GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome

AKTAŞ, SAVAŞ; KARAGÜL, Meryem İlkay; OREKİCİ TEMEL, GÜLHAN; Yılmaz, Mustafa; COŞKUN YILMAZ, BANU

GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome

Meryem İlkay KARAGÜL, (Department of Histology and Embryology, Mersin University School of Medicine, Mersin, Turkey)

Savaş AKTAŞ, (Department of Histology and Embryology, Mersin University School of Medicine, Mersin, Turkey)

Banu Coşkun YILMAZ, (Department of Histology and Embryology, Mersin University School of Medicine, Mersin, Turkey)

Mustafa YILMAZ, (Department of Histology and Embryology, University of Health Sciences School of Medicine, İstanbul, Turkey)

GÜLHAN OREKİCİ TEMEL (Department of Biostatistics and Medical Informatics, Mersin University School of Medicine, Mersin, Turkey)

Balkan Medical Journal

5 2

Yıl: 2018 Cilt: 35 Sayı: 1 Sayfa Aralığı: 43 - 54 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome

Öz:

Background: Polycystic ovary syndrome is the mostfrequently seen endocrine disorder in women ofreproductive age with a prevalence of about 10%.Aims: To investigate the efficiency of growthdifferentiation factor 9 and bone morphogenetic protein15 during folliculogenesis in a dehydroepiandrosteroneinducedmouse Polycystic ovary syndrome model.Study Design: Animal experimentation.Methods: Mice were divided into 3 groups: control,vehicle and Polycystic ovary syndrome. Polycysticovary syndrome model mice were developed by theinjection of dehydroepiandrosterone dissolved in 0.1 mLof sesame oil. Ovarian tissues were examined for growthdifferentiation factor 9 and bone morphogenetic protein15 using immunofluorescent labelling and electronmicroscopic examinations.Results: The immunoreactivity of growth differentiationfactor 9 and bone morphogenetic protein 15 proteinsdecreased (p<0.05) in the Polycystic ovary syndromegroup (27.73±8.43 and 24.85±7.03, respectively)compared with the control group (33.72±11.22and 31.12±11.05, respectively) and vehicle group(33.95±10.75 and 29.99±10.72, respectively). Apoptoticchanges were observed in granulosa cells, lipid vacuolesincreased in Theca cells and thickening and irregularitieswere noted in the basal lamina of granulosa cells. Anincreased electron density in the zona pellucida in someof the multilaminar primary and secondary follicles inthe Polycystic ovary syndrome model was also observedat the ultrastructural level.Conclusion: These results suggest that the decrease in thegrowth differentiation factor 9 and bone morphogeneticprotein 15 expression initiated at the primary folliclestage effect the follicle development and zona pellucidastructure and may cause subfertility or infertility inPolycystic ovary syndrome

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KARAGÜL M, AKTAŞ S, COŞKUN YILMAZ B, Yılmaz M, OREKİCİ TEMEL G (2018). GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome. , 43 - 54.
Chicago	KARAGÜL Meryem İlkay,AKTAŞ SAVAŞ,COŞKUN YILMAZ BANU,Yılmaz Mustafa,OREKİCİ TEMEL GÜLHAN GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome. (2018): 43 - 54.
MLA	KARAGÜL Meryem İlkay,AKTAŞ SAVAŞ,COŞKUN YILMAZ BANU,Yılmaz Mustafa,OREKİCİ TEMEL GÜLHAN GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome. , 2018, ss.43 - 54.
AMA	KARAGÜL M,AKTAŞ S,COŞKUN YILMAZ B,Yılmaz M,OREKİCİ TEMEL G GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome. . 2018; 43 - 54.
Vancouver	KARAGÜL M,AKTAŞ S,COŞKUN YILMAZ B,Yılmaz M,OREKİCİ TEMEL G GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome. . 2018; 43 - 54.
IEEE	KARAGÜL M,AKTAŞ S,COŞKUN YILMAZ B,Yılmaz M,OREKİCİ TEMEL G "GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome." , ss.43 - 54, 2018.
ISNAD	KARAGÜL, Meryem İlkay vd. "GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome". (2018), 43-54.

APA	KARAGÜL M, AKTAŞ S, COŞKUN YILMAZ B, Yılmaz M, OREKİCİ TEMEL G (2018). GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome. Balkan Medical Journal, 35(1), 43 - 54.
Chicago	KARAGÜL Meryem İlkay,AKTAŞ SAVAŞ,COŞKUN YILMAZ BANU,Yılmaz Mustafa,OREKİCİ TEMEL GÜLHAN GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome. Balkan Medical Journal 35, no.1 (2018): 43 - 54.
MLA	KARAGÜL Meryem İlkay,AKTAŞ SAVAŞ,COŞKUN YILMAZ BANU,Yılmaz Mustafa,OREKİCİ TEMEL GÜLHAN GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome. Balkan Medical Journal, vol.35, no.1, 2018, ss.43 - 54.
AMA	KARAGÜL M,AKTAŞ S,COŞKUN YILMAZ B,Yılmaz M,OREKİCİ TEMEL G GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome. Balkan Medical Journal. 2018; 35(1): 43 - 54.
Vancouver	KARAGÜL M,AKTAŞ S,COŞKUN YILMAZ B,Yılmaz M,OREKİCİ TEMEL G GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome. Balkan Medical Journal. 2018; 35(1): 43 - 54.
IEEE	KARAGÜL M,AKTAŞ S,COŞKUN YILMAZ B,Yılmaz M,OREKİCİ TEMEL G "GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome." Balkan Medical Journal, 35, ss.43 - 54, 2018.
ISNAD	KARAGÜL, Meryem İlkay vd. "GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome". Balkan Medical Journal 35/1 (2018), 43-54.