The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver

Kadriye DEMİRKAYA, (Department of Endodontics, Gülhane Education and Research Hospital, University of Health Sciences, Ankara, Turkey)
Birsen Can DEMİRDÖĞEN, (Department of Biomedical Engineering, TOBB University of Economics and Technology, Ankara, Turkey)
Zeynep ÖNCEL TORUN, (Balgat Oral and Dental Health Center, Ankara, Turkey)
Onur ERDEM, (Department of Toxicology, Gülhane Education and Research Hospital, University of Health Sciences, Ankara, Turkey)
Yaşar Meriç TUNCA (Department of Endodontics, Near East University, Nicosia, Cyprus)
Türk Biyokimya Dergisi
0 0
Yıl: 2018 Cilt: 43 Sayı: 3 Sayfa Aralığı: 333 - 341 Metin Dili: İngilizce DOI: 10.1515/tjb-2016-0263 İndeks Tarihi: 07-10-2019

The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver

Öz:
Objective: The aim of this study was to evaluate the effectsof hydraulic calcium silicate endodontic cements, MTAAngelus, MTA Fillapex, and Theracal LC, on erythrocyteand liver oxidative stress parameters of rats.Methods: Right upper incisor of each rat was extractedand polyethylene tubes containing the dental cements,or left empty for the control group, were inserted into theextraction socket. Blood and liver samples of each animalwere obtained after 7, 30, or 60 days. Thiobarbituric acidreactive substances (TBARS) levels and catalase (CAT),superoxide dismutase (SOD), and glutathione peroxidase(GPx) activities were determined by spectrophotometry.Results: Erythrocyte and liver TBARS levels, and CAT andSOD enzymatic activities were significantly increased indental cement applied groups compared with controls onday 7. The highest erythrocyte and liver TBARS concentrationswere observed in the MTA Angelus group on day 7of exposure. On day 30, erythrocyte CAT activity remainedmarkedly high, but the other parameters returned toalmost normal levels. On day 60, all parameters weresimilar between the control and the experimental groups.Conclusions: This is the first study to show that TBARSlevels and antioxidant enzyme activities are transientlyincreased as a result of dental cement application.
Anahtar Kelime:

Konular: Diş Hekimliği Toksikoloji Biyokimya ve Moleküler Biyoloji Farmakoloji ve Eczacılık

Hidrolik kalsiyum silikat içerikli endodontik materyallerin eritrositler ve karaciğerdeki oksidatif stres üzerindeki etkileri

Öz:
Amaç: Bu çalışmanın amacı, hidrolik kalsiyum silikat içerikli endodontik simanların (MTA Angelus, MTA Fillapex ve Theracal LC), sıçanların eritrositlerinde ve karaciğerlerindeki oksidatif stres parametreleri üzerine etkisini değerlendirmektir. Yöntem: Her sıçanın sağ üst kesici dişi çekildi; diş simanlarını içeren ya da kontrol grubu için boş bırakılan iki ucu açık polietilen tüpler diş çekim soketine yerleştirildi. Her hayvanın kan ve karaciğer örnekleri 7, 30 veya 60 gün sonra elde edildi. Tiobarbitürik asit reaktif maddeler (TBARS) seviyeleri ve katalaz (CAT), süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GPx) aktiviteleri spektrofotometri ile belirlendi. Bulgular: Eritrosit ve karaciğer TBARS seviyeleri ve CAT ve SOD enzim aktiviteleri 7. günde diş simanı uygulanmış gruplarda kontrollere kıyasla anlamlı şekilde artmıştı. En yüksek eritrosit ve karaciğer TBARS konsantrasyonları, maruziyetin 7. gününde MTA Angelus grubunda gözlendi.30. günde, eritrosit CAT aktivitesi belirgin olarak yüksek kaldı, ancak diğer parametreler neredeyse normal seviyelere döndü. 60. günde, tüm parametreler kontrol grubu ile deney grupları arasında benzerdi. Sonuç: Bu, TBARS düzeyleri ve antioksidan enzim aktivitelerinin, diş simanı uygulamasının bir sonucu olarak geçici olarak arttığını gösteren ilk çalışmadır.
Anahtar Kelime:

