Adipokine Levels and Perilipin Gene Polymorphisms in Obese Turkish Adolescents with Non-Alcoholic Fatty Liver Disease

ERDUR, Cahit Barış; Tokgöz, Yavuz; arslan, nur; ERDAL BAĞRIYANIK, ŞERİFE ESRA; KÜME, TUNCAY; TERLEMEZ, Semiha; sayin, oya; AKBARİ, Soheil

doi:10.5152/etd.2018.0010

Adipokine Levels and Perilipin Gene Polymorphisms in Obese Turkish Adolescents with Non-Alcoholic Fatty Liver Disease

Yavuz TOKGÖZ, (Department of Pediatric Gastroenterology, Hepatology and Nutrition, Adnan Menderes University Faculty of Medicine)

Cahit Barış ERDUR, (Department of Pediatric Gastroenterology, Hepatology and Nutrition, Dokuz Eylül University Faculty of Medicine)

Soheil AKBARİ, (Department of Medical Biology, Dokuz Eylül University Faculty of Medicine)

Tuncay KÜME, (Department of Biochemistry, Dokuz Eylül University Faculty of Medicine)

Oya SAYIN, (Research Laboratory, Dokuz Eylül University Faculty of Medicine)

Semiha TERLEMEZ, (Department of Pediatrics, Adnan Menderes University Faculty of Medicine)

ŞERİFE ESRA ERDAL BAĞRIYANIK, (Department of Medical Biology, Dokuz Eylül University Faculty of Medicine)

Nur ARSLAN (Department of Pediatric Gastroenterology, Hepatology and Nutrition, Dokuz Eylül University Faculty of Medicine)

Erciyes Medical Journal

2 0

Yıl: 2018 Cilt: 40 Sayı: 2 Sayfa Aralığı: 65 - 69 Metin Dili: İngilizce DOI: 10.5152/etd.2018.0010 İndeks Tarihi: 23-08-2019

Adipokine Levels and Perilipin Gene Polymorphisms in Obese Turkish Adolescents with Non-Alcoholic Fatty Liver Disease

Öz:

Objective: The aim of the present study was to evaluate the relationship between adipokines and perilipin (PLIN) polymorphisms with non-alcoholic fatty liver disease (NAFLD). Methods: Obese Turkish adolescents were assessed in the study. The patients were divided into two groups: obese (NAFLD and non-NAFLD) and non-obese. Serum leptin, adiponectin, resistin, and ghrelin levels and PLIN gene analysis (PLIN 1, 4, and 6) were studied in all patients and healthy control group. Data obtained were compared with those of healthy control group. Results: Overall, 83 obese adolescents with NAFLD, 123 obese adolescents with normal liver, and 102 healthy non-obese adolescents as the control group were evaluated. No significant difference was observed in terms of serum adipokine (leptin, adiponectin, resistin, and ghrelin) levels in patients with NAFLD and non-NAFLD obese adolescents. The incidence of major alleles of PLIN 6 genotype in obese adolescents without NAFLD was slightly higher than that in the control group (p=0.06). PLIN 6 minor allele incidence was significantly lower (p=0.01), and PLIN 4 major allele frequency in patients with NAFLD was slightly lower than those in the control group (p=0.05). Conclusion: These findings suggested that no adipokine role was determined in the development of fatty liver in obese adolescents. The low rate in PLIN 6 minor allele can be a risk factor for NAFLD in adolescents.

