Deneysel Periferik Sinir Hasarında Kateşin Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi

DIVANLIOGL, Denizhan; AKDAG, Rifat; GUVENC, Yahya; KÖKSAL, İsmet; Alagöz, Fatih; Helvacıoğlu, Fatma; DALGIC, Ali; BELEN, Ahmed Deniz; CETINA, Nuri Eralp; YILDIRIM, Ali Erdem; TAKE KAPLANOĞLU, Gülnur

Deneysel Periferik Sinir Hasarında Kateşin Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi

Ali Erdem YILDIRIM, (Ankara Numune Research and Education Hospital, Department of Neurosurgery, Ankara, Turkey)

Ali DALGIÇ, (Ankara Numune Research and Education Hospital, Department of Neurosurgery, Ankara, Turkey)

Denizhan DIVANLIOGL, (Ankara Numune Research and Education Hospital, Department of Neurosurgery, Ankara, Turkey)

Rifat AKDAG, (Bursa Sevket Yilmaz Education and Research Hospital, Department of Neurosurgery, Bursa, Turkey)

Nuri Eralp CETINA, (3Cukurova University Hospital, Department of Neurosurgery, Adana, Turkey)

Fatih ALAGÖZ, (Ankara Numune Research and Education Hospital, Department of Neurosurgery, Ankara, Turkey)

Fatma HELVACIOĞLU, (Baskent University Hospital, Department of Histology and Embryology, Ankara, Turkey)

Gülnur TAKE KAPLANOĞLU, (Gazi University Hospital, Department of Histology and Embryology, Ankara, Turkey)

Yahya GUVENC, (Sincan State Hospital, Department of Neuorusurgery, Ankara, Turkey)

İsmet KÖKSAL, (Yenimahalle State Hospital, Department of Ortophedia, Ankara, Turkey)

Ahmed Deniz BELEN, (Ankara Numune Research and Education Hospital, Department of Neurosurgery, Ankara, Turkey)

Diğer Yazarlar

Turkish Neurosurgery

0 0

Yıl: 2015 Cilt: 25 Sayı: 3 Sayfa Aralığı: 453 - 460 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Deneysel Periferik Sinir Hasarında Kateşin Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi

Öz:

AMAÇ: Kateşinler polifenol grubundan olup başlıca kateşin, epikateşin, epigallokateşin, epigallokateşingallat (EGKG) gibi alt grup maddeler içermektedir. Çalışmada kateşinin temel metaboliti olan EGKG'ın, periferik sinir hasarı yapılmış sıçanlardaki etkisinin gösterilmesi amaçlanmıştır.yÖNTEM ve GErEÇLEr: Toplam 74 Albino-Wistar cinsi rat kontrol, travma, serum fizylojik (SF), 25 mg/kg, 50 mg/kg ve günlük tüketim ( travma öncesi 14 gün boyunca intraperitoneal 10 mg/kg EGKG verildi) EGKG grupları olmak üzere 6 gruba ayrıldı. İlk grup hariç diğer gruplarda siyatik sinire 50 gr/cm2 basıncı ile kapanan klip ile 1 dakika kompresyon hasarı yapıldı. Bütün travma gruplarında, 28 gün sonra sinir örnekleri alınarak biyokimyasal ve histopatolojik olarak incelendi. BuLGuLAr: Çalışmamızda günlük tüketim EGKG, 25mg/kg EGKG, 50mg/kg EGKG gruplarında istatistiksel olarak anlamlı düzeyde lipid peroksidasyonunun azaldığı ve özellikle travma+günlük tüketim EGKG, travma+25 mg/kg EGKG grularında histolojik olarak da dejenerasyon ve ödemin olumlu yönde azaldığı saptanmıştır. soNuÇ: Bu çalışma kateşin ve türevlerinin periferik sinir hasarı gibi nöronal hasarlanmalarda koruyucu etkisinin olduğu gösterilmiştir.

Anahtar Kelime:

Konular: Tıbbi Araştırmalar Deneysel Genel ve Dahili Tıp Biyokimya ve Moleküler Biyoloji

biochemical and histopathological Effects of Catechin on Experimental Peripheral Nerve Injuries

Öz:

AIM: Catechin is a type of polyphenol, along with epicatechin, epigallocatechin, and epigallocatechin-gallate (EGCG). This study aims to investigate the effect of EGCG, a major metabolite of catechin, which is the principle bioactive compound in green tea, on rats with peripheral nerve injury. MATErIAL and METHods: A total of 74 rats were divided into six groups, namely the control, the trauma, the normal saline, a 25mg/kg EGCG, a 50mg/kg EGCG and a daily consumption group (10mg/kg EGCG was given intraperitoneally for 14 days before the trauma). Except the first group, the other groups underwent a 1-minute sciatic nerve compression by clip with 50gr/cm2 pressure. Nerve samples were obtained at 28 day after trauma for the biochemical and histopathological analysis. rEsuLTs: Our study showed that the Daily consumption, 25mg/kg EGCG and 50mg/kg EGCG groups demonstrated statistically significant decreased lipid peroxidation levels and particularly daily consumption, and the 25mg/kg EGCG group showed a favourable reduction of degeneration and edema histologically.CoNCLusIoN: This study shows that Catechin and its derivatives have a protective effect on peripheral nerve injury.

