Yıl: 2017 Cilt: 48 Sayı: 2 Sayfa Aralığı: 39 - 43 Metin Dili: Türkçe

Erkek İnfertilitesinde Seminal Leptin Seviyelerinin Semen Parametreleri ve Dna Fragmantasyonu İle İlişkisi

Öz:
Amaç: Bu çalışmamızda insan semen leptin düzeylerini ve semenparametreleri ile ilişkisinin incelenmesi amaçlanmıştır.Materyal ve Metod: YNormal ve oligoastenoteratospermik 30hastalık gruplar oluşturuldu. Hastaların semen örneklerindeleptin ve DNA fragmantasyon düzeyleri incelendi, ayrıca spermsayısını ve motilite oranlarını içeren semen parametreleri de-ğerlendirildi.Bulgular: İnfertil hastalarda sperm sayısı ve motilite oranlarınormal gruba göre anlamlı olarak azalmıştır: 6,40±4,29 (x106/mL), % 38,63±14,92 (p<0,0001). DNA fragmantasyon oranlarınormal grupta % 21,00±8,23 olarak gözlenirken infertilerkeklerde % 38,43±14,23 oranıyla anlamlı olarak yüksek bulunmuş,semen leptin oraları ise normal grupta 347,58±111,13ng/mL olarak gözlenirken, infertil grupta 267,63±56,36 ng/mLdüzeyi ile anlamlı olarak azaldığı gözlenmiştir. İnfertil gruplarkendi içinde incelendiği zaman ise semen leptin düzeyi ile yaş,volüm, sperm sayısı ve motilitesi arasında istatistiksel olarakanlamlı olacak bir ilişki saptanmamıştır.Sonuç: Çalışmamızda, oligoastenoteratozoospermik erkek infertilitesindeserum leptin seviyelerinin azalmış olduğunu, amasperm motilitesi ile anlamlı bir ilikinin bulunmadı gözlemlenmiştir. Leptinin semende tanısal bir kriter olarak kullanılabilmesi için daha geni kapsamlı çalışmaların yapılması gerektiği düşünülmektedir.
Anahtar Kelime:

Konular:
Fen > Tıp > Kadın Hastalıkları ve Doğum
Fen > Tıp > Pediatri
Fen > Tıp > Cerrahi
Fen > Tıp > Genel ve Dahili Tıp

The Relationship of Seminal Leptin Levels with Semen Parameters and Dna Fragmentation

Öz:
Objective: The objective of this study is to investigate the relationship between human seminal leptin levels and semen parameters.Material and Methods: Study groups include 30 males with oligoasthenoteratozoospermia and 30 males who have normal semen parameters. Leptin and DNA fragmentation levels were investigated in both groups, along with a variety of semen parameters including sperm count and motility.Results: In comparison with the normal males, sperm count and motility have decreased significantly in infertile patients: 6,40±4,29(x106/mL), %, 38,63±14,92 (p<0,0001). DNA fragmentation levels were found as 21,00±8,23 % and 38,43±14,23 %, in normal and patient groups respectively. Seminal leptin levels were 347,58±111,13 ng/mL in the normal group, while there was a significant decrease to 267,63±56,36 ng/mL in the infertile group. Among infertile patient groups, no significant difference was observed regarding the relationship between seminal leptin levels and age, volume, sperm count and motility.Conclusion: In our study it was demonstrated that, serum leptin levels decrease in oligoasthenoteratozoospermic infertile males, while there is not a significant relation with the motility of sperm. In order to use seminal leptin levels as a diagnostic tool more extensive studies are needed.
Anahtar Kelime:

