Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakır watermelon (Citrullus lanatus cv. "Sürme")

ONAY, Ahmet; BAŞARAN, Davut; ADIYAMAN, Filiz; YILDIRIM, Hakan; PİRİNÇ, Vedat; IŞIKALAN, ÇİĞDEM

Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakır watermelon (Citrullus lanatus cv. "Sürme")

Vedat PİRİNÇ, (Dicle Üniversitesi, Ziraat Fakültesi, Diyarbakır, Türkiye)

Ahmet ONAY, (Girilmedi)

Hakan YILDIRIM, (Girilmedi)

Filiz ADIYAMAN, (Girilmedi)

Çiğdem IŞIKALAN, (Girilmedi)

Davut BAŞARAN (Girilmedi)

Turkish Journal of Biology

1 0

Yıl: 2003 Cilt: 27 Sayı: 2 Sayfa Aralığı: 101 - 105 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakır watermelon (Citrullus lanatus cv. "Sürme")

Öz:

Diploid Diyarbakır karpuzunun (CitruIIus lanatus ev. "Sürme") kotiledonlarından 3 haftalık kültür sonucunda adventif sürgünler oluşturuldu. Sürgün organojenezisi üzerine iki tip sitokininin; benzil aminopürin (BA) ve kinetin (Kin) farklı konsantrasyonlarının etkileri araştırıldı. Eksplant başına oluşan sürgün sayısı bakımından en iyi sonuç, 0.5 mg/1 BA içeren besi ortamından elde edilirken bu oran aynı zamanda kinetinin en iyi sonuç veren konsantrasyonundan (1 mg/l) yaklaşık %50 daha fazla olduğu tespit edilmiştir. Elde edilen sürgünlerin (1-2 cm) in vitro ortamda köklenmesi; Naftalen Asetik Asit'le (NAA) desteklenen MS besi ortamına aktarılmasıyla elde edilmiştir. Rejenere edilen bitkilerin %50'den fazlası başarılı bir şekilde toprağa aktarılmıştır. Sonuç olarak, yüksek kaliteli diploid Diyarbakır karpuzunun in vitro yöntemle çoğaltılabileceği ve geliştirilen bu yöntemin, karpuz çeşitlerinin ıslahında kullanılabileceği kanısındayız.

Anahtar Kelime: filiz eksplant karpuz doku kültürü sitokininler benziladenin kinetin fideler Citrullus lanatus organ oluşumu

Konular: Biyoloji

Diploid Diyarbakır karpuzu (Citrullus lanatus cv. "Sürme") kotiledonlarından adventif sürgün organogenezisi ve bitki rejenerasyonu

Öz:

Adventitious shoots were obtained from the diploid Diyarbakır watermelon cultivar "Sürme" by culturing cotyledons on shoot regeneration medium for 3 weeks. The effects of two cytokinins, benzyl amino purine (BA) and kinetine (Kin), on shoot organogenesis were examined. The number of shoots per expiant were approximately more than 50% higher when 0.5 mg/1 BA was used compared to the most effective kinetin concentration (1 mg/1). Plants were obtained by transferring 1 to 2 cm shoots to MS medium supplemented with 1 -4 mg/1 naphthalene acetic acid. Most of the regenerated plants (more than 50%) were successfully transplanted to the field. This demonstrates that in vitro propagation can be used to produce high quality diploid Diyarbakır watermelon for use in breeding lines.

Anahtar Kelime: benzyladenine kinetin seedlings Citrullus lanatus organogenesis shoots explants watermelons tissue culture cytokinins

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	PİRİNÇ V, ONAY A, YILDIRIM H, ADIYAMAN F, IŞIKALAN Ç, BAŞARAN D (2003). Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakır watermelon (Citrullus lanatus cv. "Sürme"). , 101 - 105.
Chicago	PİRİNÇ Vedat,ONAY Ahmet,YILDIRIM Hakan,ADIYAMAN Filiz,IŞIKALAN ÇİĞDEM,BAŞARAN Davut Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakır watermelon (Citrullus lanatus cv. "Sürme"). (2003): 101 - 105.
MLA	PİRİNÇ Vedat,ONAY Ahmet,YILDIRIM Hakan,ADIYAMAN Filiz,IŞIKALAN ÇİĞDEM,BAŞARAN Davut Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakır watermelon (Citrullus lanatus cv. "Sürme"). , 2003, ss.101 - 105.
AMA	PİRİNÇ V,ONAY A,YILDIRIM H,ADIYAMAN F,IŞIKALAN Ç,BAŞARAN D Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakır watermelon (Citrullus lanatus cv. "Sürme"). . 2003; 101 - 105.
Vancouver	PİRİNÇ V,ONAY A,YILDIRIM H,ADIYAMAN F,IŞIKALAN Ç,BAŞARAN D Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakır watermelon (Citrullus lanatus cv. "Sürme"). . 2003; 101 - 105.
IEEE	PİRİNÇ V,ONAY A,YILDIRIM H,ADIYAMAN F,IŞIKALAN Ç,BAŞARAN D "Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakır watermelon (Citrullus lanatus cv. "Sürme")." , ss.101 - 105, 2003.
ISNAD	PİRİNÇ, Vedat vd. "Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakır watermelon (Citrullus lanatus cv. "Sürme")". (2003), 101-105.

APA	PİRİNÇ V, ONAY A, YILDIRIM H, ADIYAMAN F, IŞIKALAN Ç, BAŞARAN D (2003). Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakır watermelon (Citrullus lanatus cv. "Sürme"). Turkish Journal of Biology, 27(2), 101 - 105.
Chicago	PİRİNÇ Vedat,ONAY Ahmet,YILDIRIM Hakan,ADIYAMAN Filiz,IŞIKALAN ÇİĞDEM,BAŞARAN Davut Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakır watermelon (Citrullus lanatus cv. "Sürme"). Turkish Journal of Biology 27, no.2 (2003): 101 - 105.
MLA	PİRİNÇ Vedat,ONAY Ahmet,YILDIRIM Hakan,ADIYAMAN Filiz,IŞIKALAN ÇİĞDEM,BAŞARAN Davut Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakır watermelon (Citrullus lanatus cv. "Sürme"). Turkish Journal of Biology, vol.27, no.2, 2003, ss.101 - 105.
AMA	PİRİNÇ V,ONAY A,YILDIRIM H,ADIYAMAN F,IŞIKALAN Ç,BAŞARAN D Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakır watermelon (Citrullus lanatus cv. "Sürme"). Turkish Journal of Biology. 2003; 27(2): 101 - 105.
Vancouver	PİRİNÇ V,ONAY A,YILDIRIM H,ADIYAMAN F,IŞIKALAN Ç,BAŞARAN D Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakır watermelon (Citrullus lanatus cv. "Sürme"). Turkish Journal of Biology. 2003; 27(2): 101 - 105.
IEEE	PİRİNÇ V,ONAY A,YILDIRIM H,ADIYAMAN F,IŞIKALAN Ç,BAŞARAN D "Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakır watermelon (Citrullus lanatus cv. "Sürme")." Turkish Journal of Biology, 27, ss.101 - 105, 2003.
ISNAD	PİRİNÇ, Vedat vd. "Adventitious shoot organogenesis and plant regeneration from cotyledons of diploid Diyarbakır watermelon (Citrullus lanatus cv. "Sürme")". Turkish Journal of Biology 27/2 (2003), 101-105.