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

Birkenfeld AL, Shulman GI. Nonalcoholic fatty liver disease, hepatic insulin resistance, and type 2 diabetes. Hepatology 2014;59(2):71323. [CrossRef]
Arslan N. Obesity, fatty liver disease and intestinal microbiota.World J Gastroenterol 2014;28;20(44): 16452-63.
Paschos P, Paletas K. Non alcoholic fatty liver disease and metabolicsyndrome. Hippokratia 2009; 13(1): 9–19.
Osumi T, Kuramoto K. Heart lipid droplets and lipid droplet-binding proteins: Biochemistry, physiology, and pathology. Exp Cell Res 2016; 340(2): 198-204. [CrossRef]
Brasaemle DL. The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysis. J Lipid Res 2007; 48: 2547-59. [CrossRef]
Brasaemle DL, Subramanian V, Garcia A, Marcinkiewicz A, Rothenberg A. Perilipin A and the control of triacylglycerol metabolism.Mol Cell Biochem 2009; 326: 15-21. [CrossRef]
Imai Y, Boyle S, Varela GM, Caron E, Yin X, Dhir R, et al. Effects of perilipin 2 antisense oligonucleotide treatment on hepatic lipid metabolism and gene expression. Physiol Genomics 2012; 44: 1125-1131. [CrossRef]
Carr RM, Patel RT, Rao V, Dhir R, Graham MJ, Crooke RM, et al. Reduction of TIP47 improves hepatic steatosis and glucose homeostasis in mice. Am J Physiol Regul Integr Comp Physiol 2012; 302: 996-1003. [CrossRef]
Ikura Y, Caldwell SH. Lipid droplet-associated proteins in alcoholic liver disease: a potential linkage with hepatocellular damage. Int J Clin Exp Pathol 2015;8(8): 8699-708
Caldwell SH, Ikura Y, Iezzoni JC, Liu Z. Has natural selection in human populations produced two types of metabolic syndrome (with and without fatty liver)? J Gastroenterol Hepatol 2007;22 Suppl 1: 11-19. [CrossRef]
Straub BK, Stoeffel P, Heid H, Zimbelmann R, Schirmacher P. Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 2008; 47: 193646. [CrossRef]
Smith CE, Ordovás JM. Update on perilipin polymorphisms and obesity. Nutr Rev 2012; 70(10): 611–621. [CrossRef]
Tokgöz Y, Işık IA, Akbari S, Kume T, Sayın O, Erdal E et al. Perilipin polymorphisms are risk factors for the development of obesity in adolescents? A case-control study.Lipids Health Dis 2017; 9: 16(1): 52.
Cole TJ, Lobstein T.Extended international (IOTF) body mass index cut-offs for thinness, overweight and obesity. Pediatr Obes 2012; 7: 284-94. [CrossRef]
Quin SF, Gosink BB. Characteristic sonographic signs of hepatic fatty infiltration. Am J Roentgenol 1985; 145: 753-5. [CrossRef]
Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972; 18: 499-502.
Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC. Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 1985; 28: 412-9. [CrossRef]
Keskin M, Kurtoglu S, Kendirci M, Atabek ME, Yazici C. Homeostasis model assessment is more reliable than the fasting glucose/insulin ratio and quantitative insulin sensitivity check index for assessing insulin resistance among obese children and adolescents.Pediatrics 2005;115(4):e500-3. [CrossRef]
1Birkenfeld AL, Shulman GI. Non Alcoholic Fatty Liver Disease, Hepatic Insulin Resistance and Type 2 Diabetes. Hepatology 2014; 59(2): 713–23. [CrossRef]
Temple JL, Cordero P, Li J, Nguyen V, Oben JA. A Guide to NonAlcoholic Fatty Liver Disease in Childhood and Adolescence. Int J Mol Sci 2016; 17: 1-36. [CrossRef]
Gastaldelli A, Harrison S, Belfort-Aguiar R,Hardies J, Balas B, Schenker S, et al. Pioglitazone in the treatment of NASH: The role of adiponectin. Aliment Pharmacol Ther 2010; 32: 769–75. [CrossRef]
Petta S, Gastaldelli A, Rebelos E, Bugianesi E, Messa P, Miele L, et al. Pathophysiology of Non Alcoholic Fatty Liver Disease.Int J Mol Sci 2016; 11: 17(12).
. Polyzos SA, Aronis KN, Kountouras J, Raptis DD, Vasiloglou MF, Mantzoros CS. Circulating leptin in non-alcoholic fatty liver disease: A systematic review and meta-analysis. Diabetologia 2016; 59: 30-43. [CrossRef]
Stojsavljević S, Gomerčić Palčić M, Virović Jukić L, Smirčić Duvnjak L, Duvnjak M. Adipokines and proinflammatory cytokines, the key mediators in the pathogenesis of nonalcoholic fatty liver disease. World J Gastroenterol 2014; 20: 18070-91. [CrossRef]
. Sztalryd C, Kimmel AR. Perilipins: Lipid Droplet Coat Proteins Adapted for Tissue-Specific Energy Storage and Utilization, and Lipid Cytoprotection. Biochimie 2014; 96: 96-101. [CrossRef]
Yoshihiro Ikura, Stephen H Caldwell. Lipid droplet-associated proteins in alcoholic liver disease: a potential linkage with hepatocellular damageInt J Clin Exp Pathol 2015; 8(8): 8699-708.
Carr RM, Ahima RS. Pathophysiology of lipid droplet proteins in liver diseases. Exp Cell Res 2016;15;340(2):187-92.
. Qi L, Shen H, Larson I, Schaefer EJ, Greenberg AS, Tregouet DA, et al. Gender-specific association of a perilipin gene haplotype with obesity risk in a white population. Obes Res 2004; 12: 1758–65. [CrossRef]
Mottagui-Tabar S, Ryden M, Lofgren P, Faulds G, Hoffstedt J, Brookes AJ, et al. Evidence for an important role of perilipin in the regulation of human adipocyte lipolysis. Diabetologia 2003; 46: 789-97. [CrossRef]
. Perez-Martinez P, Yiannakouris N, Lopez-Miranda J, Arnett D, Tsai M, Galan E, et al. Postprandial triacylglycerol metabolism is modified by the presence of genetic variation at the perilipin (PLIN) locus in 2 white populations. Am J Clin Nutr 2008; 87 :744–52. [CrossRef]
Sone Y, Yamaguchi K, Fujiwara A, Kido T, Kawahara K, Ishiwaki A, et al. Association of lifestyle factors, polymorphisms in adiponectin, perilipin and hormone senditive lipase, and clinical markers in Japanese males. J Nutr Sci Vitaminol 2010; 56: 123-31. [CrossRef]
Deram S, Nicolau CY, Perez-Martinez P, Guazzelli I, Halpern A, Wajchenberg BL, et al. Effects of perilipin (PLIN) gene variation on metabolic syndrome risk and weight loss in obese children and adolescents. J Clin Endocrinol Metab 2008; 93: 4933-40. [CrossRef]
Meirhaeghe A, Thomas S, Ancot F, Cottel D, Arveiler D, Ferrières J, et al. Study of the impact of perilipin polymorphisms in a French population.J Negat Results Biomed 2006; 12: 5-10. [CrossRef]
Qi L, Tai ES, Tan CE, Shen H, Chew SK, Greenberg AS, et al. Intragenic linkage disequilibrium structure of the human perilipin gene (PLIN) and haplotype association with increased obesity risk in a multiethnic Asian population. J Mol Med 2005; 83: 448-56. [CrossRef]
Soenen S, Mariman EC, Vogels N, Bouwman FG, den Hoed M, Brown L, et al. Relationship between perilipin gene polymorphisms and body weight and body composition during weight loss and weight maintenance. Physiol Behav 2009; 96: 723-8.[CrossRef]

