Does protocatechuic acid, a natural antioxidant, reduce renal ischemia reperfusion injury in rats?

AKŞİT, DİLEK; ASLAN, FİGEN; ÇIKMAN, ÖZTEKİN; YILDAR, Murat; BAŞBUĞ, Murat; Yüksel, Melih; AKSIT, HASAN; ÇAVDAR, Faruk

Does protocatechuic acid, a natural antioxidant, reduce renal ischemia reperfusion injury in rats?

Melih YÜKSEL, (Department of Emergency Medicine, Balıkesir University Faculty of Medicine, Balıkesir-Turkey)

Murat YILDAR, (Department of General Surgery, Balıkesir University Faculty of Medicine, Balıkesir-Turkey)

Murat BAŞBUĞ, (Department of General Surgery, Balıkesir University Faculty of Medicine, Balıkesir-Turkey)

Faruk ÇAVDAR, (Department of General Surgery, Balıkesir University Faculty of Medicine, Balıkesir-Turkey)

Öztekin ÇIKMAN, (Department of General Surgery, Çanakkale Onsekiz Mart University Faculty of Medicine, Çanakkale-Turkey)

Hasan AKŞİT, (Department of Biochemistry, Balıkesir University Faculty of Veterinary, Balıkesir-Turkey)

Figen ASLAN, (Department of Biochemistry, Balıkesir University Faculty of Veterinary, Balıkesir-Turkey)

Dilek AKŞİT (Department of Pharmacology and Toxicology, Balıkesir University Faculty of Veterinary, Balıkesir-Turkey)

Ulusal Travma ve Acil Cerrahi Dergisi

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Yıl: 2017 Cilt: 23 Sayı: 1 Sayfa Aralığı: 1 - 6 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Does protocatechuic acid, a natural antioxidant, reduce renal ischemia reperfusion injury in rats?

Öz:

AMAÇ: Protokateşuik asit (PCA) yeşil çay, fındık, badem başta olmak üzere birçok bitki, sebze ve meyvede yaygın olarak bulunan anti-oksidan özelliği olan basit bir fenol bileşiğidir. Bu çalışmada PCA'nın iskemi/reperfüzyon (IR) hasarı oluşturulan böbrekler üzerine olan etkilerini araştırmayı amaçladık. GEREÇ VE YÖNTEM: Sprague-Dawley cinsi sıçanlar rastgele dört gruba ayrıldı: (1) Sham, (2) Renal IR (3) Renal IR + Vehicle (4) Renal IR + PCA. Renal IR hasarı sağ nefrektomi yapıldıktan sonra 45 dakika boyunca sol renal pedikül klemplenip sonrasında üç saat boyunca reperfüzyon yapılarak indüklendi. Tedavi edici olarak kullanılan PCA renal iskemiden hemen önce 80 mg/kg dozunda intraperitoneal olarak uygulandı. Protokateşuik asiti çözmek için araç olarak %33'lük polietilen glikol kullanıldı. Sıçanlar sakrifiye edildikten sonra alınan kan örmeklerinde ve böbrek dokusunda total antioksidan kapasite (TAS), malondialdehit (MDA), süperoksitdismutaz (SOD), tümör nekroz faktör alfa (TNF-?), interlökin-6 (IL-6) seviyesi ölçüldü. Böbrek doku örneklerinde histopatolojik incelemeler yapılarak histopatolojik skorlama elde edildi. Apoptotik hücreleri görüntülemek için terminal deoxynucleotidyltransferase-mediated dUTP digoxigenin nick-end-labeling (TUNEL) boyama yapıldı. BULGULAR: Protokateşuik asitin serum MDA ve TNF-? ile böbrek MDA seviyelerini anlamlı olarak azalttığı, serum ve böbrek TAS ile SOD seviyelerini ise anlamlı olarak artırdığı görüldü. Histopatolojik skorlamanın PCA verilen grupta anlamlı olarak daha iyi idi. TARTIŞMA: Oksidatif stresi ve renal iskemi reperfüzyon hasarını azalttığını ortaya koyduğumuz PCA, IR hasarına karşı etkili bir ajan olarak kullanılabilir. Anahtar sözcükler: Antioksidanlar; iskemi reperfüzyon hasarı; malondialdehit; protokateşuik asit; serbest oksijen radikalleri.

Anahtar Kelime:

Konular: Tıbbi Araştırmalar Deneysel Üroloji ve Nefroloji Biyokimya ve Moleküler Biyoloji

Doğal bir antioksidan olan protokateşuik asit sıçanlarda renal iskemi reperfüzyon hasarını azaltıyor mu?

Öz:

BACKGROUND: Protocatechuic acid (PCA), which has antioxidant property, is a simple phenolic compound commonly found in many plants, vegetables, and fruits, notably in green tea and almonds. Present study was an investigation of the effects of PCA on rat kidney with ischemia/reperfusion (IR) injury.METHODS: Sprague-Dawley rats were randomly divided into 4 groups: (1) Sham, (2) Renal IR, (3) Renal IR+Vehicle, and (4) Renal IR+PCA. Renal reperfusion injury was induced by clamping renal pedicle for 45 minutes after right nephrectomy was performed, followed by reperfusion for 3 hours. Dose of 80 mg/kg PCA was intraperitoneally administered to 1 group immediately before renal ischemia; 33% polyethylene glycol was used as vehicle. Total antioxidant status (TAS), malondialdehyde (MDA), superoxide dismutase (SOD), tumor necrosis factor alpha (TNF-?), and interleukin-6 levels were measured in blood and kidney tissue samples taken from sacrificed rats. Kidney tissue samples were examined and scored histopathologically. Terminal deoxynucleotidyltransferase-mediated dUTP digoxigenin nick end labeling assay method was used to detect apoptotic cells.RESULTS: It was found that PCA significantly reduced serum MDA, TNF-?, and kidney MDA levels, while it increased serum and kidney TAS and SOD levels. Histopathological scores were significantly higher for the group given PCA.CONCLUSION: PCA reduced oxidative stress and can be used as an effective agent in treatment of renal IR injury.

