TY  - JOUR
TI  - Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae)
AB  - In this paper, genotoxic effects of the pure azadirachtin (AZA) on the larval hemocytes of model insect andstorage pest Galleria mellonella L. (Lepidoptera: Pyralidae) was investigated. The comet assay was performed to measureand analyze DNA damage in larval hemocytes at Anadolu University (Eskisehir Technical University) between 2017and 2018 years. For this purpose, sublethal AZA doses (0.5, 1, 1.5, and 2 μg/larva) given to G. mellonella larvae via insectforce feeding method were used to monitor tail intensity, tail moment and tail migration the commonly known cometparameters. These DNA damage indicators were analyzed in hemocytes obtained from larvae at 24 and 72 h post forcefeeding. All comet parameters at all doses of AZA increased in comparison with negative and positive control at 24 and72 h. At 72 h post force feeding with median lethal dose of AZA, a significant increase in DNA damage indicators wasobserved in larval hemocytes as compared with untreated groups. Consequently, this study showed that AZA causedsignificant damage in the genome of G. mellonella larvae even at sublethal doses and the comet assay was useful in themonitoring of in vivo genotoxicity of AZA in larva hemocytes.
AU  - ALTUNTAS, HULYA
AU  - DUMAN ERBAŞ, EMİNE
PY  - 2018
JO  - Biological Diversity and Conservation
VL  - 11
IS  - 3
SN  - 1308-5301
SP  - 24
EP  - 30
DB  - TRDizin
UR  - http://search/yayin/detay/299820
ER  -