Anahtar Kelime:

Konular: Tıbbi Araştırmalar Deneysel Genel ve Dahili Tıp Biyokimya ve Moleküler Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Baek WK, Jang BC, Lim JH, Kwon TK, Lee HY, Cho CH: Inhibitory modulation of ATP-sensitive potassium channels by gallateester moiety of (-)-epigallocatechin-3-gallate. Biochem Pharmacol 70: 1560-1567, 2005
2. Bastianetto S, Yao ZX, Papadopoulos V, Quirion R: Neuroprotective effects of green and black teas and their catechin gallate esters against beta-amyloid induced toxicity. Eur J Neurosci 23: 55-64, 2006
3. Brown JE, Khodr H, Hider RC, Rice-Evans CA: Structural dependence of flavonoid interactions with Cu2_ ions: Implications for their antioxidant properties. Biochem J 330: 1173-1178, 1998
4. Cadenas E, Packer L: Handbook of antioxidants. New York: Marcel Dekker İnc, 1996
5. Chaturvedi RK, Shukla S, Seth K, Chauhan S, Sinha C, Shukla Y, Agrawal AK: Neuroprotective and neurorescue effect of black tea extract in 6- hydroxydopamine-lesioned rat model of Parkinson's disease. Neurobiol Dis 22: 421-434, 2006
6. Cheeseman Kh, Slater TF: An introduction to free radical biochemistry. British Med Bulletin 149: 481-493, 1993
7. Dalluge JJ, Nelson BC: Determination of tea catechins. J Chromatogr A 881: 411-424, 2000
8. Doss MX, Potta SP, Hescheler J, Sachinidis A: Trapping of growth factors by catechins: A possible therapeutical target for prevention of proliferative diseases. J Nutr Biochem 16: 259-266, 2005
9. Fern R, Harrison PJ: The contribution of ischaemia and deformation to the conduction block generated by compression of the cat sciatic nerve. Exp Physiol 79: 583-592, 1994
10. Frei B, Higdon JV: Antioxidant activity of tea polyphenols in vivo: Evidence from animal studies. J Nutr 133: 3275- 3284, 2003
11. Fu SY, Gordon T: The cellular and molecular basis of peripheral nerve regeneration. Mol Neurobiol 14: 67-116, 1997
12. Gramza A, Korczak J: Tea constituents (Camellia sinensis L.) as antioxidants inlipid systems. Trends in Food Sci Technol 16: 351-358, 2005
13. Guo Q, Zhao B, Shen S, Hou J, Hu J, Xin W: ESR study on the structure-antioxidant activity relationship of tea catechins and their epimers. Biochim Biophys Acta 1427: 13-23, 1999
14. Gupta S, Saha B,Giri AK: Comparative antimutagenic and anticlatogenic effect of green tea and black tea: A review. Mutation research 512: 37-65, 2002
15. Gutteridge JMC: Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 41: 1819-1828, 1995
16. Haenen GR, Paquay JB, Korthouwer RE, Bast A: Peroxynitrite scavenging by flavonoids. Biochem Biophys Res Commun 236: 591-593, 1997
17. Hall ED, Braughler M: Effects of intravenous methylprednisolone on spinal cord lipid peroxidation and Na-K ATPase activity. J Neurosurg 57: 247- 253, 1982
18. Hong J, Smith TJ, Ho CT, August DA, Yang CS: Effects of purified gren and black tea polyphenols on cyclooxygenase- and lipoxygenase-dependent metabolism of arachidonic acid in human colon mucosa and colon tumor tissues. Biochem Pharmacol 62: 1175-1183, 2001
19. Igarashı T, Yabuki S, Kıkuchı S, Myers RR: Effect of acute nerve root compression on endoneurial fluid pressure and blood flow in rat dorsal root ganglia. J Orthop Res 23: 420-424, 2005
20. Kanje M, Skottner A, Lundborg G: Effects of growth hormone treatment on the regeneration of rat sciatic nerve. Brain Res 475: 254-258, 1998
