Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog
Yıl: 2002 Cilt: 26 Sayı: 5 Sayfa Aralığı: 1201 - 1203 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022
Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog
Öz: In this study, the detection of canine parvovirus (CPV) in a fecal sample from a dog with enteritis was performed for the first time using the polymerase chain reaction (PCR) in Turkey. The final PCR product was analyzed using the restriction fragment length polymorphysm (RFLP) technique. RFLP analysis using Apa LI and Eco RV restriction endonucleases revealed homology in the nucleotide sequence in at least the VP2 coding region of the virus DNAs detected in the fecal specimen and prepared from attenuated vaccine virus as a positive control.
Anahtar Kelime: Konular:
Bir köpekte canine parvovirus DNA' sının polimeraz zincir reaksiyonu (PZR) ile tespiti ve RFLP analizi
Öz: In this study, the detection of canine parvovirus (CPV) in a fecal sample from a dog with enteritis was performed for the first time using the polymerase chain reaction (PCR) in Turkey. The final PCR product was analyzed using the restriction fragment length polymorphysm (RFLP) technique. RFLP analysis using Apa LI and Eco RV restriction endonucleases revealed homology in the nucleotide sequence in at least the VP2 coding region of the virus DNAs detected in the fecal specimen and prepared from attenuated vaccine virus as a positive control.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Diğer Erişim Türü: Erişime Açık
APA | Ozkul A, KELES I, KARAOĞLU M, ÇABALAR M, BURGU İ (2002). Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. , 1201 - 1203. |
Chicago | Ozkul Aykut,KELES IHSAN,KARAOĞLU Mehmet Taner,ÇABALAR Mehmet,BURGU İbrahim Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. (2002): 1201 - 1203. |
MLA | Ozkul Aykut,KELES IHSAN,KARAOĞLU Mehmet Taner,ÇABALAR Mehmet,BURGU İbrahim Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. , 2002, ss.1201 - 1203. |
AMA | Ozkul A,KELES I,KARAOĞLU M,ÇABALAR M,BURGU İ Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. . 2002; 1201 - 1203. |
Vancouver | Ozkul A,KELES I,KARAOĞLU M,ÇABALAR M,BURGU İ Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. . 2002; 1201 - 1203. |
IEEE | Ozkul A,KELES I,KARAOĞLU M,ÇABALAR M,BURGU İ "Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog." , ss.1201 - 1203, 2002. |
ISNAD | Ozkul, Aykut vd. "Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog". (2002), 1201-1203. |
APA | Ozkul A, KELES I, KARAOĞLU M, ÇABALAR M, BURGU İ (2002). Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. Turkish Journal of Veterinary and Animal Sciences, 26(5), 1201 - 1203. |
Chicago | Ozkul Aykut,KELES IHSAN,KARAOĞLU Mehmet Taner,ÇABALAR Mehmet,BURGU İbrahim Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. Turkish Journal of Veterinary and Animal Sciences 26, no.5 (2002): 1201 - 1203. |
MLA | Ozkul Aykut,KELES IHSAN,KARAOĞLU Mehmet Taner,ÇABALAR Mehmet,BURGU İbrahim Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. Turkish Journal of Veterinary and Animal Sciences, vol.26, no.5, 2002, ss.1201 - 1203. |
AMA | Ozkul A,KELES I,KARAOĞLU M,ÇABALAR M,BURGU İ Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. Turkish Journal of Veterinary and Animal Sciences. 2002; 26(5): 1201 - 1203. |
Vancouver | Ozkul A,KELES I,KARAOĞLU M,ÇABALAR M,BURGU İ Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog. Turkish Journal of Veterinary and Animal Sciences. 2002; 26(5): 1201 - 1203. |
IEEE | Ozkul A,KELES I,KARAOĞLU M,ÇABALAR M,BURGU İ "Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog." Turkish Journal of Veterinary and Animal Sciences, 26, ss.1201 - 1203, 2002. |
ISNAD | Ozkul, Aykut vd. "Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog". Turkish Journal of Veterinary and Animal Sciences 26/5 (2002), 1201-1203. |