Investigation the effects of cilostazol and rosuvastatin on kidney and heart: An experimental acute kidney and heart injury model

Dönmez, Köksal; KUŞKU, Fatmagül; Cakir, Habib; YUREKLI, ISMAIL; Eygi, Bortecin; GÜRBÜZ, ALİ; PEKER, İhsan; iscan, sahin

Investigation the effects of cilostazol and rosuvastatin on kidney and heart: An experimental acute kidney and heart injury model

Şahin İŞCAN, (İzmir Katip Çelebi Üniversitesi, Atatürk Eğitim ve Araştırma Hastanesi, Kalp ve Damar Cerrahisi Anabilim Dalı, İzmir, Türkiye)

Habib ÇAKIR, (İzmir Katip Çelebi Üniversitesi, Atatürk Eğitim ve Araştırma Hastanesi, Kalp ve Damar Cerrahisi Anabilim Dalı, İzmir, Türkiye)

Fatmagül KUŞKU, (İstanbul Bilim Üniversitesi, Patoloji Anabilim Dalı, İstanbul, Türkiye)

İsmail YÜREKLİ, (İzmir Katip Çelebi Üniversitesi, Atatürk Eğitim ve Araştırma Hastanesi, Kalp ve Damar Cerrahisi Anabilim Dalı, İzmir, Türkiye)

Börteçin EYGİ, (İzmir Katip Çelebi Üniversitesi, Atatürk Eğitim ve Araştırma Hastanesi, Kalp ve Damar Cerrahisi Anabilim Dalı, İzmir, Türkiye)

Köksal DÖNMEZ, (İzmir Katip Çelebi Üniversitesi, Atatürk Eğitim ve Araştırma Hastanesi, Kalp ve Damar Cerrahisi Anabilim Dalı, İzmir, Türkiye)

İhsan PEKER, (İzmir Katip Çelebi Üniversitesi, Atatürk Eğitim ve Araştırma Hastanesi, Kalp ve Damar Cerrahisi Anabilim Dalı, İzmir, Türkiye)

Ali GÜRBÜZ (İzmir Katip Çelebi Üniversitesi, Atatürk Eğitim ve Araştırma Hastanesi, Kalp ve Damar Cerrahisi Anabilim Dalı, İzmir, Türkiye)

Türk Göğüs Kalp Damar Cerrahisi Dergisi

3 1

Yıl: 2017 Cilt: 25 Sayı: 2 Sayfa Aralığı: 255 - 263 Metin Dili: İngilizce İndeks Tarihi: 09-11-2018

Investigation the effects of cilostazol and rosuvastatin on kidney and heart: An experimental acute kidney and heart injury model

Öz:

Background: In this study, we aimed to assess the effects of preoperative cilostazol and rosuvastatin therapy on kidney ischemia/ reperfusion injury and remote cardiac reperfusion injury in an experimental model.Methods: A total of 35 female Sprague-Dawley rats were randomly divided into five groups (n=7). Median laparotomy and a 45-min bilateral kidney ischemia were performed. Oral medications were administered three days before the surgical intervention (20 mg/kg cilostazol, 10 mg/kg rosuvastatin and 20 mg/kg cilostazol + 10 mg/kg rosuvastatin). Blood samples and kidney and heart tissue samples were extracted one day after surgery.Results: Immunohistochemical examination of the kidney samples revealed that tumor necrosis factor- alpha and hypoxia-inducible factor-1 alpha immunoreactivities in the cilostazol, rosuvastatin, and cilostazol + rosuvastatin groups were found to be signifiicantly lower, compared to ischemia/reperfusion injury group (p<0.05). Immunohistochemical examination of the heart samples revealed that tumor necrosis factor-alpha immunoreactivity was significantly lower in the cilostazol group, compared to ischemia/reperfusion injury group. Hypoxia-inducible factor-1 alpha immunoreactivities were significantly lower in the cilostazol, rosuvastatin, and cilostazol + rosuvastatin groups, compared to ischemia/reperfusion injury group (p<0.05). Serum urea, creatinine, creatine kinase-muscle and brain, and troponin levels were signifiicantly lower in the cilostazol, rosuvastatin, and cilostazol + rosuvastatin groups, compared to ischemia/reperfusion injury group (p<0.05).Conclusion: Cilostazol and rosuvastatin have protective effects on kidney ischemia/reperfusion and remote cardiac reperfusion injury, and the protective effect can be augmented with cilostazol monotherapy, compared to combined therapy.