21. Katiyar SK, Mukhtar H: Inhibition of phorbol ester tumor promoter 12- tetradecanoylphorbol- 13-acetate-caused inflammatory responses in SENCAR mouse skin by black tea polyphenols. Carcinogenesis 18: 1911-1916, 1997
22. Katunuma N, Ohashi A, Sano E, Ishimaru N, Hayashi Y, Murata E: Catechin derivatives: Specific inhibitor for caspases-3, 7 and 2, and the prevention of apoptosis at the cell and animal levels. FEBS Lett 580: 741-746, 2006
23. Levites Y, Youdim MB, Maor G, Mandel S: Attenuation of 6- hydroxydopamine (6-OHDA)-induced nuclear factor-kappaB (NF-kappaB) activation and cell death by tea extracts in neuronal cultures. Biochem Pharmacol 63: 21-29, 2002
24. Li Y, Bickel KD, Im MJ, Hu L, Dellon AL, Vander Kolk CA, Manson PN: Effects of deferoxamine on ischemia/reperfusion injury after peripheral nerve compression. Ann Plast Surg 36: 365- 369, 1996
25. Lundborg G, Dahlin LB: Anatomy, function, and pathophysiology of peripheral nerves and nerve compression. Hand Clin 12: 185-193, 1996
26. Macchi MM, Bruce JN: Human pineal physiology and functional significance of melatonin. Front Neuroendocrinol 25: 177-195, 2004
27. Mendoza-Wilson AM, Glossman-Mitnik D: Theoretical study of the molecular properties and chemical reactivity of (+)-catechin and (-)-epicatechin related to their antioxidant ability. J Mol Struct Theocem 761: 97-106, 2006
28. Nwuha V, Nakajima M, Tong J, Ichikawa S: Solubility study of green tea extracts in pure solvents and edible oils. J Food Eng 40: 161-165, 1999
29. Paquay JB, Haenen GR, Stender G, Wiseman SA, Tijburg LB, Bast A: Protection against nitric oxide toxicity by tea. J Agric Food Chem 48: 5768-5772, 2000
30. Persengiev S, Kanchev L, Vezenkova G: Circadian patterns of melatonin, corticosterone, and progesterone in male rats subjected to chronic stress: Effect of constant illumination. J Pineal Res 11: 57-62, 1991
31. Rabinovsky ED: The multifunctional role of IGF-1 in peripheral nerve regeneration. Neurol Res 26: 204-210, 2004
32. Rahman RM, Nair SM, Helps SC, Shaw OM, Sims NR, Rosengren RJ: Epigallocatechin gallate as an intervention for the acute treatment of cerebral ischemia. Neurosci Lett 382: 227-230, 2005
33. Ramon Y, Cajal S: Degeneration and regeneration of the nervous system. London: Oxford University Press, 1928
34. Schmelzer JD, Zochodne DW, Low PA: Ischemic and reperfusion injury of rat peripheral nerve. Proc Natl Acad Sci 86: 1639-1642, 1989
35. Seddon HJ: Three types of nerve injury. Brain 66: 237-288, 1943
36. Sutherland BA, Rahman RMA, Appleton I: Mechanisms of action of gren tea catechins, with a focus on ischemiainduced neurodegeneration. J Nutr Biochem 17: 291-306, 2006
37. Tang SZ, Kerry JP, Sheehan D, Buckley DJ, Morrissey PA: Antioxidative effect of dietary tea catechins on lipid oxidation of long term frozen chicken meat Science 57: 331-336, 2001
38. Van der Zee CE, Brakkee JH, Gispen WH: Putative neurotrophic factors and functional recovery from peripheral nerve damage in the rat. Br J Pharmacol 103: 1041-1046, 1991
39. Xuczaj W, Skrzydlewska E: Antioxidative properties of black tea. Prev Med 40: 910-918, 2005
40. Yilmaz Y: Novel uses of catechins in foods. Trends in Food Sci Technol 17: 64-71, 2006
41. Zaveri NT: Green tea and its polyphenolic catechins: Medicinal uses in cancer and noncancer applications. Life Sci 78: 2073- 2080, 2006
42. Zhang YL, Zhang PB, Qiu SD, Liu Y, Tian YF, Wang Y: Effects of ketamine-midazolam anesthesia on the expression of NMDA and AMPA receptor subunit in the peri-infarction of rat brain. Chin Med J 119: 1555-1562, 2006