Konular:
Fen > Tıp > Kadın Hastalıkları ve Doğum
Fen > Tıp > Pediatri
Fen > Tıp > Cerrahi
Fen > Tıp > Genel ve Dahili Tıp
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • 1. Jianhua Guo, Yang Zhao, Weiying Huang, Wei Hu, Jian-- jun Gu, Chuhong Chen, Juan Zhou, Yubing Peng, Min Gong, Zhong Wang., Sperm motility inversely correlates with seminal leptin levels in idiopathic asthenozoospermia. Int Clin Exp Med 2014; 7(10):3550--3555.
  • 2. Sigman M, Howards SS: Male infertility: Campbell Uro-- logy. (Walsh PC, Retik AB, Vaughan ED, Wein AJ, ed). Seventh edition. Philadelphia, Saunders company. 998:1287--1330.
  • 3. Sokol RZ: Endocrinology and male infertility Infertility and Reproductive Medicine Clinics of North America. (Werthman PE, ed). Philadelphia, Saunders. 1999:427--434
  • 4. Homan GF, Davies and Norman R. The impact oflifestyle factors on reproductive performance in the general populati-- on and those undergoing infertility treatment: review. Hum Reprod Update 2007; 3: 209--223.
  • 5. Fernandez CD, Bellentani FF, Fernandes GS, Perobelli JE, Favareto AP, Nascimento AF, Cicogna AC and Kempinas WD. Diet--induced obesity in rats leads to decrease in sperm moti-- lity. Reprod Biol Endocrinol 2011; 9: 32.
  • 6. Rosenbaum M, Leibel RL: The role of leptin in human physi-- ology. NEnglJMed,I999; 341: 913--915.
  • 7. Jacobs HS, Seibell MM: Polycystic Ovary Syndrome. Inferti-- lity. (Seibell MM, ed). Second edition. Appleton, I997; 121--133.
  • 8. Ando and Aquila S. Arguments raised by the recent disco-- very that insulin and leptin are expressed in and secreted by human ejaculated spermatozoa. Mol Cell Endocrinol 2005; 245 1--6.
  • 9. Farooqi IS, Jebb SA, Langmack G, etal: Eflects ofre--combi-- nant leptin therapy in child with congenital leptin deficiency. NEngl JMed, 1999; 341: 879--884.
  • 10. Nazian SJ, Cameron DF: Temporal relation between leptin and various indices ofsexual maturation in the male rat. And-- rol, I999; 20: 487--491.
  • 11. Wang J, Liu R, Hawkins M, Barzilai N, Rossetti L:Anut-- rient--sensing pathway regulates leptin gene expression in muscle andfat. Nature 998; 18: 684--688.
  • 12. Bado A, Levasseur S, Attoub S, Kermorgant S, Laigneau JP, Bortoluzzi MN, et al; The stomach is source of leptin. Nature 998; 20: 790-- 793.
  • 13. Hoggard N, Hunter L, Duncan JS, Williams LM, Trayhurn P, Mercer JG. Leptin and leptin receptor mRNA and protein expression in the murinefetus andplacenta. Proc Natl Acad Sci 1997; 94: 11073-- 8.
  • 14. Karlsson C, Lindell K, Svensson E, Bergh C, Lind P, Billig H, Carlsson LMS, Carlsson .Expression offunctional lep-- tin receptors in the human ovary. Clin Endocrinol Metab 1997;82: 4144--8.
  • 15. Chehab FF Mounzih K, Lu R, Lim ME. Early onset of reproductivefunction in normalfemale mice treated with leptin. Science 1997;2 75: 88--90.
  • 16. Barash IA, Cheung CC, Weigle DS, et al. Leptin is me-- tabolic signal to the reproductive system. Endocrinology, 1996;137: 3144--3147.
  • 17. Baskin DG, Hahn TM, Schwartz MW Leptin sensitive neurons in the hypothalamus. Horm Metab Res 199931: 345-- 350.
  • 18. Haron MN, D'Souza UJ, Jaafar H, Zakaria R, Jeet Singh H. Exogenous leptin administration decreases sperm count and increases the fraction of abnormal sperm in adult rats. Fertil Steril 2009; 93: 322-324.
  • 19. Aquila S, Gentile M, Middea M, Catalano S, Morelli C, Pez-- zi V, et al. Leptin secretion by human ejaculated spermatozoa. Clin Endocrinol Metab 2005; 90: 753--4 761
