Yıl: 2018 Cilt: 43 Sayı: 4 Sayfa Aralığı: 425 - 434 Metin Dili: İngilizce DOI: 10.1515/tjb-2017-0241 İndeks Tarihi: 08-10-2019

Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey

Öz:
Objective: The objective of the present study was to investigatethe phenolic compositions, antioxidant, antimicrobialand cytotoxic activities of four Astragalus species, twoof which are endemic to Turkish flora.Methods: The total phenolic and flavonoid contents ofmethanol extracts obtained from four Astragalus specieswere detected using Folin-Ciocalteu and aluminum chloridecolorimetric assays. Their phenolic compositionswere identified by Liquid chromatography–mass spectrometry(LC-MS). The antioxidant activity was assayedwith phosphomolybdenum, 2,2-diphenyl-1-picrylhydrazylradical scavenging (DPPH), hydrogen peroxide scavenging,β-carotene bleaching activity, ferric-ion reducingpower (FRAP) and cupric ions (Cu2+) reducing antioxidantcapacity (CUPRAC) methods. Antimicrobial activities ofthe extracts were studied by agar well-diffusion assay. Thecytotoxic effects of the extracts on MCF-7 (human breastcancer cell lines) were determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) method.Results: The extracts exerted moderate antioxidant andreducing activity with low phenolic contents. The maincomponent in the extracts was determined as ferulic acid.The extracts demonstrated no antibacterial activity exceptP. aeruginosa. A. talasseus showed the highest cytotoxicactivity on MCF-7 during 48 h.Conclusion: It is believed that the results of this study willcontribute to research recently increasing for the use ofnatural antioxidant and antimicrobial compounds in manyindustrial fields such as food, pharmacy and medicine.
Anahtar Kelime:

Konular: Diş Hekimliği Toksikoloji Biyokimya ve Moleküler Biyoloji Farmakoloji ve Eczacılık

Türkiye’de doğal yayılış gösteren dört Astragalus türünün antioksidan ve antimikrobiyal aktiviteleri

Öz:
Amaç: Bu çalışmanın amacı Türkiye florasına ait, 2’si endemik olmak üzere dört Astragalus türünün fenolik madde içeriği, antioksidan, antimikrobiyal ve sitotoksik aktivitelerini araştırmaktır. Yöntem: Dört farklı Astragalus türünden elde edilen metanollü ekstrelerin toplam fenolik ve flavonoid madde miktarları Folin-Ciocalteu ve alüminyum klorid yöntemleri kullanılarak belirlenmiştir. Ekstrelerin fenolik bileşenleri Sıvı Kromatografisi- Kütle Spektrometresi kullanılarak belirlenmiştir. Antioksidan aktivite fosfomolibdenyum, 2,2-difenil-1-pikrilhidrazil radikal süpürücü (DPPH), hidrojen peroksit süpürücü, β-karoten beyazlatma aktivitesi, demir iyon indirgeyici güç (FRAP), indirgeyici güç ve bakır iyon (Cu2+) indirgeyici antioksidan kapasite (CUPRAC) yöntemleri kullanılarak belirlenmiştir. Ekstrelerin antimikrobiyal aktiviteleri agar difüzyon yöntemi ile çalışılmıştır. Sitotoksik aktiviteleri ise MTT yöntemi ile MCF-7 (insan meme kanser hücre hattı) kullanılarak belirlenmiştir. Bulgular: Ekstreler düşük fenolik içerikleri ile orta seviyede antioksidan ve indirgeyici aktivite göstermişlerdir. Ekstrelerdeki ana bileşen ferulik asittir. Ekstreler P. aeruginosa hariç antibakteriyal aktivite göstermemiştir. A. talasseus MCF-7 üzerinde 48 saatlik uygulamada en yüksek sitotoksik aktiviteyi göstermiştir. Sonuç: Sonuç olarak, çalışma sonuçlarının gıda, eczacılık ve tıp gibi birçok endüstriyel alanda doğal antioksidan ve antimikrobiyal bileşiklerin kullanımına yönelik son zamanlarda artan araştırmalara yardımcı olacağına inanılmaktadır.
Anahtar Kelime:

