In Vitro Sitotoksisite Testleri

Tokur, Orhan; Aksoy, Abdurrahman

In Vitro Sitotoksisite Testleri

Orhan TOKUR, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Veterinerlik Farmakoloji ve Toksikolojisi Anabilim Dalı, Samsun, Türkiye.)

Abdurrahman AKSOY (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Veterinerlik Farmakoloji ve Toksikolojisi Anabilim Dalı, Samsun, Türkiye.)

Harran Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2017 Cilt: 6 Sayı: 1 Sayfa Aralığı: 112 - 118 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

In Vitro Sitotoksisite Testleri

Öz:

Kimyasal ve biyolojik maddeler ya da fiziksel etkenler, hücreleri değişik derecelerde etkileyerek sitotoksisiteye yol açabilirler. Bir maddenin biyolojik davranışının anlaşılabilmesi için hücreler üzerindeki toksik ya da non-toksik etkisinin belirlenmesi gereklidir. İn vitro sitotoksisite testleri, ilaç niteliği taşıyan veya toksik profili araştırılan maddelerin değerlendirilmesi amacıyla hücre kültüründe gerçekleştirilen ölçüm metodlarıdır. Bu testlerle çok sayıda maddenin kısa sürede analiz edilmesi mümkün olmakta ve yapılacak hayvan denemeleri için temel veriler elde edilebilmektedir. Farklı mekanizmalara ve hassasiyetlere sahip çok sayıda sitotoksisite testi bulunmakla birlikte bu derlemede, araştırmacılar tarafından sıkça tercih edilen tetrazolyum testleri (MTT, MTS, XTT, WST), LDH testi, alamalar mavisi testi ve biyolüminesans testleri ele alınmıştır

Anahtar Kelime:

Konular: Biyoloji Mikrobiyoloji Toksikoloji Biyokimya ve Moleküler Biyoloji Farmakoloji ve Eczacılık

In Vitro Cytotoxicity Assays

Öz:

Chemical, biological and physical factors may cause cytotoxicity by affecting cells at different degrees. To understand the biological characteristic of a compound, it is essential to determine its toxic or non-toxic effects. In vitro cytotoxicity assays (cell viability assays) are cell culture based measuring methods that usually used for either evaluating possible drug candidates or investigating cytotoxic profiles of some compounds. It is possible to assess many compounds in a short period of time and obtain fundamental information necessary for further animal experiments through this methods. There are many cell viability assay methods exist based on colorimetric, enzymatic, luminometric principals etc that have different mechanisms and varied sensitivity. The popular assays, such as MTT, MTS, XTT, WST, LDH enzyme assay, alamar blue assay, ATP bioluminescent assay have summarized in this rewiev

Anahtar Kelime:

Konular: Biyoloji Mikrobiyoloji Toksikoloji Biyokimya ve Moleküler Biyoloji Farmakoloji ve Eczacılık

Belge Türü: Makale Makale Türü: Derleme Erişim Türü: Erişime Açık

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APA	Tokur O, Aksoy A (2017). In Vitro Sitotoksisite Testleri. , 112 - 118.
Chicago	Tokur Orhan,Aksoy Abdurrahman In Vitro Sitotoksisite Testleri. (2017): 112 - 118.
MLA	Tokur Orhan,Aksoy Abdurrahman In Vitro Sitotoksisite Testleri. , 2017, ss.112 - 118.
AMA	Tokur O,Aksoy A In Vitro Sitotoksisite Testleri. . 2017; 112 - 118.
Vancouver	Tokur O,Aksoy A In Vitro Sitotoksisite Testleri. . 2017; 112 - 118.
IEEE	Tokur O,Aksoy A "In Vitro Sitotoksisite Testleri." , ss.112 - 118, 2017.
ISNAD	Tokur, Orhan - Aksoy, Abdurrahman. "In Vitro Sitotoksisite Testleri". (2017), 112-118.

APA	Tokur O, Aksoy A (2017). In Vitro Sitotoksisite Testleri. Harran Üniversitesi Veteriner Fakültesi Dergisi, 6(1), 112 - 118.
Chicago	Tokur Orhan,Aksoy Abdurrahman In Vitro Sitotoksisite Testleri. Harran Üniversitesi Veteriner Fakültesi Dergisi 6, no.1 (2017): 112 - 118.
MLA	Tokur Orhan,Aksoy Abdurrahman In Vitro Sitotoksisite Testleri. Harran Üniversitesi Veteriner Fakültesi Dergisi, vol.6, no.1, 2017, ss.112 - 118.
AMA	Tokur O,Aksoy A In Vitro Sitotoksisite Testleri. Harran Üniversitesi Veteriner Fakültesi Dergisi. 2017; 6(1): 112 - 118.
Vancouver	Tokur O,Aksoy A In Vitro Sitotoksisite Testleri. Harran Üniversitesi Veteriner Fakültesi Dergisi. 2017; 6(1): 112 - 118.
IEEE	Tokur O,Aksoy A "In Vitro Sitotoksisite Testleri." Harran Üniversitesi Veteriner Fakültesi Dergisi, 6, ss.112 - 118, 2017.
ISNAD	Tokur, Orhan - Aksoy, Abdurrahman. "In Vitro Sitotoksisite Testleri". Harran Üniversitesi Veteriner Fakültesi Dergisi 6/1 (2017), 112-118.