APA	Tokgöz Y, ERDUR C, AKBARİ S, KÜME T, sayin o, TERLEMEZ S, ERDAL BAĞRIYANIK Ş, arslan n (2018). Adipokine Levels and Perilipin Gene Polymorphisms in Obese Turkish Adolescents with Non-Alcoholic Fatty Liver Disease. , 65 - 69. 10.5152/etd.2018.0010
Chicago	Tokgöz Yavuz,ERDUR Cahit Barış,AKBARİ Soheil,KÜME TUNCAY,sayin oya,TERLEMEZ Semiha,ERDAL BAĞRIYANIK ŞERİFE ESRA,arslan nur Adipokine Levels and Perilipin Gene Polymorphisms in Obese Turkish Adolescents with Non-Alcoholic Fatty Liver Disease. (2018): 65 - 69. 10.5152/etd.2018.0010
MLA	Tokgöz Yavuz,ERDUR Cahit Barış,AKBARİ Soheil,KÜME TUNCAY,sayin oya,TERLEMEZ Semiha,ERDAL BAĞRIYANIK ŞERİFE ESRA,arslan nur Adipokine Levels and Perilipin Gene Polymorphisms in Obese Turkish Adolescents with Non-Alcoholic Fatty Liver Disease. , 2018, ss.65 - 69. 10.5152/etd.2018.0010
AMA	Tokgöz Y,ERDUR C,AKBARİ S,KÜME T,sayin o,TERLEMEZ S,ERDAL BAĞRIYANIK Ş,arslan n Adipokine Levels and Perilipin Gene Polymorphisms in Obese Turkish Adolescents with Non-Alcoholic Fatty Liver Disease. . 2018; 65 - 69. 10.5152/etd.2018.0010
Vancouver	Tokgöz Y,ERDUR C,AKBARİ S,KÜME T,sayin o,TERLEMEZ S,ERDAL BAĞRIYANIK Ş,arslan n Adipokine Levels and Perilipin Gene Polymorphisms in Obese Turkish Adolescents with Non-Alcoholic Fatty Liver Disease. . 2018; 65 - 69. 10.5152/etd.2018.0010
IEEE	Tokgöz Y,ERDUR C,AKBARİ S,KÜME T,sayin o,TERLEMEZ S,ERDAL BAĞRIYANIK Ş,arslan n "Adipokine Levels and Perilipin Gene Polymorphisms in Obese Turkish Adolescents with Non-Alcoholic Fatty Liver Disease." , ss.65 - 69, 2018. 10.5152/etd.2018.0010
ISNAD	Tokgöz, Yavuz vd. "Adipokine Levels and Perilipin Gene Polymorphisms in Obese Turkish Adolescents with Non-Alcoholic Fatty Liver Disease". (2018), 65-69. https://doi.org/10.5152/etd.2018.0010