  • 20. Li HW, Chiu PC, Cheung MP, Yeung WS, WS. Eflect of leptin on motility, capacitation and acrosome reaction of hu-- man spermatozoa. 1nt Androl 2008; 32: 687-- 694.
  • 21. Glander HJ, Lammert A, Paasch U, Glasow A, Kratzsch J. Lepin exits in tubuli seminiferi and in seminal plasma. Andro-- logia 2002; 34:227--233.
  • 22. Moschos S, Chan JL, Mantzoros CS. Leptin and reproducti-- on: review. Fertil Steril 2002; 3: 433--444.
  • 23. Caprioa M, Fabbrinic E, Riccid G, Bascianic S, Gnessic L, Arizzic M, et al. Ontogenesis of leptin receptor in rat leydig cells. Biol Reprod 2003; 68: 1199--1207.
  • 24. Lange Consiglio A, Dell 'Aquila ME, Fiandanese N, Amb-- ruosi B, Cho YS, Bosi G, et al. Eflects of leptin on in vitro ma-- CİLT: 48 YIL: 2017 SAY1:2 turation, fertilization and embryonic cleavage after 1CS1 and early developmental expression ofleptin (Ob) and leptin recep-- tor (ObR) proteins in the horse. Reprod Biol Endocrinol 2009; 7:113.
  • 25. Smith GD, Jackson LM, Foster DL. Leptin regulation of reproductive function and fertility. heriogenology 2002; 57: 73--86.
  • 26. Steinman N, Gamzu R, Yogev L, et al: Serum leptin concent-- rations are higher in azoospermic than in normozoospermic men. Fertil Steril 2001; 75: 821--822, 2001.
  • 27. Tunç L, TokgözH, Kordan Y, Biri H, Karaoğlan Ü, Bozkırlı İ. Oligospermik erkek infertilitesinde Leptin 'in yeri. urk Uro-- loji Dergisi 2005;31(2):207--211.
  • 28. Jorsaraei A, Shibahara H, Ayustawati, Hirano Y, Suzuki Marzony ET Zainalzadeh M, Suzuki The Leptin concent-- rations in seminal plasma of men and its relationship to semen parameters. Iranian Journal of Reproductive Medicine 2010; 8:3. 95--100.
  • 29. Caprio M, Fabbrini E, Isiodori AM, Aversa A, Fabbri A. Leptin in reproduction. Trends Endocrinol Metab 2001; 12: 65--72.
  • 30. Camina JP, Lage M, Menendez C, Grana M, Garcia--Deve-- sa J, Dieguez C, et al. Evidence offree leptin in human seminal plasma. Endocrine 2002; 7: 169--1 74.
APA AYLA Ş, KESKİN İ, YELKE VARLI T, ÖNEL T, KARAHÜSEYİNOĞLU S (2017). Erkek İnfertilitesinde Seminal Leptin Seviyelerinin Semen Parametreleri ve Dna Fragmantasyonu İle İlişkisi. Zeynep Kamil Tıp Bülteni, 48(2), 39 - 43.
Chicago AYLA ŞULE,KESKİN İLKNUR,YELKE VARLI Tuba,ÖNEL Tuğçe,KARAHÜSEYİNOĞLU SERÇİN Erkek İnfertilitesinde Seminal Leptin Seviyelerinin Semen Parametreleri ve Dna Fragmantasyonu İle İlişkisi. Zeynep Kamil Tıp Bülteni 48, no.2 (2017): 39 - 43.
MLA AYLA ŞULE,KESKİN İLKNUR,YELKE VARLI Tuba,ÖNEL Tuğçe,KARAHÜSEYİNOĞLU SERÇİN Erkek İnfertilitesinde Seminal Leptin Seviyelerinin Semen Parametreleri ve Dna Fragmantasyonu İle İlişkisi. Zeynep Kamil Tıp Bülteni, vol.48, no.2, 2017, ss.39 - 43.
AMA AYLA Ş,KESKİN İ,YELKE VARLI T,ÖNEL T,KARAHÜSEYİNOĞLU S Erkek İnfertilitesinde Seminal Leptin Seviyelerinin Semen Parametreleri ve Dna Fragmantasyonu İle İlişkisi. Zeynep Kamil Tıp Bülteni. 2017; 48(2): 39 - 43.
Vancouver AYLA Ş,KESKİN İ,YELKE VARLI T,ÖNEL T,KARAHÜSEYİNOĞLU S Erkek İnfertilitesinde Seminal Leptin Seviyelerinin Semen Parametreleri ve Dna Fragmantasyonu İle İlişkisi. Zeynep Kamil Tıp Bülteni. 2017; 48(2): 39 - 43.
IEEE AYLA Ş,KESKİN İ,YELKE VARLI T,ÖNEL T,KARAHÜSEYİNOĞLU S "Erkek İnfertilitesinde Seminal Leptin Seviyelerinin Semen Parametreleri ve Dna Fragmantasyonu İle İlişkisi." Zeynep Kamil Tıp Bülteni, 48, ss.39 - 43, 2017.