Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?

Numan KARAARSLAN, (Namık Kemal University, Faculty of Medicine, Department of Neurosurgery, Tekirdağ, Turkey)
İbrahim YILMAZ, (Istanbul Medipol University Faculty of Medicine, Department of Medical Pharmacology, Istanbul, Turkey)
DUYGU YAŞAR ŞİRİN, (Namik Kemal University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, Tekirdag, Turkey)
Derya BAYKIZ, (Republic of Turkey, Ministry of Health, State Hospital, Department of Cardiology, Tekirdag, Turkey)
Aykut DEMİRKİRAN, (Republic of Turkey, Ministry of Health, State Hospital, Department of Cardiology, Tekirdag, Turkey)
Özkan ATEŞ (Istanbul Esenyurt University, Esencan Hospital, Department of Neurosurgery, Istanbul, Turkey)
Annals of Medical Research
6 2
Yıl: 2018 Cilt: 25 Sayı: 2 Sayfa Aralığı: 266 - 273 Metin Dili: İngilizce DOI: 10.5455/jtomc.2018.04.063 İndeks Tarihi: 27-03-2019

Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?

Öz:
Aim: Nimodipine is used to prevent cerebrovascular-originated ischemic neurological deficits, yet its effects on nucleus pulposus (NP) cells or annulus fibrosus (AF) cells weren’t studied. This study aimed to examine nimodipine’s effects on vitality and proliferation of chondroadherin (CHAD), type II collagen (COL2A1), and hypoxia-inducible factor 1 alpha (HIF 1α) gene expression in human primary NP/AF cells.Material and Methods: NP/AF cell cultures obtained from 6 patients who underwent microdiscectomy were treated with 100 µMolar nimodipine and analyzed at 0, 24, and 48 h. Data were evaluated using one-way ANOVA and post-hoc Tukey HSD with 95% confidence interval.Results: We observed suppressed cell proliferation and increased necrosis in nimodipine-treated NP/AF cell cultures, especially degenerated tissue. COL2A1 gene expression wasn’t detected in any experimental groups. CHAD and HIF 1α expression had timedependent decreases in control. CHAD and HIF 1α expression were found to decrease at 24h, but increased at 48h in degenerated tissue. In nimodipine-applied intact tissues, CHAD expression was stable at 24h but 1.62 times higher than control at 48h. HIF 1α levels were lower than control.Conclusion: In nimodipine-treated degenerated AF/NP cultures, CHAD and HIF 1α expressions had time-dependent decreases. However, after complete RT-PCR data evaluation, no correlation between nimodipine application and gene expression occurred.
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APA Karaarslan N, Yılmaz İ, YAŞAR ŞİRİN D, BAYKIZ D, DEMİRKİRAN A, ATEŞ Ö (2018). Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?. , 266 - 273. 10.5455/jtomc.2018.04.063
Chicago Karaarslan Numan,Yılmaz İbrahim,YAŞAR ŞİRİN DUYGU,BAYKIZ DERYA,DEMİRKİRAN Aykut,ATEŞ Özkan Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?. (2018): 266 - 273. 10.5455/jtomc.2018.04.063
MLA Karaarslan Numan,Yılmaz İbrahim,YAŞAR ŞİRİN DUYGU,BAYKIZ DERYA,DEMİRKİRAN Aykut,ATEŞ Özkan Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?. , 2018, ss.266 - 273. 10.5455/jtomc.2018.04.063
AMA Karaarslan N,Yılmaz İ,YAŞAR ŞİRİN D,BAYKIZ D,DEMİRKİRAN A,ATEŞ Ö Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?. . 2018; 266 - 273. 10.5455/jtomc.2018.04.063
Vancouver Karaarslan N,Yılmaz İ,YAŞAR ŞİRİN D,BAYKIZ D,DEMİRKİRAN A,ATEŞ Ö Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?. . 2018; 266 - 273. 10.5455/jtomc.2018.04.063
IEEE Karaarslan N,Yılmaz İ,YAŞAR ŞİRİN D,BAYKIZ D,DEMİRKİRAN A,ATEŞ Ö "Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?." , ss.266 - 273, 2018. 10.5455/jtomc.2018.04.063
ISNAD Karaarslan, Numan vd. "Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?". (2018), 266-273. https://doi.org/10.5455/jtomc.2018.04.063
APA Karaarslan N, Yılmaz İ, YAŞAR ŞİRİN D, BAYKIZ D, DEMİRKİRAN A, ATEŞ Ö (2018). Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?. Annals of Medical Research, 25(2), 266 - 273. 10.5455/jtomc.2018.04.063
Chicago Karaarslan Numan,Yılmaz İbrahim,YAŞAR ŞİRİN DUYGU,BAYKIZ DERYA,DEMİRKİRAN Aykut,ATEŞ Özkan Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?. Annals of Medical Research 25, no.2 (2018): 266 - 273. 10.5455/jtomc.2018.04.063
MLA Karaarslan Numan,Yılmaz İbrahim,YAŞAR ŞİRİN DUYGU,BAYKIZ DERYA,DEMİRKİRAN Aykut,ATEŞ Özkan Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?. Annals of Medical Research, vol.25, no.2, 2018, ss.266 - 273. 10.5455/jtomc.2018.04.063
AMA Karaarslan N,Yılmaz İ,YAŞAR ŞİRİN D,BAYKIZ D,DEMİRKİRAN A,ATEŞ Ö Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?. Annals of Medical Research. 2018; 25(2): 266 - 273. 10.5455/jtomc.2018.04.063
Vancouver Karaarslan N,Yılmaz İ,YAŞAR ŞİRİN D,BAYKIZ D,DEMİRKİRAN A,ATEŞ Ö Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?. Annals of Medical Research. 2018; 25(2): 266 - 273. 10.5455/jtomc.2018.04.063
IEEE Karaarslan N,Yılmaz İ,YAŞAR ŞİRİN D,BAYKIZ D,DEMİRKİRAN A,ATEŞ Ö "Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?." Annals of Medical Research, 25, ss.266 - 273, 2018. 10.5455/jtomc.2018.04.063
ISNAD Karaarslan, Numan vd. "Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?". Annals of Medical Research 25/2 (2018), 266-273. https://doi.org/10.5455/jtomc.2018.04.063
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23-04-2024 6:44

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