Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro

Seker, Sukran

Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro

Şükran ŞEKER (Ankara University Stem Cell Institute and Ankara University Faculty of Science, Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara, TURKEY)

Hittite Journal of Science and Engineering

2 3

Yıl: 2018 Cilt: 5 Sayı: 1 Sayfa Aralığı: 1 - 5 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro

Öz:

Zinc oxide (ZnO) has been widely used in wound dressing, food additive and root canalfilling material owing to its antibacterial and antifungal properties. This study describespotential cytotoxic effect of ZnO on human periodontal ligament fibroblasts (hPDLFs) in vitro.ZnO was characterized using BET (Braun-Emmet-Teller) and DLS (dynamic light scattering)analyses. The hPDLFs were treated with 0.1, 1, 10, 50 and 100 μg/mL of ZnO for 6 h, 24 hand 48 h durations. After exposure to ZnO, phase contrast microscopy, transmission electronmicroscopy (TEM), mitochondrial function (MTT) and lactate dehydrogenase (LDH)assays were used to evaluate cell morphology, particle uptake, cell viability and membraneleakage. In addition, real time cell responses to ZnO exposure were monitored by impedancemeasurements. Results indicated that ZnO exposure caused toxic effect in a dose- and timedependentmanner when compared to control group, especially at 50 and 100 μg/mL.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Seker S (2018). Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro. , 1 - 5.
Chicago	Seker Sukran Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro. (2018): 1 - 5.
MLA	Seker Sukran Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro. , 2018, ss.1 - 5.
AMA	Seker S Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro. . 2018; 1 - 5.
Vancouver	Seker S Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro. . 2018; 1 - 5.
IEEE	Seker S "Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro." , ss.1 - 5, 2018.
ISNAD	Seker, Sukran. "Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro". (2018), 1-5.

APA	Seker S (2018). Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro. Hittite Journal of Science and Engineering, 5(1), 1 - 5.
Chicago	Seker Sukran Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro. Hittite Journal of Science and Engineering 5, no.1 (2018): 1 - 5.
MLA	Seker Sukran Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro. Hittite Journal of Science and Engineering, vol.5, no.1, 2018, ss.1 - 5.
AMA	Seker S Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro. Hittite Journal of Science and Engineering. 2018; 5(1): 1 - 5.
Vancouver	Seker S Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro. Hittite Journal of Science and Engineering. 2018; 5(1): 1 - 5.
IEEE	Seker S "Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro." Hittite Journal of Science and Engineering, 5, ss.1 - 5, 2018.
ISNAD	Seker, Sukran. "Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro". Hittite Journal of Science and Engineering 5/1 (2018), 1-5.