The cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa L.

RENCÜZOĞLU, Eyyüp; ÇAKMAK, Tonguz; İLA, Hasan Basri; TOPAKTAŞ, Mehmet; KAYRALDIZ, Ahmet

The cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa L.

Eyyüp RENCÜZOĞLU, (Çukurova Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Adana, Türkiye)

Ahmet KAYRALDIZ, (Girilmedi)

Hasan Basri İLA, (Girilmedi)

Tonguz ÇAKMAK, (Girilmedi)

Mehmet TOPAKTAŞ (Girilmedi)

Turkish Journal of Biology

3 0

Yıl: 2001 Cilt: 25 Sayı: 4 Sayfa Aralığı: 361 - 370 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

The cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa L.

Öz:

The effects of sodium metabisulfite (SMB) on mitosis were investigated in Allium cepa L. The roots of A. cepa were treated with SMB concentrations of 7.5 mg/lt, 15 mg/lt and 30 mg/lt for 10- and 20-hour treatment periods. SMB significantly decreased the mitotic index (MI) at all concentrations and treatment periods. While the decreasing of the MI was dose-dependent at 10 hours treatment time, SMB increased the mitotic abnormalities dose dependently.

Anahtar Kelime: root tips sodium metabisulfite genetics Allium cepa antioxidants food preservatives Allium chromosomes cells mitosis chromosome analysis cytogenetics mitotic index

Konular: Biyoloji

Gıdalarda antimikrobiyal madde olarak kullanılan sodyum metabisulfit'in Allium cepa L.7nin kök ucu hücrelerinde sitogenetik etkileri

Öz:

Anahtar Kelime: Allium kromozomlar hücre mitoz kromozom analizleri sitogenetik mitotik indeks kök uçları sodyum metabisülfit genetik Allium cepa antioksidanlar gıda koruyucuları

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Diğer Erişim Türü: Erişime Açık