APA	Tokgöz Y, ERDUR C, AKBARİ S, KÜME T, sayin o, TERLEMEZ S, ERDAL BAĞRIYANIK Ş, arslan n (2018). Adipokine Levels and Perilipin Gene Polymorphisms in Obese Turkish Adolescents with Non-Alcoholic Fatty Liver Disease. Erciyes Medical Journal, 40(2), 65 - 69. 10.5152/etd.2018.0010
Chicago	Tokgöz Yavuz,ERDUR Cahit Barış,AKBARİ Soheil,KÜME TUNCAY,sayin oya,TERLEMEZ Semiha,ERDAL BAĞRIYANIK ŞERİFE ESRA,arslan nur Adipokine Levels and Perilipin Gene Polymorphisms in Obese Turkish Adolescents with Non-Alcoholic Fatty Liver Disease. Erciyes Medical Journal 40, no.2 (2018): 65 - 69. 10.5152/etd.2018.0010
MLA	Tokgöz Yavuz,ERDUR Cahit Barış,AKBARİ Soheil,KÜME TUNCAY,sayin oya,TERLEMEZ Semiha,ERDAL BAĞRIYANIK ŞERİFE ESRA,arslan nur Adipokine Levels and Perilipin Gene Polymorphisms in Obese Turkish Adolescents with Non-Alcoholic Fatty Liver Disease. Erciyes Medical Journal, vol.40, no.2, 2018, ss.65 - 69. 10.5152/etd.2018.0010
AMA	Tokgöz Y,ERDUR C,AKBARİ S,KÜME T,sayin o,TERLEMEZ S,ERDAL BAĞRIYANIK Ş,arslan n Adipokine Levels and Perilipin Gene Polymorphisms in Obese Turkish Adolescents with Non-Alcoholic Fatty Liver Disease. Erciyes Medical Journal. 2018; 40(2): 65 - 69. 10.5152/etd.2018.0010
Vancouver	Tokgöz Y,ERDUR C,AKBARİ S,KÜME T,sayin o,TERLEMEZ S,ERDAL BAĞRIYANIK Ş,arslan n Adipokine Levels and Perilipin Gene Polymorphisms in Obese Turkish Adolescents with Non-Alcoholic Fatty Liver Disease. Erciyes Medical Journal. 2018; 40(2): 65 - 69. 10.5152/etd.2018.0010
IEEE	Tokgöz Y,ERDUR C,AKBARİ S,KÜME T,sayin o,TERLEMEZ S,ERDAL BAĞRIYANIK Ş,arslan n "Adipokine Levels and Perilipin Gene Polymorphisms in Obese Turkish Adolescents with Non-Alcoholic Fatty Liver Disease." Erciyes Medical Journal, 40, ss.65 - 69, 2018. 10.5152/etd.2018.0010
ISNAD	Tokgöz, Yavuz vd. "Adipokine Levels and Perilipin Gene Polymorphisms in Obese Turkish Adolescents with Non-Alcoholic Fatty Liver Disease". Erciyes Medical Journal 40/2 (2018), 65-69. https://doi.org/10.5152/etd.2018.0010