MURAT,ÇENESİZ Sena,ÖZAN EMRE Kızılcık (Cornus mas L.) meyva ekstraktının sisplatin indüklü in vitro böbrek hücre hasarına etkisinin araştırılması. Türk Biyokimya Dergisi 42, no.4 (2017): 435 - 443.
MLA	YARIM Gül Fatma,Kazak Filiz,Sozmen Mahmut,koca ilkay,ALBAYRAK Harun,YARIM MURAT,ÇENESİZ Sena,ÖZAN EMRE Kızılcık (Cornus mas L.) meyva ekstraktının sisplatin indüklü in vitro böbrek hücre hasarına etkisinin araştırılması. Türk Biyokimya Dergisi, vol.42, no.4, 2017, ss.435 - 443.
AMA	YARIM G,Kazak F,Sozmen M,koca i,ALBAYRAK H,YARIM M,ÇENESİZ S,ÖZAN E Kızılcık (Cornus mas L.) meyva ekstraktının sisplatin indüklü in vitro böbrek hücre hasarına etkisinin araştırılması. Türk Biyokimya Dergisi. 2017; 42(4): 435 - 443.
Vancouver	YARIM G,Kazak F,Sozmen M,koca i,ALBAYRAK H,YARIM M,ÇENESİZ S,ÖZAN E Kızılcık (Cornus mas L.) meyva ekstraktının sisplatin indüklü in vitro böbrek hücre hasarına etkisinin araştırılması. Türk Biyokimya Dergisi. 2017; 42(4): 435 - 443.
IEEE	YARIM G,Kazak F,Sozmen M,koca i,ALBAYRAK H,YARIM M,ÇENESİZ S,ÖZAN E "Kızılcık (Cornus mas L.) meyva ekstraktının sisplatin indüklü in vitro böbrek hücre hasarına etkisinin araştırılması." Türk Biyokimya Dergisi, 42, ss.435 - 443, 2017.
ISNAD	YARIM, Gül Fatma vd. "Kızılcık (Cornus mas L.) meyva ekstraktının sisplatin indüklü in vitro böbrek hücre hasarına etkisinin araştırılması". Türk Biyokimya Dergisi 42/4 (2017), 435-443.