1.Nagao M., Honda M., Seino Y., Yahagi T., Kawachi T., Mutagenicities of protein pyrolates. Cancer Let., 2, 335-339, 1977.
2.Ishidate M. Jr., Odashima S., Chromosome test with 134 compounds on Chinese hamster cells in vitro-a screen-ing for chemical carcinogens. Mutation Res., 48, 337-353, 1977.
3.Matsuoka A., Hayashi M., Ishidate M. Jr., Chromosomal aberration test on 29 chemicals combined with S9 mixin vitro. Mutation Res., 66, 277-290, 1979.
4.Luca D., Luca V., Cotor F., Raileanu L., In vivo and in vitro cytogenetic damage induced by sodium nitrite. MutationRes., 189, 333-339, 1987.
5.Mukherjee A., Giri A. K., Talukder G., Sharma A., Sister chromatid exchanges and micronuclei formations inducedby sorbic acid and sorbic acid-nitrite in vivo in mice. Toxicol. Let., 42, 47-53, 1988.
6.Njagi G. D., Gopalan H. N., Cytogenetic effects of the food preservatives-sodium benzoate and sodium sulphite onVicia faba root meristems. Mutation Res., 102, 213-219, 1982.
7.Hasegawa M. M., Nishi Y., Ohkawa Y., Inui N., Effects of sorbic acid and its salts on chromosome aberrations, sis-ter chromatid exchanges and gene mutations in cultured Chinese hamster cells. Food. Chem. Toxicol., 22, 501-507, 1984.
8.Abe S., Sasaki M., Chromosome aberrations and sister chromatid exchanges in Chinese hamster cells exposed tovarious chemicals. J. Natl. Cancer Inst., 58, 1635-1643, 1977.
9.Ishidate M. Jr., Sofuni T., Yoshikawa K., Hayashi M., Nohmi T., Sawada M., Matsuoka A., Primary mutagenicityscreening of food additives currently used in Japan. Food. Chem. Toxicol., 22, 623-636, 1984.
10. Akın A., Sümer S., The mutagenic effects of sodium nitrite and monosodium glutamate used as food additivesdemonstrated by the Salmonella microsome test system. Microbiol. Bul., 25, 94-107, 1991.
11. Munzer R., Guigas C., Renner H. W., Re-examination of potassium sorbate and sodium sorbate for possible geno-toxic potential. Food. Chem. Toxicol., 28, 397-401, 1990.
12. Schlatter J., Wurgler F. E., Kranzlin R., Maier P., Holliger E., Grafu U., The potential genotoxicity of sorbates:effects on cell cycle in vitro in V79 cells and somatic mutations in Drosophila. Food. Chem. Toxicol., 30, 843-851, 1992.
13. The Ministry of Agriculture of Turkey, Food Codex Instructions, Dünya Publications, 197pp, 1997.
14. Meng Z., Zhang B., Polymerase chain reaction-based deletion screening of bisulfite enhanced gpt-mutants in CHO-AS52 cells. Mutation Res., 425, 81-85, 1999.
15. Pagano, D.A., Zeiger E., Conditions affecting the mutagenicity of sodium bisulfite in Salmonella typhimurium.Mutation Res., 179, 159-166, 1987.
16. Meng, Z., Zhang, L., Cytogenetic damage induced in human lymphocytes by sodium bisulfite. Mutation Res., 298,63-69, 1992.
17. Darlington C. D., La Cour L. F., Methoden der chromosomen-untersuchung, franck’sche verlagshandlung, W.Keller und Co., Stuttgart, 1963.
18. Epel, D., The effects of carbon monoxide inhibition of ATP level and the date of mitosis in Sea Urchin egg. J. Cell.Biol., 17, 315-319, 1963.
19. Jain, A., K., Andsorbhoy, R. K., Cytogenetical studies on the effects of some chlorinated pesticides III. Concludingremarks. Cytologia, 53, 427-436, 1988.
20. Jung R., Cojocel C., Muller W., Bottger D., Luck E., Evaluation of the genotoxic potential of sorbic acid and potas-sium sorbate. Food. Chem.Toxicol., 30, 1-7, 1992.
21. Walker R., Toxicology of sorbic acid and sorbates. Food. Addit. Contam., 7, 671-676, 1990.
22. Tsuchiya T., Yamaha T., Depletion of the reduced glutathione level in the liver and production of the mutagens inthe intestine in the mice inducing hepatome by feeding on a high level dose of sorbic acid. Mutation Res., 130,267-272, 1984.
23. Kinashi Y., Masunaga S., Takagaki M., Ono K., Mutagenic effects at HPGRT locus induced in Chinese hamsterovary cells by thermal neutrons with or without boron compound. Mutation Res., 377, 211-215, 1997.
24. McGregor D. B., Brown A., Cattanach P., Edwards I., McBridge D., Riach C., Caspary W. J., Response of theL5178Y tk+/tk- mouse lymphoma cell forward mutation assay: III. 72 coded chemicals. Environ. Mol.Mutagen.,12, 85-154, 1988.
25. Bhanot O.S., Chambers R. W., Bisulfite induced C changed to U transitions in yeast alanine tRNA. J. Biol. Chem.,252, 2551-2559, 1977.
26. Bhanot O.S., Aoyagi S., Chambers R. W., Bisulfite induced C changed to U transitions in yeast valine tRNA. J. Biol.Chem., 252, 2566-2574, 1977.
27. Simmons R.R., Friedberg E.C., Enzymatic degradation of uracil containing deoxyribonucleic acid. V. Survival ofEscherichia coli and coliphages treated with sodium bisulfite. J. Bacteriol., 137, 1243-1252, 1979.
28. Chen H., Shaw B. R., Bisulfite induces tandem double CC-->TT mutations in double strand DNA. 2. kinetics ofcytosine deamination. Biochemistry, 33, 4121-4129, 1994.
29. Molander J., Hurskainen P., Hovinen J., Lahti M., Lyonnberg H., Bisulfite ion catalysed transamination of cytosineresidues with alpha, omega alkanediamines: the effect of chain length on the reaction kinetics. Bioconjug Chem.,4, 362-365, 1993.
30. Hayatsu H., Shiragami M., Reaction of bisulfite with the 5-hydoxymethyl group in pyrimidines and in phage DNAs.Biochemistry, 18, 532-637, 1979.
31. Knippers R., Schaffer K. P., Fanning E., Moleculare Genetik. George Thime Verlag, Stuttgart, New York, p. 422,1985.
32. Renner H. W., Wever J., Attempts to induce cytogenetic effects with sulphite in sulphite oxidase-deficient Chinesehamsters and mice. Food. Chem. Toxicol., 21,123-127, 1983.
33. Mavournin K. H., Blakey D. H., Cimino M. C., Salamone M. F., Heddle J. A., The in vivo micronucleus assay inmammalian bone marrow and peripheral blood. A report of the U. S. Environmental Protection Agency Gene-ToxProgram. Mutation Res., 239, 29-80, 1990

