Cystic Echinococcosis (CE) is a highly significant parasitic zoonosis and can cause dramatic economic losses in humans and animals. In Turkey, the disease has been detected and highly prevalent in farm animals such as cattle, sheep and goat; however, there is very little research on CE in horses. The aim of this study is to detect the seroprevalance of CE in horses bred by the locals in Nevsehir province. For this purpose, blood samples were taken from 105 female horses of different races, aged from 3 to 24 and their sera were obtained. Partially purified cyst fluid antigen from sheep hydatid cysts was used as antigen in enzyme-linked immunosorbent assay (ELISA). At the end of the screening, anti-Echinococcus granulosus antibodies were detected in %4,54 (2/44) of horses aged 7 and under, and in %6,55 (4/61) of horses above 7 years of age. With the use of Chi-Square Test (X2), no statistically significant difference was found between age groups and seroprevalance (p ˃ 0,05). The overall seroprevalance rate was detected as %5,71 (6/105). The data obtained indicate that the horses in this region may be a source of infection for the final host, the canidae and are significant for the disease epidemiology. This study detects for the first time the seraprevalance of cystic echinoccocus in horses in Nevsehir province and contributes to the limited amount of literature related to this topic in Turkey