Anahtar Kelime:

Konular: Tıbbi Araştırmalar Deneysel Üroloji ve Nefroloji Biyokimya ve Moleküler Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Yüksel M, YILDAR M, BAŞBUĞ M, ÇAVDAR F, ÇIKMAN Ö, AKSIT H, ASLAN F, AKŞİT D (2017). Does protocatechuic acid, a natural antioxidant, reduce renal ischemia reperfusion injury in rats?. , 1 - 6.
Chicago	Yüksel Melih,YILDAR Murat,BAŞBUĞ Murat,ÇAVDAR Faruk,ÇIKMAN ÖZTEKİN,AKSIT HASAN,ASLAN FİGEN,AKŞİT DİLEK Does protocatechuic acid, a natural antioxidant, reduce renal ischemia reperfusion injury in rats?. (2017): 1 - 6.
MLA	Yüksel Melih,YILDAR Murat,BAŞBUĞ Murat,ÇAVDAR Faruk,ÇIKMAN ÖZTEKİN,AKSIT HASAN,ASLAN FİGEN,AKŞİT DİLEK Does protocatechuic acid, a natural antioxidant, reduce renal ischemia reperfusion injury in rats?. , 2017, ss.1 - 6.
AMA	Yüksel M,YILDAR M,BAŞBUĞ M,ÇAVDAR F,ÇIKMAN Ö,AKSIT H,ASLAN F,AKŞİT D Does protocatechuic acid, a natural antioxidant, reduce renal ischemia reperfusion injury in rats?. . 2017; 1 - 6.
Vancouver	Yüksel M,YILDAR M,BAŞBUĞ M,ÇAVDAR F,ÇIKMAN Ö,AKSIT H,ASLAN F,AKŞİT D Does protocatechuic acid, a natural antioxidant, reduce renal ischemia reperfusion injury in rats?. . 2017; 1 - 6.
IEEE	Yüksel M,YILDAR M,BAŞBUĞ M,ÇAVDAR F,ÇIKMAN Ö,AKSIT H,ASLAN F,AKŞİT D "Does protocatechuic acid, a natural antioxidant, reduce renal ischemia reperfusion injury in rats?." , ss.1 - 6, 2017.
ISNAD	Yüksel, Melih vd. "Does protocatechuic acid, a natural antioxidant, reduce renal ischemia reperfusion injury in rats?". (2017), 1-6.

APA	Yüksel M, YILDAR M, BAŞBUĞ M, ÇAVDAR F, ÇIKMAN Ö, AKSIT H, ASLAN F, AKŞİT D (2017). Does protocatechuic acid, a natural antioxidant, reduce renal ischemia reperfusion injury in rats?. Ulusal Travma ve Acil Cerrahi Dergisi, 23(1), 1 - 6.
Chicago	Yüksel Melih,YILDAR Murat,BAŞBUĞ Murat,ÇAVDAR Faruk,ÇIKMAN ÖZTEKİN,AKSIT HASAN,ASLAN FİGEN,AKŞİT DİLEK Does protocatechuic acid, a natural antioxidant, reduce renal ischemia reperfusion injury in rats?. Ulusal Travma ve Acil Cerrahi Dergisi 23, no.1 (2017): 1 - 6.
MLA	Yüksel Melih,YILDAR Murat,BAŞBUĞ Murat,ÇAVDAR Faruk,ÇIKMAN ÖZTEKİN,AKSIT HASAN,ASLAN FİGEN,AKŞİT DİLEK Does protocatechuic acid, a natural antioxidant, reduce renal ischemia reperfusion injury in rats?. Ulusal Travma ve Acil Cerrahi Dergisi, vol.23, no.1, 2017, ss.1 - 6.
AMA	Yüksel M,YILDAR M,BAŞBUĞ M,ÇAVDAR F,ÇIKMAN Ö,AKSIT H,ASLAN F,AKŞİT D Does protocatechuic acid, a natural antioxidant, reduce renal ischemia reperfusion injury in rats?. Ulusal Travma ve Acil Cerrahi Dergisi. 2017; 23(1): 1 - 6.
Vancouver	Yüksel M,YILDAR M,BAŞBUĞ M,ÇAVDAR F,ÇIKMAN Ö,AKSIT H,ASLAN F,AKŞİT D Does protocatechuic acid, a natural antioxidant, reduce renal ischemia reperfusion injury in rats?. Ulusal Travma ve Acil Cerrahi Dergisi. 2017; 23(1): 1 - 6.
IEEE	Yüksel M,YILDAR M,BAŞBUĞ M,ÇAVDAR F,ÇIKMAN Ö,AKSIT H,ASLAN F,AKŞİT D "Does protocatechuic acid, a natural antioxidant, reduce renal ischemia reperfusion injury in rats?." Ulusal Travma ve Acil Cerrahi Dergisi, 23, ss.1 - 6, 2017.
ISNAD	Yüksel, Melih vd. "Does protocatechuic acid, a natural antioxidant, reduce renal ischemia reperfusion injury in rats?". Ulusal Travma ve Acil Cerrahi Dergisi 23/1 (2017), 1-6.