Anahtar Kelime:

Konular: Kalp ve Kalp Damar Sistemi

Silostazol ve rosuvastatinin böbrek ve kalp üzerine etkilerinin araştırılması: Deneysel akut böbrek ve kalp hasarı modeli

Öz:

Amaç: Bu çalışmada, deneysel bir modelde ameliyat öncesi silostazol ve rosuvastatin tedavisinin böbrek iskemi/reperfüzyon hasarına olan etkisi ve uzak kalp reperfüzyon hasarı üzerine olan etkisi araştırıldı. Çalışma planı: Toplam 35 adet dişi Sprague-Dawley sıçan rastgele beş gruba ayrıldı (n=7). Median laparotomi yapılarak, her iki böbreğe 45 dakika süren iskemi uygulandı. Cerrahi girişimin üç gün öncesinden başlayarak oral tedavi uygulandı (20 mg/kg silostazol, 10 mg/kg rosuvastatin ve 20 mg/kg cilostazol + 10 mg/kg rosuvastatin). Cerrahi girişimden bir gün sonra ise kan örnekleri ve böbrek ve kalp doku örnekleri alındı. Bulgular: Böbrek dokularının immünhistokimyasal değerlendirmesinde tümör nekroz faktör-alfa ve hipoksi ile indüklenebilir faktör-1 alfa immünreaktivitesinin silostazol, rosuvastatin ve silostazol + rosuvastatin grubunda, iskemi/ reperfüzyon hasarı grubuna kıyasla anlamlı düzeyde daha düşük olduğu tespit edildi (p<0.05). Kalp dokularının immünhistokimyasal değerlendirmesinde tümör nekroz faktör-alfa immünreaktivitesi, iskemi/reperfüzyon hasarı grubuna kıyasla silostazol grubunda anlamlı düzeyde daha düşüktü. Hipoksi ile indüklenebilir faktör-1 alfa immünreaktivitesi, silostazol, rosuvastatin ve silostazol + rosuvastatin grubunda, iskemi/reperfüzyon hasarı grubuna kıyasla, anlamlı düzeyde daha düşüktü (p<0.05). İskemi/reperfüzyon hasarı grubuna kıyasla, silostazol, rosuvastatin ve silostazol + rosuvastatin grubunda, serum üre, kreatinin, kreatin kinaz-kas ve beyin ve troponin düzeyleri anlamlı düzeyde daha düşüktü (p<0.05). Sonuç: Silostazol ve rosuvastatinin böbrek iskemi/reperfüzyon ve uzak kardiyak reperfüzyon hasarı üzerinde koruyucu etkileri olmakla birlikte, bu etki kombine tedaviye kıyasla silostazol tedavisi ile artabilir.

Anahtar Kelime:

