Yıl: 2002 Cilt: 26 Sayı: 5 Sayfa Aralığı: 1201 - 1203 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog

Öz:
In this study, the detection of canine parvovirus (CPV) in a fecal sample from a dog with enteritis was performed for the first time using the polymerase chain reaction (PCR) in Turkey. The final PCR product was analyzed using the restriction fragment length polymorphysm (RFLP) technique. RFLP analysis using Apa LI and Eco RV restriction endonucleases revealed homology in the nucleotide sequence in at least the VP2 coding region of the virus DNAs detected in the fecal specimen and prepared from attenuated vaccine virus as a positive control.
Anahtar Kelime: dogs canine parvovirus polymerase chain reaction diagnosis DNA

Konular: Ziraat Mühendisliği

Bir köpekte canine parvovirus DNA' sının polimeraz zincir reaksiyonu (PZR) ile tespiti ve RFLP analizi

Öz:
In this study, the detection of canine parvovirus (CPV) in a fecal sample from a dog with enteritis was performed for the first time using the polymerase chain reaction (PCR) in Turkey. The final PCR product was analyzed using the restriction fragment length polymorphysm (RFLP) technique. RFLP analysis using Apa LI and Eco RV restriction endonucleases revealed homology in the nucleotide sequence in at least the VP2 coding region of the virus DNAs detected in the fecal specimen and prepared from attenuated vaccine virus as a positive control.
Anahtar Kelime: teşhis DNA köpek köpek parvovirüsü polimeraz zincir reaksiyonu

Konular: Ziraat Mühendisliği
Belge Türü: Makale Makale Türü: Diğer Erişim Türü: Erişime Açık
APA Ozkul A, KELES I, KARAOĞLU M, ÇABALAR M, BURGU İ (2002). Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. , 1201 - 1203.
Chicago Ozkul Aykut,KELES IHSAN,KARAOĞLU Mehmet Taner,ÇABALAR Mehmet,BURGU İbrahim Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. (2002): 1201 - 1203.
MLA Ozkul Aykut,KELES IHSAN,KARAOĞLU Mehmet Taner,ÇABALAR Mehmet,BURGU İbrahim Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. , 2002, ss.1201 - 1203.
AMA Ozkul A,KELES I,KARAOĞLU M,ÇABALAR M,BURGU İ Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. . 2002; 1201 - 1203.
Vancouver Ozkul A,KELES I,KARAOĞLU M,ÇABALAR M,BURGU İ Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. . 2002; 1201 - 1203.
IEEE Ozkul A,KELES I,KARAOĞLU M,ÇABALAR M,BURGU İ "Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog." , ss.1201 - 1203, 2002.
ISNAD Ozkul, Aykut vd. "Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog". (2002), 1201-1203.
APA Ozkul A, KELES I, KARAOĞLU M, ÇABALAR M, BURGU İ (2002). Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. Turkish Journal of Veterinary and Animal Sciences, 26(5), 1201 - 1203.
Chicago Ozkul Aykut,KELES IHSAN,KARAOĞLU Mehmet Taner,ÇABALAR Mehmet,BURGU İbrahim Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. Turkish Journal of Veterinary and Animal Sciences 26, no.5 (2002): 1201 - 1203.
MLA Ozkul Aykut,KELES IHSAN,KARAOĞLU Mehmet Taner,ÇABALAR Mehmet,BURGU İbrahim Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. Turkish Journal of Veterinary and Animal Sciences, vol.26, no.5, 2002, ss.1201 - 1203.
AMA Ozkul A,KELES I,KARAOĞLU M,ÇABALAR M,BURGU İ Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. Turkish Journal of Veterinary and Animal Sciences. 2002; 26(5): 1201 - 1203.
Vancouver Ozkul A,KELES I,KARAOĞLU M,ÇABALAR M,BURGU İ Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. Turkish Journal of Veterinary and Animal Sciences. 2002; 26(5): 1201 - 1203.
IEEE Ozkul A,KELES I,KARAOĞLU M,ÇABALAR M,BURGU İ "Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog." Turkish Journal of Veterinary and Animal Sciences, 26, ss.1201 - 1203, 2002.
ISNAD Ozkul, Aykut vd. "Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog". Turkish Journal of Veterinary and Animal Sciences 26/5 (2002), 1201-1203.