Konular: Diş Hekimliği Toksikoloji Biyokimya ve Moleküler Biyoloji Farmakoloji ve Eczacılık
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • 1. Kum KY, Kim EC, Yoo YJ, Zhu Q, Safavi K, Bae KS, et al. Trace metal contents of three tricalcium silicate materials: MTA Angelus, Micro Mega MTA and Bioaggregate. Int Endod J 2014;47: 704–10.
  • 2. Torabinejad M, Parirokh M. Mineral trioxide aggregate: a comprehensive literature review – part II: leakage and biocompatibility investigations. J Endod 2010;36:190–202.
  • 3. Hakki SS, Bozkurt SB, Hakki EE, Belli S. Effects of mineral trioxide aggregate on cell survival, gene expression associated with mineralized tissues, and biomineralization of cementoblasts. J Endod 2009;35:513–9.
  • 4. MTA Angelus Reparative Cement Technical Profile. Available at: http://www.angelusdental.com/img/arquivos/mta_technical_ profile_download.pdf. Accessed: July 2016.
  • 5. MTA Fillapex Endodontic Sealer Scientific Profile. Available at: http://www.angelusdental.com/img/arquivos/mta_fillapex_technical_ profile_download.pdf. Accessed: July 2016.
  • 6. Vitti RP, Prati C, Silva EJ, Sinhoreti MA, Zanchi CH, de Souza e Silva MG, et al. Physical properties of MTA Fillapex sealer. J Endod 2013;39:915–8.
  • 7. Gandolfi MG, Siboni F, Prati C. Chemical-physical properties of TheraCal, a novel light-curable MTA-like material for pulp capping. Int Endod J 2012;45:571–9.
  • 8. Poggio C, Arciola CR, Beltrami R, Monaco A, Dagna A, Lombardini M, et al. Cytocompatibility and antibacterial properties of capping materials. ScientificWorldJournal 2014;2014:181945.
  • 9. Michel R. Trace metal analysis in biocompatibility testing. Crit Rev Biocompat 1987;3:235–317.
  • 10. Geis-Gerstorfer J, Sauer KH, Pässler K. Ion release from Ni-Cr-Mo and Co-Cr-Mo casting alloys. Int J Prosthodont 1991;4:152–8.
  • 11. Wataha JC, Craig RG, Hanks CT. The release of elements of dental casting alloys into cell-culture medium. J Dent Res 1991;70:1014–8.
  • 12. Bumgardner JD, Lucas LC. Cellular response to metallic ions released from nickel-chromium dental alloys. J Dent Res 1995;74:1521–7.
  • 13. Nejatidanesh F, Savabi O, Yazdanparast A. An investigation on metallic ion release from four dental casting alloys. J Dent (Tehran) 2005;2:168–73.
  • 14. Demirkaya K, Can Demirdöğen B, Öncel Torun Z, Erdem O, Çetinkaya S, Akay C. In vivo evaluation of the effects of hydraulic calcium silicate dental cements on plasma and liver aluminium levels of rats. EurJ Oral Sci 2016;124:75–81.
  • 15. Grazziotin-Soares R, Nekoofar MH, Davies TE, Bafail A, Alhaddar E, Hübler R, et al. Effect of bismuth oxide on white mineral trioxide aggregate: chemical characterization and physical properties. Int Endod J 2014;7:520–33.
  • 16. Perlemuter G, Davit-Spraul A, Cosson C, Conti M, Bigorgne A, Paradis V, et al. Increase in liver antioxidant enzyme activities in non-alcoholic fatty liver disease. Liver Int 2005;25:946–53.
  • 17. Mahoney DJ, Kaczor JJ, Bourgeois J, Yasuda N, Tarnopolsky MA. Oxidative stress and antioxidant enzyme upregulation in SOD1- G93A mouse skeletal muscle. Muscle Nerve 2006;33:809–16.
  • 18. Cintra LT, Bernabé PF, de Moraes IG, Gomes-Filho JE, Okamoto T, Consolaro A, et al. Evaluation of subcutaneous and alveolar implantation surgical sites in the study of the biological properties of root-end filling endodontic materials. J Appl Oral Sci 2010;18:75–82.
  • 19. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animals and tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351–8.
