Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study

KARABACAK, Busra; KANDEMIR, Ersin; KARATAS, Idil; Şençelikel, Tuğçe; Helvacıoğlu, Fatma; PECEN, Ahmet; Dagdeviren, Attila; ERCAN, Ipek

Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study

Fatma HELVACIOĞLU, (Baskent University, Medical School, Department of Histology and Embryology, Ankara, Turkey)

Ersin KANDEMIR, (Baskent University, Medical School, Phase III Student, Ankara, Turkey)

Busra KARABACAK, (Baskent University, Medical School, Phase III Student, Ankara, Turkey)

Idil KARATAS, (Baskent University, Medical School, Phase III Student, Ankara, Turkey)

Ahmet PECEN, (Baskent University, Medical School, Phase III Student, Ankara, Turkey)

Ipek ERCAN, (Baskent University, Medical School, Phase III Student, Ankara, Turkey)

Tuğçe ŞENÇELİKEL, (Baskent University, Medical School, Department of Biostatistics, Ankara, Turkey)

Attila DAĞDEVİREN (Baskent University, Medical School, Department of Histology and Embryology, Ankara, Turkey)

Turkish Neurosurgery

52 2

Yıl: 2018 Cilt: 28 Sayı: 1 Sayfa Aralığı: 128 - 136 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study

Öz:

AIm: Creatine is an endogenous molecule synthesized in the liver, kidney and pancreas from glycine and arginine and is important for mitochondrial metabolism. It is widely used as a supplement for improving muscle mass and function for many years. As it is expected to prevent apoptosis and diminish oxidative stress, it is also studied in a number of neurodegenerative diseases for its beneficial effect in recent years. We studied the effect of creatine on the peripheral nerve injury in an experimental rat crush injury model to obtain ultrastructural evidence. mATERIAl and mEThODS: Animals were randomly divided into 3 groups having 5 animals in each group. Group 1 was the control group, Group 2 the trauma group and Group 3 the trauma+creatine group. The first group served as sham control. In group 2 and group 3, sciatic nerves of the rats received crush injury using aneurysm clips. In group 3, daily 2 g/kg creatine monohydrate was administered via gavage after the trauma. Nerve samples were obtained at the 28th day after trauma for light and electron microscopic evaluation. RESulTS: Our comparative analysis results suggest a possible positive effect of creatine supplement on peripheral nerve regeneration as statistical analysis revealed significant differences between group 2 and group 3. Though our finding does not represent a miracle of regenerative support, beneficial usage of creatine is documented in the present study. CONCluSION: Creatine supplement helps to diminish the harmful effects of peripheral nerve crush injury which is also supported by electron microscopy findings.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Helvacıoğlu F, KANDEMIR E, KARABACAK B, KARATAS I, PECEN A, ERCAN I, Şençelikel T, Dagdeviren A (2018). Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study. , 128 - 136.
Chicago	Helvacıoğlu Fatma,KANDEMIR Ersin,KARABACAK Busra,KARATAS Idil,PECEN Ahmet,ERCAN Ipek,Şençelikel Tuğçe,Dagdeviren Attila Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study. (2018): 128 - 136.
MLA	Helvacıoğlu Fatma,KANDEMIR Ersin,KARABACAK Busra,KARATAS Idil,PECEN Ahmet,ERCAN Ipek,Şençelikel Tuğçe,Dagdeviren Attila Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study. , 2018, ss.128 - 136.
AMA	Helvacıoğlu F,KANDEMIR E,KARABACAK B,KARATAS I,PECEN A,ERCAN I,Şençelikel T,Dagdeviren A Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study. . 2018; 128 - 136.
Vancouver	Helvacıoğlu F,KANDEMIR E,KARABACAK B,KARATAS I,PECEN A,ERCAN I,Şençelikel T,Dagdeviren A Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study. . 2018; 128 - 136.
IEEE	Helvacıoğlu F,KANDEMIR E,KARABACAK B,KARATAS I,PECEN A,ERCAN I,Şençelikel T,Dagdeviren A "Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study." , ss.128 - 136, 2018.
ISNAD	Helvacıoğlu, Fatma vd. "Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study". (2018), 128-136.

APA	Helvacıoğlu F, KANDEMIR E, KARABACAK B, KARATAS I, PECEN A, ERCAN I, Şençelikel T, Dagdeviren A (2018). Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study. Turkish Neurosurgery, 28(1), 128 - 136.
Chicago	Helvacıoğlu Fatma,KANDEMIR Ersin,KARABACAK Busra,KARATAS Idil,PECEN Ahmet,ERCAN Ipek,Şençelikel Tuğçe,Dagdeviren Attila Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study. Turkish Neurosurgery 28, no.1 (2018): 128 - 136.
MLA	Helvacıoğlu Fatma,KANDEMIR Ersin,KARABACAK Busra,KARATAS Idil,PECEN Ahmet,ERCAN Ipek,Şençelikel Tuğçe,Dagdeviren Attila Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study. Turkish Neurosurgery, vol.28, no.1, 2018, ss.128 - 136.
AMA	Helvacıoğlu F,KANDEMIR E,KARABACAK B,KARATAS I,PECEN A,ERCAN I,Şençelikel T,Dagdeviren A Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study. Turkish Neurosurgery. 2018; 28(1): 128 - 136.
Vancouver	Helvacıoğlu F,KANDEMIR E,KARABACAK B,KARATAS I,PECEN A,ERCAN I,Şençelikel T,Dagdeviren A Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study. Turkish Neurosurgery. 2018; 28(1): 128 - 136.
IEEE	Helvacıoğlu F,KANDEMIR E,KARABACAK B,KARATAS I,PECEN A,ERCAN I,Şençelikel T,Dagdeviren A "Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study." Turkish Neurosurgery, 28, ss.128 - 136, 2018.
ISNAD	Helvacıoğlu, Fatma vd. "Effect of Creatine on Rat Sciatic Nerve Injury: A Comparative Ultrastructural Study". Turkish Neurosurgery 28/1 (2018), 128-136.