Yıl: 2017 Cilt: 7 Sayı: 2 Sayfa Aralığı: 97 - 105 Metin Dili: Türkçe

Hypericum retusum Aucher’in Hücre Süspansiyon Kültürlerinin Optimizasyonu ve Fenolik Bileşen İçeriğinin İncelenmesi

Öz:
Hypericum retusum tıbbi bitki olarak potansiyel bir öneme sahiptir. Bu çalışmada, 6-benziladenin (BAP) ve kinetin (KİN)'in 2,4-diklorofenoksiasetik asit (2,4-D) ile kombinasyonlarının, farklı sukroz konsantrasyonlarının (15, 30 ve 50 g L-1), farklı ışık uygulamalarının (Sürekli karanlık, Sürekli aydınlık ve kontrol) ve farklı başlangıç pH değerlerinin (pH 4.5, 5.8 ve 6.5), H. retusum' un hücre süspansiyon kültürlerinde biyokütle birikimi ve farmakolojik olarak önemli beş adet fenolik bileşiğin (hiperisin, hiperosid, klorogenik asit, kuersetin ve psödohiperisin) miktarları üzerine etkisini araştırdık. Verilerimiz, H. retusum' un hücre süspansiyon kültürlerinde en iyi biyokütle birikiminin, 0.5 mg L-1 KİN+ 1.0 mg L-1 2,4-D ve 30 g L-1 ilaveli MS besi ortamında, pH'sı 5.8 ve sürekli karanlık şartlar altında olan ortamdan elde edildiğini ortaya koymuştur. En yüksek fenolik bileşik içeriği, 0.1 mg L-1 BAP+ 0.5 mg L-12,4-D ve 15 g L-1 sukroz ilaveli ve pH'sı 4.5 olan ortamdan elde edilmiştir. Farklı ışık uygulamalarına tabi tutulan kültürlerdeki fenolik bileşen içeriği ise uygulamalar arasında farklılık göstermiştir. Bu çalışmayla, H. retusum için, fenolik bileşiklerin geniş ölçekli üretimi için muhtemel bir teknoloji sunan yeni bir hücre süspansiyon kültür sistemi geliştirdik
Anahtar Kelime:

Medium Optimization and Investigation of Phenolic Contents in Cell Suspension Culture of Hypericum retusum Aucher

Öz:
Hypericum retusum has a potential value as a medicinal plant. In this study, we investigated the effects of different combination of 6-benzyladenine (BAP) and kinetin (KIN) with 2,4-dichlorophenoxyacetic acid (2,4-D), different sucrose concentrations (15, 30 and 50 g L-1) and different light conditions (continuous dark, continuous light and control) and initial pH values (pH 4.5, 5.8 ve 6.5) on the accumulation of biomass and the production of five pharmacologically important phenolic constituents (hypericin, hyperoside, chlorogenic acid, quercetin and pseudohypericin) in cell suspension culture of H. retusum. Our data suggested that the best biomass accumulations obtained from on MS medium supplemented with 0.5 mg L-1 KİN+1.0 mg L-1 2,4-D, 30 g L-1sucrose, 5.8 pH and under the darkness conditions in cell suspension culture of H. retusum. The highest phenolic compound contents were obtained from on MS medium containing 0.1 mg L-1 BAP+ 0.5 mg L-1 2,4-D at and 15 g L-1 sucrose. The phenolic compound contents of suspension culture under the different light conditions varied among the treatments. With this study, we have developed a novel cell suspension culture system for H. retusum, suggesting a possible technology for large-scale production of phenolics
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA SURMUŞ ASAN H, ÖZEN H, ONAY A (2017). Hypericum retusum Aucher’in Hücre Süspansiyon Kültürlerinin Optimizasyonu ve Fenolik Bileşen İçeriğinin İncelenmesi. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 7(2), 97 - 105.
Chicago SURMUŞ ASAN HİLAL,ÖZEN HASAN ÇETİN,ONAY AHMET Hypericum retusum Aucher’in Hücre Süspansiyon Kültürlerinin Optimizasyonu ve Fenolik Bileşen İçeriğinin İncelenmesi. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi 7, no.2 (2017): 97 - 105.
MLA SURMUŞ ASAN HİLAL,ÖZEN HASAN ÇETİN,ONAY AHMET Hypericum retusum Aucher’in Hücre Süspansiyon Kültürlerinin Optimizasyonu ve Fenolik Bileşen İçeriğinin İncelenmesi. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol.7, no.2, 2017, ss.97 - 105.
AMA SURMUŞ ASAN H,ÖZEN H,ONAY A Hypericum retusum Aucher’in Hücre Süspansiyon Kültürlerinin Optimizasyonu ve Fenolik Bileşen İçeriğinin İncelenmesi. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2017; 7(2): 97 - 105.
Vancouver SURMUŞ ASAN H,ÖZEN H,ONAY A Hypericum retusum Aucher’in Hücre Süspansiyon Kültürlerinin Optimizasyonu ve Fenolik Bileşen İçeriğinin İncelenmesi. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2017; 7(2): 97 - 105.
IEEE SURMUŞ ASAN H,ÖZEN H,ONAY A "Hypericum retusum Aucher’in Hücre Süspansiyon Kültürlerinin Optimizasyonu ve Fenolik Bileşen İçeriğinin İncelenmesi." Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 7, ss.97 - 105, 2017.
ISNAD SURMUŞ ASAN, HİLAL vd. "Hypericum retusum Aucher’in Hücre Süspansiyon Kültürlerinin Optimizasyonu ve Fenolik Bileşen İçeriğinin İncelenmesi". Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi 7/2 (2017), 97-105.