  • 20. Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121–6. 21. Aydin A, Orhan H, Sayal A, Ozata M, Sahin G, Işimer A. Oxidative stress and nitric oxide related parameters in type II diabetes mellitus: effects of glycemic control. Clin Biochem 2001;34: 65–70.
  • 22. Demirkaya K, Demirdöğen BC, Torun ZÖ, Erdem O, Çırak E, Tunca YM. Brain aluminium accumulation and oxidative stress in the presence of calcium silicate dental cements. Hum Exp Toxicol 2016. DOI: 10.1177/0960327116679713.
  • 23. Wu Z, Du Y, Xue H, Wu Y, Zhou B. Aluminum induces neurodegeneration and its toxicity arises from increased iron accumulation and reactive oxygen species (ROS) production. Neurobiol Aging 2012;33:199.e1–199.e12.
  • 24. Degrood ME, Oguntebi BR, Cunningham CJ, Pink R. A comparison of tissue reactions to Ketac-Fil and Amalgam. J Endod 1995;21:65–9.
  • 25. Nary Filho H, Okamoto T. Evaluation of the hapset implants (Hydroxyapatite/calcium sulphate) biocompatibility in dental extraction wounds. Histological study in rats. J Appl Oral Sci 1996;4:55–64.
  • 26. Gomes-Filho JE, de Moraes Costa MM, Cintra LT, Duarte PC, Takamiya AS, Lodi CS, et al. Evaluation of rat alveolar bone response to Angelus MTA or experimental light-cured mineral trioxide aggregate using fluorochromes. J Endod 2011;37:250–4.
  • 27. American Dental Association. Council on dental material and devices. Recomended standard practices for biological evaluation. J Am Dent Assoc 1972;84:382–90.
  • 28. International Organization for Standardization. Technical report 7405. Dentistry – preclinical evaluation of biocompatibility of medical devices used in dentistry – test methods for dental materials. Genève: The Institution, 1997:22.
  • 29. Niedernhofer LJ, Daniels JS, Rouzer CA, Greene RE, Marnett LJ. Malondialdehyde, a product of lipid peroxidation, is mutagenic in human cells. J Biol Chem 2003;278:31426–33.
  • 30. Esterbauer H, Wäg G, Puhl H. Lipid peroxidation and its role in atherosclerosis. Br Med Bull 1993;49:566–76.
  • 31. Bir LS, Demir S, Rota S, Köseoğlu M. Increased serum malondialdehyde levels in chronic stage of ischemic stroke. Tohoku J Exp Med 2006;208:33–9.
  • 32. Agarwal R. Chronic kidney disease is associated with oxidative stress independent of hypertension. Clin Nephrol 2004;61: 377–83.
  • 33. Koulaouzidou EA, Economides N, Beltes P, Geromichalos G, Papazisis K. In vitro evaluation of the cytotoxicity of ProRoot MTA and MTA Angelus. J Oral Sci 2008;50:397–402.
  • 34. De Deus G, Ximenes R, Gurgel-Filho ED, Plotkowski MC, Coutinho-Filho T. Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells. Int Endod J 2005;38: 604–9.
  • 35. Bin CV, Valera MC, Camargo SE, Rabelo SB, Silva GO, Balducci I, et al. Cytotoxicity and genotoxicity of root canal sealers based on mineral trioxide aggregate. J Endod 2012;38:495–500.
  • 36. Silva EJ, Rosa TP, Herrera DR, Jacinto RC, Gomes BP, Zaia AA. Evaluation of cytotoxicity and physicochemical properties of calcium silicate-based endodontic sealer MTA Fillapex. J Endod 2013;39:274–7.
  • 37. Tavares CO, Böttcher DE, Assmann E, Kopper PM, de Figueiredo JA, Grecca FS, et al. Tissue reactions to a new mineral trioxide aggregate-containing endodontic sealer. J Endod 2013;39: 653–7.
  • 38. Gomes-Filho JE, de Faria MD, Bernabé PF, Nery MJ, Otoboni-Filho JA, Dezan-Júnior E, et al. Mineral trioxide aggregate but not light-cure mineral trioxide aggregate stimulated mineralization. J Endod 2008;34:62–5.
  • 39. Mori GG, Teixeira LM, de Oliveira DL, Jacomini LM, da Silva SR. Biocompatibility evaluation of biodentine in subcutaneous tissue of rats. J Endod 2014;40:1485–8.
  • 40. Khalil WA, Eid NF. Biocompatibility of BioAggregate and mineral trioxide aggregate on the liver and kidney. Int Endod J 2013;46:730–7.
