FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO

DÜZYER GEBİZLİ, ŞEBNEM

FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO

ŞEBNEM DÜZYER GEBİZLİ (Department of Textile Engineering, Uludag University, Bursa, Turkey)

Tekstil ve Konfeksiyon

2 0

Yıl: 2017 Cilt: 27 Sayı: 4 Sayfa Aralığı: 334 - 341 Metin Dili: İngilizce İndeks Tarihi: 24-12-2018

FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO

Öz:

Fiber diameter and fiber mat porosity play a key role on cell adhesion and proliferation. Therefore, in this study, poly(ethyleneterephthalate) (PET) scaffolds consisting of fibers with different diameters and pore sizes were fabricated from solutions with differentconcentrations (10, 15 and 20% wt.) by electrospinning. Also, the effect of fiber mat thickness was investigated. The scaffolds werecharacterized in terms of surface and mechanical properties. The electrospun fibers had diameters ranging from 0.575 to 2.825 µmdepending on the polymer concentration. Contact angle values showed that PET scaffolds had super hydrophobic structure. Aftercharacterization, fibroblast cells were cultured onto PET scaffolds and influence of different electrospinning parameters on cellproliferation was discussed. Fibroblast cells showed better proliferation on scaffolds consisting of smaller diameters. After evaluation offibroblast cell proliferation on electrospun PET scaffolds, two different electrospun scaffolds were chosen: 1) scaffold with the smallestfiber diameter and 2) scaffold with better mechanical properties. Endothelial and keratinocyte cells were cultured on those twoelectrospun scaffolds. Cell adhesion and proliferation behavior of endothelial and keratinocyte cells on those scaffolds wereinvestigated.

Anahtar Kelime:

Konular: Malzeme Bilimleri, Tekstil

ELEKTRO ÇEKİM YÖNTEMİ İLE POLİETİLEN TEREFTALAT DOKU İSKELESİ ÜRETİMİ: KARAKTERİZASYONU VE IN VITRO ORTAMDA HÜCRE ÇOĞALMASINDAKİ POTANSİYELİ

Öz:

Lif çapı ve yüzey gözenekliliği hücre tutunması ve çoğalmasında önemli bir rol oynamaktadır. Bu nedenle bu çalışmada, farklı lif çaplarına ve yüzey gözenekliliğine sahip polietilen tereftalat (PET) doku iskeleleri elektro çekim yöntemi ile farklı PET konsantrasyonuna sahip çözeltilerden (ağırlıkça %10,%15 ve %20) üretilmiştir. Ayrıca, üretilen yüzeylerin kalınlığının etkisi incelenmiştir. Doku iskelelerinin yüzeysel ve mekaniksel karakterizasyonu yapılmıştır. Elektro çekim yöntemi ile üretilen liflerin polimer konsantrasyonuna bağlı olarak 0.575 µm’den 2.825 µm’ye değişen aralıkta çaplara sahip olduğu görülmüştür. Temas açısı ölçümleri PET doku iskelelerinin süper hidrofobik yapıya sahip olduğunu göstermiştir. Karakterizasyon sonrası, PET doku iskeleleri üzerine fibroblast hücre ekimleri yapılmış ve farklı elektro çekim parametrelerinin hücre çoğalması üzerine etkisi araştırılmıştır. Fibroblast hücreleri küçük çaplara sahip liflerden oluşan yüzeyler üzerinde daha iyi çoğalmıştır. PET doku iskeleleri üzerine fibroblast hücre çoğalması davranışı incelendikten sonra, üretilmiş olan dokuz yüzeyden en küçük lif çapına sahip olan ve daha iyi mekanik özelliklere sahip olan iki yüzey seçilmiştir. Bu iki yüzey üzerine endotel ve keratinosit hücre ekimleri yapılmıştır. Endotel ve keratinosit hücrelerinin bu iki yüzey üzerindeki hücre tutunması ve çoğalması davranışları incelenmiştir.

Anahtar Kelime:

Konular: Malzeme Bilimleri, Tekstil

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	DÜZYER GEBİZLİ Ş (2017). FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO. , 334 - 341.
Chicago	DÜZYER GEBİZLİ ŞEBNEM FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO. (2017): 334 - 341.
MLA	DÜZYER GEBİZLİ ŞEBNEM FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO. , 2017, ss.334 - 341.
AMA	DÜZYER GEBİZLİ Ş FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO. . 2017; 334 - 341.
Vancouver	DÜZYER GEBİZLİ Ş FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO. . 2017; 334 - 341.
IEEE	DÜZYER GEBİZLİ Ş "FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO." , ss.334 - 341, 2017.
ISNAD	DÜZYER GEBİZLİ, ŞEBNEM. "FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO". (2017), 334-341.

APA	DÜZYER GEBİZLİ Ş (2017). FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO. Tekstil ve Konfeksiyon, 27(4), 334 - 341.
Chicago	DÜZYER GEBİZLİ ŞEBNEM FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO. Tekstil ve Konfeksiyon 27, no.4 (2017): 334 - 341.
MLA	DÜZYER GEBİZLİ ŞEBNEM FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO. Tekstil ve Konfeksiyon, vol.27, no.4, 2017, ss.334 - 341.
AMA	DÜZYER GEBİZLİ Ş FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO. Tekstil ve Konfeksiyon. 2017; 27(4): 334 - 341.
Vancouver	DÜZYER GEBİZLİ Ş FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO. Tekstil ve Konfeksiyon. 2017; 27(4): 334 - 341.
IEEE	DÜZYER GEBİZLİ Ş "FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO." Tekstil ve Konfeksiyon, 27, ss.334 - 341, 2017.
ISNAD	DÜZYER GEBİZLİ, ŞEBNEM. "FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO". Tekstil ve Konfeksiyon 27/4 (2017), 334-341.