Konular: Kalp ve Kalp Damar Sistemi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	iscan s, Cakir H, KUŞKU F, YUREKLI I, Eygi B, Dönmez K, PEKER İ, GÜRBÜZ A (2017). Investigation the effects of cilostazol and rosuvastatin on kidney and heart: An experimental acute kidney and heart injury model. , 255 - 263.
Chicago	iscan sahin,Cakir Habib,KUŞKU Fatmagül,YUREKLI ISMAIL,Eygi Bortecin,Dönmez Köksal,PEKER İhsan,GÜRBÜZ ALİ Investigation the effects of cilostazol and rosuvastatin on kidney and heart: An experimental acute kidney and heart injury model. (2017): 255 - 263.
MLA	iscan sahin,Cakir Habib,KUŞKU Fatmagül,YUREKLI ISMAIL,Eygi Bortecin,Dönmez Köksal,PEKER İhsan,GÜRBÜZ ALİ Investigation the effects of cilostazol and rosuvastatin on kidney and heart: An experimental acute kidney and heart injury model. , 2017, ss.255 - 263.
AMA	iscan s,Cakir H,KUŞKU F,YUREKLI I,Eygi B,Dönmez K,PEKER İ,GÜRBÜZ A Investigation the effects of cilostazol and rosuvastatin on kidney and heart: An experimental acute kidney and heart injury model. . 2017; 255 - 263.
Vancouver	iscan s,Cakir H,KUŞKU F,YUREKLI I,Eygi B,Dönmez K,PEKER İ,GÜRBÜZ A Investigation the effects of cilostazol and rosuvastatin on kidney and heart: An experimental acute kidney and heart injury model. . 2017; 255 - 263.
IEEE	iscan s,Cakir H,KUŞKU F,YUREKLI I,Eygi B,Dönmez K,PEKER İ,GÜRBÜZ A "Investigation the effects of cilostazol and rosuvastatin on kidney and heart: An experimental acute kidney and heart injury model." , ss.255 - 263, 2017.
ISNAD	iscan, sahin vd. "Investigation the effects of cilostazol and rosuvastatin on kidney and heart: An experimental acute kidney and heart injury model". (2017), 255-263.

APA	iscan s, Cakir H, KUŞKU F, YUREKLI I, Eygi B, Dönmez K, PEKER İ, GÜRBÜZ A (2017). Investigation the effects of cilostazol and rosuvastatin on kidney and heart: An experimental acute kidney and heart injury model. Türk Göğüs Kalp Damar Cerrahisi Dergisi, 25(2), 255 - 263.
Chicago	iscan sahin,Cakir Habib,KUŞKU Fatmagül,YUREKLI ISMAIL,Eygi Bortecin,Dönmez Köksal,PEKER İhsan,GÜRBÜZ ALİ Investigation the effects of cilostazol and rosuvastatin on kidney and heart: An experimental acute kidney and heart injury model. Türk Göğüs Kalp Damar Cerrahisi Dergisi 25, no.2 (2017): 255 - 263.
MLA	iscan sahin,Cakir Habib,KUŞKU Fatmagül,YUREKLI ISMAIL,Eygi Bortecin,Dönmez Köksal,PEKER İhsan,GÜRBÜZ ALİ Investigation the effects of cilostazol and rosuvastatin on kidney and heart: An experimental acute kidney and heart injury model. Türk Göğüs Kalp Damar Cerrahisi Dergisi, vol.25, no.2, 2017, ss.255 - 263.
AMA	iscan s,Cakir H,KUŞKU F,YUREKLI I,Eygi B,Dönmez K,PEKER İ,GÜRBÜZ A Investigation the effects of cilostazol and rosuvastatin on kidney and heart: An experimental acute kidney and heart injury model. Türk Göğüs Kalp Damar Cerrahisi Dergisi. 2017; 25(2): 255 - 263.
Vancouver	iscan s,Cakir H,KUŞKU F,YUREKLI I,Eygi B,Dönmez K,PEKER İ,GÜRBÜZ A Investigation the effects of cilostazol and rosuvastatin on kidney and heart: An experimental acute kidney and heart injury model. Türk Göğüs Kalp Damar Cerrahisi Dergisi. 2017; 25(2): 255 - 263.
IEEE	iscan s,Cakir H,KUŞKU F,YUREKLI I,Eygi B,Dönmez K,PEKER İ,GÜRBÜZ A "Investigation the effects of cilostazol and rosuvastatin on kidney and heart: An experimental acute kidney and heart injury model." Türk Göğüs Kalp Damar Cerrahisi Dergisi, 25, ss.255 - 263, 2017.
ISNAD	iscan, sahin vd. "Investigation the effects of cilostazol and rosuvastatin on kidney and heart: An experimental acute kidney and heart injury model". Türk Göğüs Kalp Damar Cerrahisi Dergisi 25/2 (2017), 255-263.