APA	RENCÜZOĞLU E, KAYRALDIZ A, İLA H, ÇAKMAK T, TOPAKTAŞ M (2001). The cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa L.. , 361 - 370.
Chicago	RENCÜZOĞLU Eyyüp,KAYRALDIZ Ahmet,İLA Hasan Basri,ÇAKMAK Tonguz,TOPAKTAŞ Mehmet The cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa L.. (2001): 361 - 370.
MLA	RENCÜZOĞLU Eyyüp,KAYRALDIZ Ahmet,İLA Hasan Basri,ÇAKMAK Tonguz,TOPAKTAŞ Mehmet The cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa L.. , 2001, ss.361 - 370.
AMA	RENCÜZOĞLU E,KAYRALDIZ A,İLA H,ÇAKMAK T,TOPAKTAŞ M The cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa L.. . 2001; 361 - 370.
Vancouver	RENCÜZOĞLU E,KAYRALDIZ A,İLA H,ÇAKMAK T,TOPAKTAŞ M The cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa L.. . 2001; 361 - 370.
IEEE	RENCÜZOĞLU E,KAYRALDIZ A,İLA H,ÇAKMAK T,TOPAKTAŞ M "The cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa L.." , ss.361 - 370, 2001.
ISNAD	RENCÜZOĞLU, Eyyüp vd. "The cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa L.". (2001), 361-370.

APA	RENCÜZOĞLU E, KAYRALDIZ A, İLA H, ÇAKMAK T, TOPAKTAŞ M (2001). The cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa L.. Turkish Journal of Biology, 25(4), 361 - 370.
Chicago	RENCÜZOĞLU Eyyüp,KAYRALDIZ Ahmet,İLA Hasan Basri,ÇAKMAK Tonguz,TOPAKTAŞ Mehmet The cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa L.. Turkish Journal of Biology 25, no.4 (2001): 361 - 370.
MLA	RENCÜZOĞLU Eyyüp,KAYRALDIZ Ahmet,İLA Hasan Basri,ÇAKMAK Tonguz,TOPAKTAŞ Mehmet The cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa L.. Turkish Journal of Biology, vol.25, no.4, 2001, ss.361 - 370.
AMA	RENCÜZOĞLU E,KAYRALDIZ A,İLA H,ÇAKMAK T,TOPAKTAŞ M The cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa L.. Turkish Journal of Biology. 2001; 25(4): 361 - 370.
Vancouver	RENCÜZOĞLU E,KAYRALDIZ A,İLA H,ÇAKMAK T,TOPAKTAŞ M The cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa L.. Turkish Journal of Biology. 2001; 25(4): 361 - 370.
IEEE	RENCÜZOĞLU E,KAYRALDIZ A,İLA H,ÇAKMAK T,TOPAKTAŞ M "The cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa L.." Turkish Journal of Biology, 25, ss.361 - 370, 2001.
ISNAD	RENCÜZOĞLU, Eyyüp vd. "The cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa L.". Turkish Journal of Biology 25/4 (2001), 361-370.