  • 41. Scelza MZ, Linhares AB, da Silva LE, Granjeiro JM, Alves GG. A multiparametric assay to compare the cytotoxicity of endodontic sealers with primary human osteoblasts. Int Endod J 2012;45:12–8.
  • 42. Bidar M, Naghavi N, Mohtasham N, Sheik-Nezami M, Fallahrastegar A, Afkhami F, et al. Mineral trioxide aggregate and portland cement for direct pulp capping in dog: a histopathological evaluation. J Dent Res Dent Clin Dent Prospects 2014;8:134–40.
  • 43. Iguchi Y, Kosugi S, Nishikawa H, Lin Z, Minabe Y, Toda S. Repeated exposure of adult rats to transient oxidative stress induces various long-lasting alterations in cognitive and behavioral functions. PLoS One 2014;9:e114024.
APA Demirkaya k, DEMİRDÖĞEN B, ÖNCEL TORUN Z, Erdem O, TUNCA Y (2018). The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver. , 333 - 341. 10.1515/tjb-2016-0263
Chicago Demirkaya kadriye,DEMİRDÖĞEN Birsen Can,ÖNCEL TORUN Zeynep,Erdem Onur,TUNCA Yaşar Meriç The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver. (2018): 333 - 341. 10.1515/tjb-2016-0263
MLA Demirkaya kadriye,DEMİRDÖĞEN Birsen Can,ÖNCEL TORUN Zeynep,Erdem Onur,TUNCA Yaşar Meriç The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver. , 2018, ss.333 - 341. 10.1515/tjb-2016-0263
AMA Demirkaya k,DEMİRDÖĞEN B,ÖNCEL TORUN Z,Erdem O,TUNCA Y The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver. . 2018; 333 - 341. 10.1515/tjb-2016-0263
Vancouver Demirkaya k,DEMİRDÖĞEN B,ÖNCEL TORUN Z,Erdem O,TUNCA Y The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver. . 2018; 333 - 341. 10.1515/tjb-2016-0263
IEEE Demirkaya k,DEMİRDÖĞEN B,ÖNCEL TORUN Z,Erdem O,TUNCA Y "The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver." , ss.333 - 341, 2018. 10.1515/tjb-2016-0263
ISNAD Demirkaya, kadriye vd. "The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver". (2018), 333-341. https://doi.org/10.1515/tjb-2016-0263
APA Demirkaya k, DEMİRDÖĞEN B, ÖNCEL TORUN Z, Erdem O, TUNCA Y (2018). The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver. Türk Biyokimya Dergisi, 43(3), 333 - 341. 10.1515/tjb-2016-0263
Chicago Demirkaya kadriye,DEMİRDÖĞEN Birsen Can,ÖNCEL TORUN Zeynep,Erdem Onur,TUNCA Yaşar Meriç The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver. Türk Biyokimya Dergisi 43, no.3 (2018): 333 - 341. 10.1515/tjb-2016-0263
MLA Demirkaya kadriye,DEMİRDÖĞEN Birsen Can,ÖNCEL TORUN Zeynep,Erdem Onur,TUNCA Yaşar Meriç The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver. Türk Biyokimya Dergisi, vol.43, no.3, 2018, ss.333 - 341. 10.1515/tjb-2016-0263
AMA Demirkaya k,DEMİRDÖĞEN B,ÖNCEL TORUN Z,Erdem O,TUNCA Y The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver. Türk Biyokimya Dergisi. 2018; 43(3): 333 - 341. 10.1515/tjb-2016-0263
Vancouver Demirkaya k,DEMİRDÖĞEN B,ÖNCEL TORUN Z,Erdem O,TUNCA Y The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver. Türk Biyokimya Dergisi. 2018; 43(3): 333 - 341. 10.1515/tjb-2016-0263
IEEE Demirkaya k,DEMİRDÖĞEN B,ÖNCEL TORUN Z,Erdem O,TUNCA Y "The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver." Türk Biyokimya Dergisi, 43, ss.333 - 341, 2018. 10.1515/tjb-2016-0263
ISNAD Demirkaya, kadriye vd. "The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver". Türk Biyokimya Dergisi 43/3 (2018), 333-341. https://doi.org/10.1515/tjb-2016-0263
Sayfa Oluşturulma Tarihi
17-04-2024 12:12

İLETİŞİM

TÜBİTAK ULAKBİM TR Dizin

Yüzüncüyıl, İşçi Blokları Mahallesi

Muhsin Yazıcıoğlu Caddesi No:51/C

06530 Çankaya / ANKARA +90 (312) 298 92 00

trdizin@tubitak.gov.tr

TÜBİTAK ULAKBİM Ulusal Akademik Ağ ve Bilgi Merkezi Cahit Arf Bilgi Merkezi © Tüm Hakları Saklıdır. Gizlilik Politikası Kullanım Şartları