An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by Using Reversed Phase Liquid Chromatography Method
Yıl: 2017 Cilt: 7 Sayı: 2 Sayfa Aralığı: 602 - 607 Metin Dili: İngilizce DOI: 10.7212/zkufbd.v7i2.589 İndeks Tarihi: 03-04-2019
An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by Using Reversed Phase Liquid Chromatography Method
Öz: Pravastatin belongs to the group of anti-cholestrol agents used in the treatment of hypercholesterolemia. Reversed phase liquidchromatographic (RPLC) method was described in this study for its determination from tablet formulation. This method wasimproved with a slight change in the pH of the mobile phase. The experiment was carried out a C18 column with a mobile phase ofa binary mixture of acetonitrile and water (40:60, v/v) delivered at a flow rate of 1 mL/min and detection was carried out at 239 nm.The linear range was found as 2 - 12 µg/mL. The limits of detection (LOD) and quantitation (LOQ) for pravastatin were found to be0.260 ug/mL and 0.787 ug/mL, respectively. The optimization results, together with statistical evaluation of the data, demonstratedthe reliability of this method.
Anahtar Kelime: Ters Faz Sıvı Kromatografi Yöntemiyle İlaç Formülasyonunda Pravastatin Tayini İçin Geliştirilmiş Optimizasyon Çalışması
Öz: Pravastatin, anti-kolesterol ajanlar grubuna dahil olan ve yüksek kolesterol tedavisinde kullanılan bir ilaçtır. Bu çalışmada pravastatinin tablet formulasyonundan tayini için ters faz sıvı kromatografi (RPLC) metodu tanımlanmıştır. Önerilen metot mobil faz pH’sında küçük değişiklik yapılarak geliştirilmiştir. Kromatografik ayrım C18 kolon kullanılarak, asetonitril-su (40:60, v/v) ikili mobil faz karışımında gerçekleştirilmiştir. Akış hızının 1 mL/dakika olduğu çalışma 239 nm’de yürütülmüştür. Çalışmada lineer aralık 2-12 µg/mL olarak belirlenmiştir. Pravastatin için dedeksiyon limiti (LOD) ve kantitatif yorum limiti (LOQ) değerleri sırasıyla 0.260 µg/ mL ve 0.787 µg/mL olarak olarak ölçülmüştür. Optimizasyon sonuçları, istatistiksel verilerle birlikte değerlendirildiğinde metodun güvenilirliğini ortaya koymaktadır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Üstün Z, DALDAL Y, TALAY AYDOĞAN A, ÇUBUK DEMİRALAY E (2017). An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by Using Reversed Phase Liquid Chromatography Method. , 602 - 607. 10.7212/zkufbd.v7i2.589 |
| Chicago | Üstün Zehra,DALDAL Y. Doğan,TALAY AYDOĞAN Aybike,ÇUBUK DEMİRALAY Ebru An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by Using Reversed Phase Liquid Chromatography Method. (2017): 602 - 607. 10.7212/zkufbd.v7i2.589 |
| MLA | Üstün Zehra,DALDAL Y. Doğan,TALAY AYDOĞAN Aybike,ÇUBUK DEMİRALAY Ebru An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by Using Reversed Phase Liquid Chromatography Method. , 2017, ss.602 - 607. 10.7212/zkufbd.v7i2.589 |
| AMA | Üstün Z,DALDAL Y,TALAY AYDOĞAN A,ÇUBUK DEMİRALAY E An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by Using Reversed Phase Liquid Chromatography Method. . 2017; 602 - 607. 10.7212/zkufbd.v7i2.589 |
| Vancouver | Üstün Z,DALDAL Y,TALAY AYDOĞAN A,ÇUBUK DEMİRALAY E An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by Using Reversed Phase Liquid Chromatography Method. . 2017; 602 - 607. 10.7212/zkufbd.v7i2.589 |
| IEEE | Üstün Z,DALDAL Y,TALAY AYDOĞAN A,ÇUBUK DEMİRALAY E "An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by Using Reversed Phase Liquid Chromatography Method." , ss.602 - 607, 2017. 10.7212/zkufbd.v7i2.589 |
| ISNAD | Üstün, Zehra vd. "An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by Using Reversed Phase Liquid Chromatography Method". (2017), 602-607. https://doi.org/10.7212/zkufbd.v7i2.589 |
| APA | Üstün Z, DALDAL Y, TALAY AYDOĞAN A, ÇUBUK DEMİRALAY E (2017). An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by Using Reversed Phase Liquid Chromatography Method. Karaelmas Fen ve Mühendislik Dergisi, 7(2), 602 - 607. 10.7212/zkufbd.v7i2.589 |
| Chicago | Üstün Zehra,DALDAL Y. Doğan,TALAY AYDOĞAN Aybike,ÇUBUK DEMİRALAY Ebru An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by Using Reversed Phase Liquid Chromatography Method. Karaelmas Fen ve Mühendislik Dergisi 7, no.2 (2017): 602 - 607. 10.7212/zkufbd.v7i2.589 |
| MLA | Üstün Zehra,DALDAL Y. Doğan,TALAY AYDOĞAN Aybike,ÇUBUK DEMİRALAY Ebru An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by Using Reversed Phase Liquid Chromatography Method. Karaelmas Fen ve Mühendislik Dergisi, vol.7, no.2, 2017, ss.602 - 607. 10.7212/zkufbd.v7i2.589 |
| AMA | Üstün Z,DALDAL Y,TALAY AYDOĞAN A,ÇUBUK DEMİRALAY E An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by Using Reversed Phase Liquid Chromatography Method. Karaelmas Fen ve Mühendislik Dergisi. 2017; 7(2): 602 - 607. 10.7212/zkufbd.v7i2.589 |
| Vancouver | Üstün Z,DALDAL Y,TALAY AYDOĞAN A,ÇUBUK DEMİRALAY E An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by Using Reversed Phase Liquid Chromatography Method. Karaelmas Fen ve Mühendislik Dergisi. 2017; 7(2): 602 - 607. 10.7212/zkufbd.v7i2.589 |
| IEEE | Üstün Z,DALDAL Y,TALAY AYDOĞAN A,ÇUBUK DEMİRALAY E "An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by Using Reversed Phase Liquid Chromatography Method." Karaelmas Fen ve Mühendislik Dergisi, 7, ss.602 - 607, 2017. 10.7212/zkufbd.v7i2.589 |
| ISNAD | Üstün, Zehra vd. "An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by Using Reversed Phase Liquid Chromatography Method". Karaelmas Fen ve Mühendislik Dergisi 7/2 (2017), 602-607. https://doi.org/10.7212/zkufbd.v7i2.589 |
