Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological features

KIVANÇ, Merih; KÜÇÜK, Çiğdem

Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological features

Çiğdem KÜÇÜK, (Anadolu Üniversitesi, Eskişehir, Türkiye)

Merih KIVANÇ (Anadolu Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Eskişehir, Türkiye)

Turkish Journal of Biology

1 0

Yıl: 2003 Cilt: 27 Sayı: 4 Sayfa Aralığı: 247 - 253 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological features

Öz:

Trichoderma spp.'nin biyolojik kontrol ajani olarak kullanildigi genellikle bilinmektedir. Bu çalismada, 31 degisik toprak örneginden Trichoderma izolati izole edilmistir. Toprak örnekleri Eskisehir ve çevresinden alinmistir. Bu izolatlarin farkli bitki patojeni funguslara karsi antifungal, biyokimyasal ve fizyolojik etkileri arastirilmistir. Trichoderma harzianum T9, T10, T15 ve T19'un filtratlari bitki patojenlerinden Fusarium culmarum, F. oxysporum, F. moniliforme, Rhizoctonia solani, Sclerotium rolfsii, Gaeumannomyces graminis var. tritici ve Drechslera sorokiniana'a etkili olmustur. T. harzianum T19, birden fazla bitki patojenini inhibe etmistir. F. oxysporum, filtrat deneylerinde en dirençli patojen olarak belirlenmistir. Tüm izolatlar, jelatin hidrolizi, inhibitör maddelerde, farkli pH ve sicaklik düzeylerinde gelismeleri gibi fizyolojik testlerde farkli özellikler göstermislerdir.

Anahtar Kelime: SDS-PAGE antifungal özellikler kitinaz biyolojik kontrol ajanları mantar Trichoderma harzianum engelleme fiziksel özellikler kitin enzim aktivitesi bitki patojenleri

Konular: Biyoloji

Trichoderma Spp.' nın izolasyonu ve antifungal, biyokimyasal, fizyolojik özelliklerinin tespiti

Öz:

It is well known that Trichoderma spp. can be used as a biological control agent. In this study, Trichoderma isolates were obtained from 31 different Eskisehir soil samples. The biocontrol and antifungal effects of these isolates against various plant pathogen fungi were determined. We found that all filtrates of Trichoderma harzianumT9, T10, T15 and T19 were effective against plant pathogens Fusarium culmarum, F. oxysporum, F. moniliforme, Rhizoctonia solani, Sclerotium rolfsii, Gaeumannomyces graminis var. tritici and Drechslera sorokiniana. Among these isolates, T. harzianum T19 showed a wide range of inhibitory effects on plant pathogens. F. oxysporum was found to be the most resistant to the filtrates of the strains above. All isolates showed different behaviors depending on the physiological tests carried out such as growth in the presence inhibitory substrates, pH limits of growth and hydrolysis of gelatin. T. harzianum isolates were grown on the chitin, which is the sole carbon source. The chitinase activity determined from T. harzianumT15 by SDS-PAGE was nearly 73 kDa.

Anahtar Kelime: inhibition physical properties chitin enzyme activity plant pathogens SDS-PAGE antifungal properties chitinase biological control agents fungi Trichoderma harzianum

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KÜÇÜK Ç, KIVANÇ M (2003). Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological features. , 247 - 253.
Chicago	KÜÇÜK Çiğdem,KIVANÇ Merih Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological features. (2003): 247 - 253.
MLA	KÜÇÜK Çiğdem,KIVANÇ Merih Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological features. , 2003, ss.247 - 253.
AMA	KÜÇÜK Ç,KIVANÇ M Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological features. . 2003; 247 - 253.
Vancouver	KÜÇÜK Ç,KIVANÇ M Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological features. . 2003; 247 - 253.
IEEE	KÜÇÜK Ç,KIVANÇ M "Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological features." , ss.247 - 253, 2003.
ISNAD	KÜÇÜK, Çiğdem - KIVANÇ, Merih. "Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological features". (2003), 247-253.

APA	KÜÇÜK Ç, KIVANÇ M (2003). Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological features. Turkish Journal of Biology, 27(4), 247 - 253.
Chicago	KÜÇÜK Çiğdem,KIVANÇ Merih Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological features. Turkish Journal of Biology 27, no.4 (2003): 247 - 253.
MLA	KÜÇÜK Çiğdem,KIVANÇ Merih Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological features. Turkish Journal of Biology, vol.27, no.4, 2003, ss.247 - 253.
AMA	KÜÇÜK Ç,KIVANÇ M Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological features. Turkish Journal of Biology. 2003; 27(4): 247 - 253.
Vancouver	KÜÇÜK Ç,KIVANÇ M Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological features. Turkish Journal of Biology. 2003; 27(4): 247 - 253.
IEEE	KÜÇÜK Ç,KIVANÇ M "Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological features." Turkish Journal of Biology, 27, ss.247 - 253, 2003.
ISNAD	KÜÇÜK, Çiğdem - KIVANÇ, Merih. "Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological features". Turkish Journal of Biology 27/4 (2003), 247-253.