The objective of the present study was to investigate theantimicrobial and anti-proliferative activities of n-hexane,chloroform, methanol and aqueous methanol extracts obtainedfrom Tanacetum argenteum subsp. argenteum aerial parts. Anti-proliferative activity was tested in vitro against four humancancer cell lines (A549: lung adenocarcinoma, Hela; cervixadenocarcinoma, HT-29: colon adenocarcinoma, MCF-7; breastadenocarcinoma) using MTT assay. Antimicrobial activity wasassessed by micro-broth dilution technique against Staphylococcusaureus ATCC 29213, Staphylococcus epidermidis ATCC 12228,Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212,Klebsiella pneumoniae ATCC 4352, Pseudomonas aeruginosaATCC 27853, Proteus mirabilis ATCC 14153, and Candidaalbicans ATCC 10231. Hexane extract inhibited proliferation of HT-29 and MCF-7 by 75 and 74% while chloroform extractagainst the same cancer cell lines displayed inhibition of 89and 73% at the concentration of 30 μg/mL, respectively. Also,chloroform extract at the same concentration showed significantanti-proliferative activity against A-549 and HeLa with inhibitionvalues of 75% and 62%. Chloroform extract exhibited moderateantibacterial activity against Staphylococcus aureus and S.epidermidis with the MIC values of 625 μg/mL. Methanol andaqueous methanol extracts showed weak antimicrobial activityagainst Staphylococcus epidermidis and Candida albicans withMIC values of 1250 μg/mL. The results showed that n-hexane andchloroform extracts have significant anticancer activity againstcancer cell lines used in this study.