Identification of root-knot nematodes in the Mediterranean Region of Turkey by using rDNA and mtDNA markers

ELEKÇİOĞLU, İ. Halil; Gözel, Uğur; DEVRAN, Zübeyir; YILDIZ, ŞENOL; SÖĞÜT, M. Ali

Identification of root-knot nematodes in the Mediterranean Region of Turkey by using rDNA and mtDNA markers

Zübeyir DEVRAN, (Çukurova Üniversitesi, Narenciye ve Seracılık Araştırma Enstitüsü, Antalya, Türkiye)

Uğur GÖZEL, (Girilmedi)

M. Ali SÖĞÜT, (Girilmedi)

Şenol YILDIZ, (Girilmedi)

İ. Halil ELEKÇİOĞLU (Girilmedi)

Turkish Journal of Agriculture and Forestry

1 0

Yıl: 2002 Cilt: 26 Sayı: 6 Sayfa Aralığı: 337 - 341 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Identification of root-knot nematodes in the Mediterranean Region of Turkey by using rDNA and mtDNA markers

Öz:

Fragments containing the internal transcribed spacer (ITS) ribosomal rDNA were amplified from Meloidogyne incognita, M. javanica, M. arenaria and M. hapla by polymerase chain reaction (PCR). Only digestion of the ITS region with the enzyme RsaI significantly distinguished M. hapla from other species, while other enzymes, EcoRI and BamHI, were not effective in distinguishing these nematode species. PCR was also done with mitocondrial DNA (mtDNA) markers for species discrimination. The obtained amplification product of 600 bp was digested with HinfI restriction endonuclease allowing discrimination among the four root-knot species. mtDNA was found to be a useful tool for discriminating between the important Meloidogyne species in this study.

Anahtar Kelime: nematodes mitochondrial DNA Meloidogyne javanica Nematoda Meloidogyne hapla Meloidogyne polymerase chain reaction genetic markers Meloidogyne arenaria ribosomal DNA Mediterranean Region identification

Konular: Orman Mühendisliği

Türkiye'nin Akdeniz Bölgesindeki kök ur nematodlarının rDNA ve mtDNA markerleri ile tanımlanması

Öz:

Anahtar Kelime: genetik belirleyiciler Meloidogyne arenaria ribozomal DNA Akdeniz Bölgesi tanı nematod mitokondriyal DNA Meloidogyne javanica iplik solucanları Meloidogyne hapla Meloidogyne polimeraz zincir reaksiyonu

Konular: Orman Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	DEVRAN Z, Gözel U, SÖĞÜT M, YILDIZ Ş, ELEKÇİOĞLU İ (2002). Identification of root-knot nematodes in the Mediterranean Region of Turkey by using rDNA and mtDNA markers. , 337 - 341.
Chicago	DEVRAN Zübeyir,Gözel Uğur,SÖĞÜT M. Ali,YILDIZ ŞENOL,ELEKÇİOĞLU İ. Halil Identification of root-knot nematodes in the Mediterranean Region of Turkey by using rDNA and mtDNA markers. (2002): 337 - 341.
MLA	DEVRAN Zübeyir,Gözel Uğur,SÖĞÜT M. Ali,YILDIZ ŞENOL,ELEKÇİOĞLU İ. Halil Identification of root-knot nematodes in the Mediterranean Region of Turkey by using rDNA and mtDNA markers. , 2002, ss.337 - 341.
AMA	DEVRAN Z,Gözel U,SÖĞÜT M,YILDIZ Ş,ELEKÇİOĞLU İ Identification of root-knot nematodes in the Mediterranean Region of Turkey by using rDNA and mtDNA markers. . 2002; 337 - 341.
Vancouver	DEVRAN Z,Gözel U,SÖĞÜT M,YILDIZ Ş,ELEKÇİOĞLU İ Identification of root-knot nematodes in the Mediterranean Region of Turkey by using rDNA and mtDNA markers. . 2002; 337 - 341.
IEEE	DEVRAN Z,Gözel U,SÖĞÜT M,YILDIZ Ş,ELEKÇİOĞLU İ "Identification of root-knot nematodes in the Mediterranean Region of Turkey by using rDNA and mtDNA markers." , ss.337 - 341, 2002.
ISNAD	DEVRAN, Zübeyir vd. "Identification of root-knot nematodes in the Mediterranean Region of Turkey by using rDNA and mtDNA markers". (2002), 337-341.

APA	DEVRAN Z, Gözel U, SÖĞÜT M, YILDIZ Ş, ELEKÇİOĞLU İ (2002). Identification of root-knot nematodes in the Mediterranean Region of Turkey by using rDNA and mtDNA markers. Turkish Journal of Agriculture and Forestry, 26(6), 337 - 341.
Chicago	DEVRAN Zübeyir,Gözel Uğur,SÖĞÜT M. Ali,YILDIZ ŞENOL,ELEKÇİOĞLU İ. Halil Identification of root-knot nematodes in the Mediterranean Region of Turkey by using rDNA and mtDNA markers. Turkish Journal of Agriculture and Forestry 26, no.6 (2002): 337 - 341.
MLA	DEVRAN Zübeyir,Gözel Uğur,SÖĞÜT M. Ali,YILDIZ ŞENOL,ELEKÇİOĞLU İ. Halil Identification of root-knot nematodes in the Mediterranean Region of Turkey by using rDNA and mtDNA markers. Turkish Journal of Agriculture and Forestry, vol.26, no.6, 2002, ss.337 - 341.
AMA	DEVRAN Z,Gözel U,SÖĞÜT M,YILDIZ Ş,ELEKÇİOĞLU İ Identification of root-knot nematodes in the Mediterranean Region of Turkey by using rDNA and mtDNA markers. Turkish Journal of Agriculture and Forestry. 2002; 26(6): 337 - 341.
Vancouver	DEVRAN Z,Gözel U,SÖĞÜT M,YILDIZ Ş,ELEKÇİOĞLU İ Identification of root-knot nematodes in the Mediterranean Region of Turkey by using rDNA and mtDNA markers. Turkish Journal of Agriculture and Forestry. 2002; 26(6): 337 - 341.
IEEE	DEVRAN Z,Gözel U,SÖĞÜT M,YILDIZ Ş,ELEKÇİOĞLU İ "Identification of root-knot nematodes in the Mediterranean Region of Turkey by using rDNA and mtDNA markers." Turkish Journal of Agriculture and Forestry, 26, ss.337 - 341, 2002.
ISNAD	DEVRAN, Zübeyir vd. "Identification of root-knot nematodes in the Mediterranean Region of Turkey by using rDNA and mtDNA markers". Turkish Journal of Agriculture and Forestry 26/6 (2002), 337-341.