Determination of Organic Acids in Natural and Commercial Orange Juices by HPLC/DAD
Yıl: 2017 Cilt: 45 Sayı: 3 Sayfa Aralığı: 411 - 416 Metin Dili: İngilizce DOI: 10.15671/HJBC.2018.182 İndeks Tarihi: 07-11-2018
Determination of Organic Acids in Natural and Commercial Orange Juices by HPLC/DAD
Öz: This study aimed to develope a simple reversed-phase HPLC method for simultaneous determination of organic acids in natural and commercial orange juices. The chromatographic separation of these compounds was performed in a single run by using isocratic mobile phase consisting of 10 mM KH2PO4 (pH=2.2) at room temperature, with flow rate of 1 mL.min-1 within 10 min on Inertsil ODS-4 C18 column (250 x 4.0 mm, 5μm). An ultraviolet absorption at 210 nm and 245 nm was monitored. Linearity of calibration, accuracy, precision were examined as parts of the method validation. It has been found that the levels of organic acids in natural juices were higher than in commercial juices.
Anahtar Kelime: Konular:
Doğal ve Ticari Portakal Sularındaki Organik Asitlerin HPLC/DAD Yöntemiyle Tayini
Öz: Bu çalışma, doğal ve ticari portakal sularında, organik asitlerin eş zamanlı olarak tayini için basit bir ters faz HPLC yöntemi geliştirmeyi amaçlamıştır. Bileşiklerin kromatografik ayrımı izokratik olarak 10 mM KH2PO4 (pH= 2.2) hareketli fazıyla, oda sıcaklığında, 1 mL.min-1 akış hızında, 10 dakika içinde ve Inertsil ODS4 C18 kolonda (250 x 4.0 mm, 5μm), gerçekleşmiştir. Ultraviyole absorpsiyon 210 ve 245 nm dalgaboyunda izlenmiştir. Doğrusallık, doğruluk, kesinlik gibi parametreler belirlenerek yöntem valide edilmiştir. Doğal meyve sularındaki organik asit seviyesinin, ticari meyve sularından daha yüksek olduğu bulunmuştur.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | BÜYÜKTUNCEL S (2017). Determination of Organic Acids in Natural and Commercial Orange Juices by HPLC/DAD. , 411 - 416. 10.15671/HJBC.2018.182 |
| Chicago | BÜYÜKTUNCEL SALİHA EBRU Determination of Organic Acids in Natural and Commercial Orange Juices by HPLC/DAD. (2017): 411 - 416. 10.15671/HJBC.2018.182 |
| MLA | BÜYÜKTUNCEL SALİHA EBRU Determination of Organic Acids in Natural and Commercial Orange Juices by HPLC/DAD. , 2017, ss.411 - 416. 10.15671/HJBC.2018.182 |
| AMA | BÜYÜKTUNCEL S Determination of Organic Acids in Natural and Commercial Orange Juices by HPLC/DAD. . 2017; 411 - 416. 10.15671/HJBC.2018.182 |
| Vancouver | BÜYÜKTUNCEL S Determination of Organic Acids in Natural and Commercial Orange Juices by HPLC/DAD. . 2017; 411 - 416. 10.15671/HJBC.2018.182 |
| IEEE | BÜYÜKTUNCEL S "Determination of Organic Acids in Natural and Commercial Orange Juices by HPLC/DAD." , ss.411 - 416, 2017. 10.15671/HJBC.2018.182 |
| ISNAD | BÜYÜKTUNCEL, SALİHA EBRU. "Determination of Organic Acids in Natural and Commercial Orange Juices by HPLC/DAD". (2017), 411-416. https://doi.org/10.15671/HJBC.2018.182 |
| APA | BÜYÜKTUNCEL S (2017). Determination of Organic Acids in Natural and Commercial Orange Juices by HPLC/DAD. Hacettepe Journal of Biology and Chemistry, 45(3), 411 - 416. 10.15671/HJBC.2018.182 |
| Chicago | BÜYÜKTUNCEL SALİHA EBRU Determination of Organic Acids in Natural and Commercial Orange Juices by HPLC/DAD. Hacettepe Journal of Biology and Chemistry 45, no.3 (2017): 411 - 416. 10.15671/HJBC.2018.182 |
| MLA | BÜYÜKTUNCEL SALİHA EBRU Determination of Organic Acids in Natural and Commercial Orange Juices by HPLC/DAD. Hacettepe Journal of Biology and Chemistry, vol.45, no.3, 2017, ss.411 - 416. 10.15671/HJBC.2018.182 |
| AMA | BÜYÜKTUNCEL S Determination of Organic Acids in Natural and Commercial Orange Juices by HPLC/DAD. Hacettepe Journal of Biology and Chemistry. 2017; 45(3): 411 - 416. 10.15671/HJBC.2018.182 |
| Vancouver | BÜYÜKTUNCEL S Determination of Organic Acids in Natural and Commercial Orange Juices by HPLC/DAD. Hacettepe Journal of Biology and Chemistry. 2017; 45(3): 411 - 416. 10.15671/HJBC.2018.182 |
| IEEE | BÜYÜKTUNCEL S "Determination of Organic Acids in Natural and Commercial Orange Juices by HPLC/DAD." Hacettepe Journal of Biology and Chemistry, 45, ss.411 - 416, 2017. 10.15671/HJBC.2018.182 |
| ISNAD | BÜYÜKTUNCEL, SALİHA EBRU. "Determination of Organic Acids in Natural and Commercial Orange Juices by HPLC/DAD". Hacettepe Journal of Biology and Chemistry 45/3 (2017), 411-416. https://doi.org/10.15671/HJBC.2018.182 |
