Yıl: 2002 Cilt: 26 Sayı: 3 Sayfa Aralığı: 163 - 170 Metin Dili: Türkçe

Protoplast fusion in sugar beet (Beta vulgaris L.)

Öz:
Bu araştırmada, daha önceki denemelerimizden elde edilen .BM315 adlı şeker pancarı ıslah hattına ait süspansiyon-kökenli protoplastlar ile yine üç şeker pancarı ıslah hattına (M114, ELK345 ve M1017) ait mezofil-kökenli protoplastların füzyonuna çalışılmıştır. üç farklı PEG (%20, %25 ve %30) ile üç farklı uygulama süresinin (15, 20 veya 25 dakika) değişik kombinasyonları denenmiştir. Füzyon etkinliği; canlılık, hetereokaryon sıklığı ve füzyona uğrayan protoplastların PE (plating efficiency) değerlerinin dört haftalık kültürden sonra ölçülmesi ile belirlenmiştir. Genel olarak, %25 PEG ile 20 dakikalık uygulama süresinin kombinasyonunda en yüksek füzyon sıklığı elde edilmiş fakat en yüksek canlılık oranı %20 PEG ile 25 dakikalık uygulama süresi sonunda elde edilmiştir. Hetereokaryonların hücre kolonisi oluşturma kapasitesi, düşük PEG düzeylerinin (%20 veya %25) kısa süreli uygulamalarla (15 veya 20 dakika) kombine edilmesi halinde daha yüksek olmuştur. Füzyona uğrayan protoplastlardan makrokallus veya bitki rejenerasyonu ise elde edilememiştir.
Anahtar Kelime: polietilen glikol Beta vulgaris hatlar şeker pancarı ıslah protoplast füzyonu canlılık

Şeker pancarında (Beta vulgaris L.) protoplast füzyonu

Öz:
In this study, attempts were made to fuse the suspension-derived protoplasts of a sugar beet breeding line (ÇBM315) with the mesophyll-derived protoplasts of three breeding lines (M114, ELK345 and M1017), which were obtained from our previous experiments. Combinations of three different PEG concentrations (20%, 25% or 30%) and three different treatment durations (15, 20 or 25 min) were tested. The fusion efficiency was determined by measuring the viability, heterokaryon frequency and plating efficiency (PE) of the fused protoplasts after four weeks of culture. In general, 25% PEG combined with 20 min treatment duration produced the highest fusion frequency, but the highest rate of viability was obtained at 20% PEG with 25 min treatment duration. The capacity of heterokaryons to form cell colonies was highest when lower PEG (20 or 25%) was used for shorter treatment durations (15 or 20 min). No macrocallus or regeneration from the fused protoplasts was achieved
Anahtar Kelime: sugarbeet viability protoplast fusion Beta vulgaris lines polyethylene glycol breeding

Belge Türü: Makale Makale Türü: Diğer Erişim Türü: Erişime Açık
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APA GÜREL S, GÜREL E, KAYA Z (2002). Protoplast fusion in sugar beet (Beta vulgaris L.). , 163 - 170.
Chicago GÜREL SONGÜL,GÜREL EKREM,KAYA ZEKİ Protoplast fusion in sugar beet (Beta vulgaris L.). (2002): 163 - 170.
MLA GÜREL SONGÜL,GÜREL EKREM,KAYA ZEKİ Protoplast fusion in sugar beet (Beta vulgaris L.). , 2002, ss.163 - 170.
AMA GÜREL S,GÜREL E,KAYA Z Protoplast fusion in sugar beet (Beta vulgaris L.). . 2002; 163 - 170.
Vancouver GÜREL S,GÜREL E,KAYA Z Protoplast fusion in sugar beet (Beta vulgaris L.). . 2002; 163 - 170.
IEEE GÜREL S,GÜREL E,KAYA Z "Protoplast fusion in sugar beet (Beta vulgaris L.)." , ss.163 - 170, 2002.
ISNAD GÜREL, SONGÜL vd. "Protoplast fusion in sugar beet (Beta vulgaris L.)". (2002), 163-170.
APA GÜREL S, GÜREL E, KAYA Z (2002). Protoplast fusion in sugar beet (Beta vulgaris L.). Turkish Journal of Biology, 26(3), 163 - 170.
Chicago GÜREL SONGÜL,GÜREL EKREM,KAYA ZEKİ Protoplast fusion in sugar beet (Beta vulgaris L.). Turkish Journal of Biology 26, no.3 (2002): 163 - 170.
MLA GÜREL SONGÜL,GÜREL EKREM,KAYA ZEKİ Protoplast fusion in sugar beet (Beta vulgaris L.). Turkish Journal of Biology, vol.26, no.3, 2002, ss.163 - 170.
AMA GÜREL S,GÜREL E,KAYA Z Protoplast fusion in sugar beet (Beta vulgaris L.). Turkish Journal of Biology. 2002; 26(3): 163 - 170.
Vancouver GÜREL S,GÜREL E,KAYA Z Protoplast fusion in sugar beet (Beta vulgaris L.). Turkish Journal of Biology. 2002; 26(3): 163 - 170.
IEEE GÜREL S,GÜREL E,KAYA Z "Protoplast fusion in sugar beet (Beta vulgaris L.)." Turkish Journal of Biology, 26, ss.163 - 170, 2002.
ISNAD GÜREL, SONGÜL vd. "Protoplast fusion in sugar beet (Beta vulgaris L.)". Turkish Journal of Biology 26/3 (2002), 163-170.