APA	YILDIRIM A, DALGIC A, DIVANLIOGL D, AKDAG R, CETINA N, Alagöz F, Helvacıoğlu F, TAKE KAPLANOĞLU G, GUVENC Y, KÖKSAL İ, BELEN A (2015). Deneysel Periferik Sinir Hasarında Kateşin Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi. , 453 - 460.
Chicago	YILDIRIM Ali Erdem,DALGIC Ali,DIVANLIOGL Denizhan,AKDAG Rifat,CETINA Nuri Eralp,Alagöz Fatih,Helvacıoğlu Fatma,TAKE KAPLANOĞLU Gülnur,GUVENC Yahya,KÖKSAL İsmet,BELEN Ahmed Deniz Deneysel Periferik Sinir Hasarında Kateşin Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi. (2015): 453 - 460.
MLA	YILDIRIM Ali Erdem,DALGIC Ali,DIVANLIOGL Denizhan,AKDAG Rifat,CETINA Nuri Eralp,Alagöz Fatih,Helvacıoğlu Fatma,TAKE KAPLANOĞLU Gülnur,GUVENC Yahya,KÖKSAL İsmet,BELEN Ahmed Deniz Deneysel Periferik Sinir Hasarında Kateşin Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi. , 2015, ss.453 - 460.
AMA	YILDIRIM A,DALGIC A,DIVANLIOGL D,AKDAG R,CETINA N,Alagöz F,Helvacıoğlu F,TAKE KAPLANOĞLU G,GUVENC Y,KÖKSAL İ,BELEN A Deneysel Periferik Sinir Hasarında Kateşin Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi. . 2015; 453 - 460.
Vancouver	YILDIRIM A,DALGIC A,DIVANLIOGL D,AKDAG R,CETINA N,Alagöz F,Helvacıoğlu F,TAKE KAPLANOĞLU G,GUVENC Y,KÖKSAL İ,BELEN A Deneysel Periferik Sinir Hasarında Kateşin Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi. . 2015; 453 - 460.
IEEE	YILDIRIM A,DALGIC A,DIVANLIOGL D,AKDAG R,CETINA N,Alagöz F,Helvacıoğlu F,TAKE KAPLANOĞLU G,GUVENC Y,KÖKSAL İ,BELEN A "Deneysel Periferik Sinir Hasarında Kateşin Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi." , ss.453 - 460, 2015.
ISNAD	YILDIRIM, Ali Erdem vd. "Deneysel Periferik Sinir Hasarında Kateşin Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi". (2015), 453-460.

APA	YILDIRIM A, DALGIC A, DIVANLIOGL D, AKDAG R, CETINA N, Alagöz F, Helvacıoğlu F, TAKE KAPLANOĞLU G, GUVENC Y, KÖKSAL İ, BELEN A (2015). Deneysel Periferik Sinir Hasarında Kateşin Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi. Turkish Neurosurgery, 25(3), 453 - 460.
Chicago	YILDIRIM Ali Erdem,DALGIC Ali,DIVANLIOGL Denizhan,AKDAG Rifat,CETINA Nuri Eralp,Alagöz Fatih,Helvacıoğlu Fatma,TAKE KAPLANOĞLU Gülnur,GUVENC Yahya,KÖKSAL İsmet,BELEN Ahmed Deniz Deneysel Periferik Sinir Hasarında Kateşin Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi. Turkish Neurosurgery 25, no.3 (2015): 453 - 460.
MLA	YILDIRIM Ali Erdem,DALGIC Ali,DIVANLIOGL Denizhan,AKDAG Rifat,CETINA Nuri Eralp,Alagöz Fatih,Helvacıoğlu Fatma,TAKE KAPLANOĞLU Gülnur,GUVENC Yahya,KÖKSAL İsmet,BELEN Ahmed Deniz Deneysel Periferik Sinir Hasarında Kateşin Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi. Turkish Neurosurgery, vol.25, no.3, 2015, ss.453 - 460.
AMA	YILDIRIM A,DALGIC A,DIVANLIOGL D,AKDAG R,CETINA N,Alagöz F,Helvacıoğlu F,TAKE KAPLANOĞLU G,GUVENC Y,KÖKSAL İ,BELEN A Deneysel Periferik Sinir Hasarında Kateşin Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi. Turkish Neurosurgery. 2015; 25(3): 453 - 460.
Vancouver	YILDIRIM A,DALGIC A,DIVANLIOGL D,AKDAG R,CETINA N,Alagöz F,Helvacıoğlu F,TAKE KAPLANOĞLU G,GUVENC Y,KÖKSAL İ,BELEN A Deneysel Periferik Sinir Hasarında Kateşin Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi. Turkish Neurosurgery. 2015; 25(3): 453 - 460.
IEEE	YILDIRIM A,DALGIC A,DIVANLIOGL D,AKDAG R,CETINA N,Alagöz F,Helvacıoğlu F,TAKE KAPLANOĞLU G,GUVENC Y,KÖKSAL İ,BELEN A "Deneysel Periferik Sinir Hasarında Kateşin Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi." Turkish Neurosurgery, 25, ss.453 - 460, 2015.
ISNAD	YILDIRIM, Ali Erdem vd. "Deneysel Periferik Sinir Hasarında Kateşin Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi". Turkish Neurosurgery 25/3 (2015), 453-460.