Characterization of buchnericin LB produced by Lactobacillus buchneri LB

YILDIRIM, Metin; Yildirim, Zeliha

Characterization of buchnericin LB produced by Lactobacillus buchneri LB

Zeliha YILDIRIM, (Gaziosmanpaşa Üniversitesi, Ziraat Fakültesi, Gıda Mühendisliği Bölümü, Tokat, Türkiye)

Metin YILDIRIM (Girilmedi)

Turkish Journal of Biology

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Yıl: 2001 Cilt: 25 Sayı: 1 Sayfa Aralığı: 73 - 82 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Characterization of buchnericin LB produced by Lactobacillus buchneri LB

Öz:

Lactobacillus buchneri LB isolated from a commercially fermented vegetable product produced a bacteriocin-designated buchnericin LB. It was found that buchnericin LB was heat-stable (90-121oC for 15 min) and hydrolyzed by proteases, $alpha$-chymotrypsin, trypsin, papain, proteinase K and pepsin. However, it was observed that it was resistant to catalase, peroxidase, lipase, amylases and organic solvents. Also, it was found that it retained its biological activity after exposure to pH 2.0-9.0 and after storage at -20o and -70oC. It was determined that buchnericin LB had a wide inhibitory spectrum, being inhibitory to the Listeria, Bacillus, Enterococcus, Micrococcus, Lactobacillus, Leuconostoc, Pediococcus, and Streptococcus species tested, and its molecular weight was determined to be about 3.5-4.5 kDa by tricine-SDS-PAGE.

Anahtar Kelime: molecular weight inhibition characterization antimicrobial properties bacteriocins heat enzymes Lactobacillus buchneri hydrolysis

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Yildirim Z, YILDIRIM M (2001). Characterization of buchnericin LB produced by Lactobacillus buchneri LB. , 73 - 82.
Chicago	Yildirim Zeliha,YILDIRIM Metin Characterization of buchnericin LB produced by Lactobacillus buchneri LB. (2001): 73 - 82.
MLA	Yildirim Zeliha,YILDIRIM Metin Characterization of buchnericin LB produced by Lactobacillus buchneri LB. , 2001, ss.73 - 82.
AMA	Yildirim Z,YILDIRIM M Characterization of buchnericin LB produced by Lactobacillus buchneri LB. . 2001; 73 - 82.
Vancouver	Yildirim Z,YILDIRIM M Characterization of buchnericin LB produced by Lactobacillus buchneri LB. . 2001; 73 - 82.
IEEE	Yildirim Z,YILDIRIM M "Characterization of buchnericin LB produced by Lactobacillus buchneri LB." , ss.73 - 82, 2001.
ISNAD	Yildirim, Zeliha - YILDIRIM, Metin. "Characterization of buchnericin LB produced by Lactobacillus buchneri LB". (2001), 73-82.

APA	Yildirim Z, YILDIRIM M (2001). Characterization of buchnericin LB produced by Lactobacillus buchneri LB. Turkish Journal of Biology, 25(1), 73 - 82.
Chicago	Yildirim Zeliha,YILDIRIM Metin Characterization of buchnericin LB produced by Lactobacillus buchneri LB. Turkish Journal of Biology 25, no.1 (2001): 73 - 82.
MLA	Yildirim Zeliha,YILDIRIM Metin Characterization of buchnericin LB produced by Lactobacillus buchneri LB. Turkish Journal of Biology, vol.25, no.1, 2001, ss.73 - 82.
AMA	Yildirim Z,YILDIRIM M Characterization of buchnericin LB produced by Lactobacillus buchneri LB. Turkish Journal of Biology. 2001; 25(1): 73 - 82.
Vancouver	Yildirim Z,YILDIRIM M Characterization of buchnericin LB produced by Lactobacillus buchneri LB. Turkish Journal of Biology. 2001; 25(1): 73 - 82.
IEEE	Yildirim Z,YILDIRIM M "Characterization of buchnericin LB produced by Lactobacillus buchneri LB." Turkish Journal of Biology, 25, ss.73 - 82, 2001.
ISNAD	Yildirim, Zeliha - YILDIRIM, Metin. "Characterization of buchnericin LB produced by Lactobacillus buchneri LB". Turkish Journal of Biology 25/1 (2001), 73-82.