TY  - JOUR
TI  - Screening of antimicrobial, cytotoxic effects and phenolic compounds of the moss Dicranum scoparium
AB  - Bryophytes belong to the group of the oldest known land plants, which includes liverworts, hornworts, and mosses. Recent research shows that mosses contain remarkable and unique substances with high biological activity. This study describes phenolic compounds, antimicrobial and cytotoxic activities of Dicranum scoparium (Hedw.) collected from Sundiken Mountains, Eskisehir. Total phenols contents of the extracts were determined by FolinCiocalteu assay and their amount ranged from 81.90 0.10 to 105.21± 0.10 mg/g of gallic acid equivalents. The highest total phenolic content were obtained from the ethyl acetate extract (extract C). Phenolic acids in the extracts acquired by using different polarity solvents were separated by reverse-phase High-Performance Liquid Chromatography, which enabled advanced separation by the use of a C18 column, an acidic mobile phase, and gradient elusion. Also, the most phenolic acid content was measured in the ethyl acetate extract. The results revealed that all extracts were active against Pseudomonas aeruginosa and Bacillus subtilis. Acetone extract and extract C had a potential against 4 tested bacteria. Extract A demonstrated inhibition against 3 bacteria and 3 fungi. The minimum inhibitory concentrations of the most effective extracts ranged from 11.7-187.5 µg/mL for the bacterial strains. Furthermore, methanol, B and C extracts at 0.17, 1.7, 17, 85 and 170 µg/mL concentrations were tested on rat glioma (C6) cells. Although none of the above methanol doses affected in 24 hours, 85 and 170 µg/mL slightly decreased C6 viability in 48 hours. Extract B at doses of 17, 85 and 170 µg/mL decreased C6 survival dose dependently in 24 and 48 hours. Only 170 µg/mL extract C reduced the survival ratio in 24 hours, but in 48 hours 0.17, 1.7, 17, 85 and 170 µg/mL concentrations inhibited cell viability. The present study suggests the possibility that D. scoparium may possess antimicrobial and anticancer molecule(s).
AU  - SAVAROĞLU, Filiz
AU  - ÖZTOPCU VATAN, AYŞE PINAR
AU  - kabadere, selda
AU  - Öztürk, Nilgün
AU  - İlhan, Semra
AU  - FİLİK İŞÇEN, Cansu
PY  - 2018
JO  - Biological Diversity and Conservation
VL  - 11
IS  - 1
SN  - 1308-5301
SP  - 87
EP  - 94
DB  - TRDizin
UR  - http://search/yayin/detay/288774
ER  -