TY  - JOUR
TI  - Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses
AB  - Objectives: To compare biofilm formations of two Staphylococcus epidermidis (S. epidermidis) isolates with known biofilm formationcapacities on four different intraocular lenses (IOL) that have not been studied before.Materials and Methods: Two isolates obtained from ocular surfaces and identified in previous studies and stored at -86 °C in 15%glycerol in the microbiology laboratory of the Anadolu University Department of Biology were purified and used in the study. The isolateswere S. epidermidis KA 15.8 (ICA+), a known biofilm producer isolate positive for icaA, icaD and bap genes, and S. epidermidis KA 14.5 (ICA-),known as a non-biofilm producer isolate negative for icaA, icaD and bap genes. The biofilm formation capacities of the 2 isolates on 4 differentIOLs were compared. Two of the IOLs were acrylic (UD613 [IOL A], Turkey; SA60AT [IOL B], USA), and the other two were polymethylmethacrylate (PMMA) (B60130C [IOL C], India; B55125C [IOL D], India). Bacterial enumeration and optical density measurements weredone from biofilms that formed on the IOLs. Biofilms were imaged using scanning electron microscopy.Results: Mean bacterial counts on the IOLs were 7.1±0.4 log10 CFU/mL with the ICA+ isolate, and 6.7±0.8 log10 CFU/mL with theICA- isolate; there were no statistically significant differences. Biofilm formation was lower with acrylic lenses than PMMA lenses withboth isolates (p=0.009 and p=0.013). The highest biofilm production was obtained on IOL C (PMMA) (p<0.001) and the lowest wasobtained on IOL A (hydrophilic acrylic) (p<0.001).Conclusion: Bacterial counts after biofilm formation were lower on acrylic lenses, especially hydrophilic acrylic with hydrophobicproperties. Further animal and in vivo studies are required to support the findings of this study.
AU  - ÖZMEN, Ahmet Tuncer
AU  - KIVANÇ, Merih
AU  - KILIÇ, VOLKAN
AU  - GÜLLÜLÜ, Gülay
AU  - Kıvanç, Sertaç Argun
DO  - 10.4274/tjo.79059
PY  - 2017
JO  - Türk Oftalmoloji Dergisi
VL  - 47
IS  - 2
SN  - 2147-2661
SP  - 68
EP  - 73
DB  - TRDizin
UR  - http://search/yayin/detay/288453
ER  -