Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues

Kılıç, Murat; GÜRBÜZ, Nurdan; OĞUZTÜZÜN, Serpil; BULUŞ, Hakan; Bozer, Busra; Kaygin, Pinar; SIMSEK, Gulcin G; KOCDOGAN, Arzu K

Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues

Gulcin G SIMSEK, (University of Health Sciences, Kecioren Training and Research Hospital, Department of Pathology, Ankara)

Serpil OĞUZTÜZÜN, (Kirikkale University, Faculty of Arts and Sciences, Department of Biology, Kirikkale)

Busra BOZER, (Hitit University, Scientific Technical Research and Application Center, Corum)

MURAT KILIÇ, (Ankara University Vocational School of Health Services Department of Pharmacy, Ankara)

Arzu K KOCDOGAN, (Gelisim University, Health Services Vocational High School Pathology Laboratory Techniques Department, İstanbul)

Pınar KAYGIN, (Kirikkale University, Faculty of Arts and Sciences, Department of Biology, Kirikkale)

Nurdan GÜRBÜZ, (Kirikkale University, Faculty of Arts and Sciences, Department of Biology, Kirikkale)

Hakan BULUŞ (University of Health Sciences, Kecioren Training and Research Hospital, Department of General Surgery, Ankara, TURKEY)

Uluslararası Hematoloji-Onkoloji Dergisi

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Yıl: 2018 Cilt: 28 Sayı: 1 Sayfa Aralığı: 36 - 44 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues

Öz:

Bu çalışmada, 40 gastrik kanser hastasında, tümörlü ve tümör periferinde bulunan normal dokularında sitokrom P450 1A1 (CYP1A1), CYPB1, CYP2E1 ve glutatyon S-transferaz P1 (GSTP1), GSTT1, GSTO1, GSTK1 izozimlerinin immünohisıtokimyasal boyanma özellikleri araştırılmıştır. İmmünohistokimyasal çalışmalar için dokular, gastrik adenokarsinomalı 40 hastadan elde edildi. Hastaların tümör ve normal kontrol dokuları boyama yoğunluğuna göre karşılaştırıldı. Adenokarsinoma dokularında CYP ve GST izoenzimlerinin ekspresyonları arasındaki ilişki Mann Whitney-U testi ile ve klinikopatolojik veriler Spearman korelasyon rank testi ile incelendi. Gastrik kanserli dokularda CYP1B1, GSTT1, GSTO1 ve GSTK1 ekspresyonlarının normal dokulara oranla daha fazla olduğu istatistiksel olarak anlamlı bulundu (p< 0.05). Fakat, CYP1A1, CYP2E1 ve GSTP1 ekspresyonlarının normal dokulara oranla daha az olduğu istatistiksel olarak anlamlı bulundu (p< 0.05). Çalışılan CYP ve GST izozimlerinin ekspresyonları ile yaş ve cinsiyet arasında istatistiksel olarak anlamlı bir ilişki bulunamadı (p> 0.05). Gastrik adenokarsinomalı hastalarda, CYP1B1, GSTO1, GSTT1 ve GSTK1 protein ekspresyonları tümörlü dokuda, normal gastrik dokulardan daha yüksektir

Anahtar Kelime:

Konular: Onkoloji

İnsan Gastrik Tümörü ve Tümör Olmayan Dokulardaki CYP ve GST İzozimlerinin Ekspresyonları

Öz:

In this study we investigated the immunohistochemical staining characteristics of cytochrome P450 1A1 (CYP1A1), CYPB1, CYP2E1 and glutathione S-transferase P1 (GSTP1), GSTT1, GSTO1, GSTK1 isoenzymes in gastric tumor and surrounding tumor free (normal) gastric tissues from 40 patients. For immunohistochemical studies, tissues were obtained from 40 patients with gastric adenocarcinoma. Tumor and non-tumoral control tissues of patients were compared according to their staining intensity. Relationships between CYP and GST isoenzyme expressions in adenocarcinoma tissues were examined by the Mann Whitney-U test, and the clinicopathological data were examined by the Spearman’s Rank Correlation test. CYP1B1, GSTT1, GSTO1 and GSTK1 expressions in gastric cancer cells were significantly higher than those in gastric normal epithelial cells (p< 0.05). However, CYP1A1, CYP2E1 and GSTP1 expressions were not significantly higher in tumor epithelium than those in normal epithelium in human gastric adenocancer (p< 0.05). Among the studied CYPs and GSTs, there was not statistically significant association between the studied isoenzyme expressions and age, gender and tumor grade (p> 0.05). In patients with gastric adenocarcinoma, CYP1B1, GSTO1, GSTT1, and GSTK1 protein expressions are higher in tumor than normal gastric tissues

