TY  - JOUR
TI  - Protective Effect of Naringin on Methotrexate-Induced Testicular Apoptosis and Autophagy Via Reducing Oxidative Stress in Male Rats
AB  - In this study the possible protective effect of naringin (NRG) on methotrexate (MTX)–induceddamage on testes of rats was investigated. A total of 35 male rats were used as materials.Animals were divided into 5 groups, 7 rats in each; Group 1 (Control): A single dose injection ofphysiological saline was administered intraperitoneally on the first day. Group 2 (NRG 100): NRG(100 mg/kg b.w./ day) was given orally for 7 days. Group 3 (MTX): Single dose of 20 mg/kg of MTXwas given intraperitoneally on the first day. Group 4 (NRG 50+MTX) 50 mg/kg/day NRG was givenfor 7 days after a single intraperitoneal injection of 20 mg/kg MTX. Group 5 (NRG 100+MTX): 100mg/kg/day NRG was given for 7 days after a single intraperitoneal injection of 20 mg/kg MTX.As a results, MTX increased to malondialdehyde (MDA) levels and decreased glutathione (GSH)level and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)activities when compared to control group. On the other hand, NRG (100 mg/kg) treatment causeddecreasing the MDA levels and increa the GSH levels and SOD, CAT, and GSH-Px activities whencompared to MTX alone group. NRG 50 mg/kg treatment did not increase SOD activity.Also, MTX treatment increased caspase-3 and B-cell lymphoma 3 protein (BCL-3) expressionlevels in testicular tissue when compared to control group. However, NRG (both at the dose of 50mg/kg and 100 mg/kg) treatment lightened significantly the testis caspase-3 and BCL-3 expressionlevels when compared to MTX alone group.In conclusion, NRG (especially at the dose of 100 mg/kg) treatment decreased MTX-inducedtesticular toxicity in male rats.
AU  - KÜÇÜKLER, Sefa
AU  - KANDEMİR, Fatih Mehmet
AU  - AKSU, Emrah Hicazi
PY  - 2018
JO  - Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi
VL  - 32
IS  - 3
SN  - 1308-9323
SP  - 179
EP  - 183
DB  - TRDizin
UR  - http://search/yayin/detay/299181
ER  -