Objectives: To evaluate corneal biomechanics before and after collagen crosslinking (CXL) in patients with progressive keratoconus.Materials and Methods: In this prospective study, CXL was performed under topical anesthesia after removal of the epithelium (epiofftechnique) by applying ultraviolet A (UVA) light at a wavelength of 365 nm and power of 3 mW/cm2 or 5.4 joule/cm2. Isoosmolar0.1% riboflavin solution was administered before and during UVA irradiation. In addition to ophthalmologic examination, ocularresponse analyzer measurements were performed pre- and postoperatively. Corneal hysteresis (CH), corneal resistance factor (CRF),corneal compensated intraocular pressure (IOPcc), Goldmann-correlated intraocular pressure (IOPg), and central corneal thickness (CCT)were recorded.Results: The study included 35 eyes of 30 patients with progressive keratoconus. The mean age was 28.2±6.5 years and postoperativefollow-up time was 20.2±14.7 months (range: 6-74 months). The mean CH was 8.60±1.23 mmHg preoperatively, 8.96±2.05 mmHg inthe early postoperative period (1-6 months), (p=0.28) and 8.96±1.28 mmHg in the late postoperative period (10-29 months) (p=0.48).Mean CRF was 7.13±1.50 mmHg preoperatively, 8.48±2.16 mmHg in the early postoperative period (p=0.009), and 7.71±1.29mmHg in the late postoperative period (p=0.40). Mean IOPcc was 12.78±2.34 mmHg preoperatively, 15.38±4.21 mmHg in the earlypostoperative period (p=0.12) and 13.68±3.61 mmHg in the late postoperative period (p=0.48). Mean IOPg was 9.56±2.73 mmHgpreoperatively, 13.01±4.45 mmHg in the early postoperative period (p=0.046), and 10.86±3.47 mmHg in the late postoperativeperiod (p=0.44). Mean CCT was 484.43±41.26 µm preoperatively, 474.16±64.74 µm in the early postoperative period (p=0.70), and470.38±33.64 µm in the late postoperative period (p=0.71).Conclusion: CXL is a treatment modality believed to affect corneal biomechanics in keratoconus, but the results of larger patient serieswith longer follow-up periods may enable a better evaluation.