Anahtar Kelime:

Konular: Onkoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SIMSEK G, OĞUZTÜZÜN S, Bozer B, Kılıç M, KOCDOGAN A, Kaygin P, GÜRBÜZ N, BULUŞ H (2018). Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues. , 36 - 44.
Chicago	SIMSEK Gulcin G,OĞUZTÜZÜN Serpil,Bozer Busra,Kılıç Murat,KOCDOGAN Arzu K,Kaygin Pinar,GÜRBÜZ Nurdan,BULUŞ Hakan Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues. (2018): 36 - 44.
MLA	SIMSEK Gulcin G,OĞUZTÜZÜN Serpil,Bozer Busra,Kılıç Murat,KOCDOGAN Arzu K,Kaygin Pinar,GÜRBÜZ Nurdan,BULUŞ Hakan Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues. , 2018, ss.36 - 44.
AMA	SIMSEK G,OĞUZTÜZÜN S,Bozer B,Kılıç M,KOCDOGAN A,Kaygin P,GÜRBÜZ N,BULUŞ H Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues. . 2018; 36 - 44.
Vancouver	SIMSEK G,OĞUZTÜZÜN S,Bozer B,Kılıç M,KOCDOGAN A,Kaygin P,GÜRBÜZ N,BULUŞ H Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues. . 2018; 36 - 44.
IEEE	SIMSEK G,OĞUZTÜZÜN S,Bozer B,Kılıç M,KOCDOGAN A,Kaygin P,GÜRBÜZ N,BULUŞ H "Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues." , ss.36 - 44, 2018.
ISNAD	SIMSEK, Gulcin G vd. "Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues". (2018), 36-44.

APA	SIMSEK G, OĞUZTÜZÜN S, Bozer B, Kılıç M, KOCDOGAN A, Kaygin P, GÜRBÜZ N, BULUŞ H (2018). Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues. Uluslararası Hematoloji-Onkoloji Dergisi, 28(1), 36 - 44.
Chicago	SIMSEK Gulcin G,OĞUZTÜZÜN Serpil,Bozer Busra,Kılıç Murat,KOCDOGAN Arzu K,Kaygin Pinar,GÜRBÜZ Nurdan,BULUŞ Hakan Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues. Uluslararası Hematoloji-Onkoloji Dergisi 28, no.1 (2018): 36 - 44.
MLA	SIMSEK Gulcin G,OĞUZTÜZÜN Serpil,Bozer Busra,Kılıç Murat,KOCDOGAN Arzu K,Kaygin Pinar,GÜRBÜZ Nurdan,BULUŞ Hakan Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues. Uluslararası Hematoloji-Onkoloji Dergisi, vol.28, no.1, 2018, ss.36 - 44.
AMA	SIMSEK G,OĞUZTÜZÜN S,Bozer B,Kılıç M,KOCDOGAN A,Kaygin P,GÜRBÜZ N,BULUŞ H Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues. Uluslararası Hematoloji-Onkoloji Dergisi. 2018; 28(1): 36 - 44.
Vancouver	SIMSEK G,OĞUZTÜZÜN S,Bozer B,Kılıç M,KOCDOGAN A,Kaygin P,GÜRBÜZ N,BULUŞ H Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues. Uluslararası Hematoloji-Onkoloji Dergisi. 2018; 28(1): 36 - 44.
IEEE	SIMSEK G,OĞUZTÜZÜN S,Bozer B,Kılıç M,KOCDOGAN A,Kaygin P,GÜRBÜZ N,BULUŞ H "Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues." Uluslararası Hematoloji-Onkoloji Dergisi, 28, ss.36 - 44, 2018.
ISNAD	SIMSEK, Gulcin G vd. "Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues". Uluslararası Hematoloji-Onkoloji Dergisi 28/1 (2018), 36-44.