Yıl: 2018 Cilt: 28 Sayı: 4 Sayfa Aralığı: 571 - 581 Metin Dili: İngilizce DOI: 10.5137/1019-5149.JTN.21789-17.1

Assessment of the MRI and Behavioral Test Results in a Focal Cerebral Ischemia-Reperfusion Model in the Rat after Separate and Combined Use of Mouse-Derived Neural Progenitor Cells, Human-Derived Neural Progenitor Cells and Atorvastatin

Öz:
AIM: To assess the efficacy of Neural progenitor cell (NPC) transplantation in ischemic stroke, and to investigate whether atorvastatinenhances therapeutic potency of NPC after stroke.MATERIAL and METHODS: The focal cerebral ischemia-reperfusion model was performed by transient occlusion of middlecerebral artery. Rats were assigned randomly to receive intracerebral transplantation of mouse NPC alone (mNPC), human NPCalone (hNPC), mouse NPC plus oral atorvastatin (mNPC+A), human NPC plus oral atorvastatin (hNPC+A), oral atorvastatin alone, orintracerebral Dulbecco’s Modified Eagle’s medium injection (control group). Adhesive removal, rotarod, cylinder tests, and magneticresonance imaging (MRI) were used for assessment of rats during 4 weeks. After sacrification on 28th day, rats were investigated byimmunofluorescent staining.RESULTS: The hNPC and mNPC groups showed significantly improved functional outcome and reduced infarct area ratio comparedwith the control group. The hNPC group had significantly better performance and lower infarct area ratio than the mNPC group.Addition of atorvastatin to stem cell therapy significantly improved functional outcome, although it did not affect the infarct area ratioon MRI. Anti-inflammatory response in the infarct area was higher in the mNPC group. NPC transplantation significantly reducedthe amount of microglia and a significant increase in the amount of astrocytes. CD8a+ T lymphocyte and granzyme B activities werenot detected in any of the subjects.CONCLUSION: Both hNPC and mNPC treatments significantly improved functional outcome, and reduced infarct area ratio afterstroke. Atorvastatin enhanced the therapeutic potency of NPCs, including neurological improvement.
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APA TAHTA A, İZGİ N, BAGCI-ONDER T, ERDAĞ E, ARAS Y, GENC C (2018). Assessment of the MRI and Behavioral Test Results in a Focal Cerebral Ischemia-Reperfusion Model in the Rat after Separate and Combined Use of Mouse-Derived Neural Progenitor Cells, Human-Derived Neural Progenitor Cells and Atorvastatin. Turkish Neurosurgery, 28(4), 571 - 581. 10.5137/1019-5149.JTN.21789-17.1
Chicago TAHTA Alican,İZGİ Nail,BAGCI-ONDER Tugba,ERDAĞ ECE,ARAS Yavuz,GENC Cetin Assessment of the MRI and Behavioral Test Results in a Focal Cerebral Ischemia-Reperfusion Model in the Rat after Separate and Combined Use of Mouse-Derived Neural Progenitor Cells, Human-Derived Neural Progenitor Cells and Atorvastatin. Turkish Neurosurgery 28, no.4 (2018): 571 - 581. 10.5137/1019-5149.JTN.21789-17.1
MLA TAHTA Alican,İZGİ Nail,BAGCI-ONDER Tugba,ERDAĞ ECE,ARAS Yavuz,GENC Cetin Assessment of the MRI and Behavioral Test Results in a Focal Cerebral Ischemia-Reperfusion Model in the Rat after Separate and Combined Use of Mouse-Derived Neural Progenitor Cells, Human-Derived Neural Progenitor Cells and Atorvastatin. Turkish Neurosurgery, vol.28, no.4, 2018, ss.571 - 581. 10.5137/1019-5149.JTN.21789-17.1
AMA TAHTA A,İZGİ N,BAGCI-ONDER T,ERDAĞ E,ARAS Y,GENC C Assessment of the MRI and Behavioral Test Results in a Focal Cerebral Ischemia-Reperfusion Model in the Rat after Separate and Combined Use of Mouse-Derived Neural Progenitor Cells, Human-Derived Neural Progenitor Cells and Atorvastatin. Turkish Neurosurgery. 2018; 28(4): 571 - 581. 10.5137/1019-5149.JTN.21789-17.1
Vancouver TAHTA A,İZGİ N,BAGCI-ONDER T,ERDAĞ E,ARAS Y,GENC C Assessment of the MRI and Behavioral Test Results in a Focal Cerebral Ischemia-Reperfusion Model in the Rat after Separate and Combined Use of Mouse-Derived Neural Progenitor Cells, Human-Derived Neural Progenitor Cells and Atorvastatin. Turkish Neurosurgery. 2018; 28(4): 571 - 581. 10.5137/1019-5149.JTN.21789-17.1
IEEE TAHTA A,İZGİ N,BAGCI-ONDER T,ERDAĞ E,ARAS Y,GENC C "Assessment of the MRI and Behavioral Test Results in a Focal Cerebral Ischemia-Reperfusion Model in the Rat after Separate and Combined Use of Mouse-Derived Neural Progenitor Cells, Human-Derived Neural Progenitor Cells and Atorvastatin." Turkish Neurosurgery, 28, ss.571 - 581, 2018. 10.5137/1019-5149.JTN.21789-17.1