Konular: Diş Hekimliği Toksikoloji Biyokimya ve Moleküler Biyoloji Farmakoloji ve Eczacılık
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • 1. Block G, Patterson B, Subar A. Fruit, vegetables, and cancer prevention: a review of the epidemiological evidence. Nutr Cancer Int J 1992;18:1–29.
  • 2. Ness AR, Powles JW. Fruit and vegetables, and cardiovascular disease: a review. Int J Epidemiol 1997;26:1–13.
  • 3. Karadeniz F, Burdurlu HS, Koca N, Soyer Y. Antioxidant activity of selected fruits and vegetables grown in Turkey. Turk J Agric For 2005;29:297–303.
  • 4. Dung NT, Kim JM, Kang SC. Chemical composition, antimicrobial and antioxidant activities of the essential oil and the ethanol extract of Cleistocalyx operculatus (Roxb.) Merr and Perry buds. Food Chem Toxicol 2008;46:3632–9.
  • 5. Kumaran A, Karunakaran RJ. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT Food Sci Technol 2007;40:344–52.
  • 6. Hossain MA, Rahman SM. Total phenolics, flavonoids and antioxidant activity of tropical fruit pineapple. Food Res Int 2011;44:672–6.
  • 7. Davis PH. Flora of Turkey and The East Aegean Islands. Edinburgh: Edinburgh University Press, 1970:3, 49–168.
  • 8. Güner A, Özhatay N, Ekim T, Başer KH. Flora of Turkey and The East Aegean Islands, vol 11. Edinburgh: Edinburgh University Press, 2000:79–88.
  • 9. Ekici M, Aytaç Z, Akan M, Pınar M. A new species Astragalus L. (section: Onobrychoidei DC.: Fabaceae) from Turkey. Bot J Linn Soc 2008;157:741–7.
  • 10. Calış I, Dönmez AA, Perrone A, Pizza C, Piacente S. Cycloartane glycosides from Astragalus campylosema Boiss. ssp. campylosema. Phytochemistry 2008;69:2634–8.
  • 11. Gülcemal D, Alankuş-Calışkan O, Perrone A, Ozgokçe F, Piacente S, Bedir E. Cycloartane glycosides from Astragalus aureus. Phytochemistry 2011;72:761–8.
  • 12. Gülcemal D, Masullo M, Bedir E, Festa M, Karayıldırım T, Alankuş-Çalışkan Ö, et al. Triterpene glycosides from Astragalus angustifolius. Planta Med 2012;78:720–9.
  • 13. Polat E, Alankuş-Çalıskan Ö, Perrone A, Piacente S, Bedir E. Cycloartanetype glycosides from Astragalus amblolepis. Phytochemistry 2009;70:628–34.
  • 14. Polat E, Bedir E, Perrone A, Piacente S, Alankuş-Çalışkan Ö. Triterpenoid saponins from Astragalus wiedemannianus. Phytochemistry 2010;71:658–62.
  • 15. Jun MY, Kim EH, Lim JJ, Kim SH, Kim SH, Lim JD, et al. Variation of phenolic compounds contents in cultivated Astragalus membranaceus. Korean J Med Crop Sci 2012;20:447–53.
  • 16. Benchadi W, Haba H, Lavaud C, Harakat D, Benkhaled M. Secondary metabolites of Astragalus cruciatus Link. and their chemotaxonomic significance. Rec Nat Prod 2013;7:105–13.
  • 17. Lim DH, Choi DB, Choi OY, Cho KA, Kim R, Choi HS, et al. Effect of Astragalus sinicus L. seed extract on antioxidant activity. J Ind Eng Chem 2011;17:510–6.
  • 18. Chen J, Li Y, Yang LQ, Li YZ, Nan ZB, Gao K. Biological activities of flavonoids from pathogenic-infected Astragalus adsurgens. Food Chem 2012;131:546–51.
  • 19. Teyeb H, Zanina N, Neffati M, Douki W, Najjar MF. Cytotoxic and antibacterial activities of leaf extracts of Astragalus gombiformis Pomel (Fabaceae) growing wild in Tunisia. Turk J Biol 2012;36:53–8.
  • 20. Bedir E, Pugh N, Calıs I, Pasco DS, Khan IA. Immunostimulatory effects of cycloartane-type triterpene glycosides from Astragalus species. Biol Pharm Bull 2000;23:834–7.
  • 21. Uysal İ. Observations on the morphology, anatomy and ecology of endemic species Astragalus trojanus Stev. Erciyes Univ J Inst Sci Technol 1997;13:54–66.
  • 22. Albayrak S, Aksoy A, Sagdic O, Hamzaoglu E. Compositions, antioxidant and antimicrobial activities of Helichrysum (Asteraceae) species collected from Turkey. Food Chem 2010;119:114–22.
  • 23. Singleton VL, Rossi JA Jr. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965;16:144–58.
  • 24. Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol 2006;5:1142–5.
  • 25. Prieto P, Pineda M, Aguilar M. Spectrofotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999;269:337–41.
  • 26. Lee SK, Mbwambo ZH, Chung HS, Luyengi L, Games EJ, Metha RG. Evaluation of the antioxidant potential of natural products. Comb Chem High Throughput Screen 1998;1:35–46.
  • 27. Cao L, Si JY, Liu Y, Sun H, Jin W, Li Z, et al. Essential oil composition, antimicrobial and antioxidant properties of Moslachinensis Maxim. Food Chem 2009;115:801–5.
  • 28. Apak R, Güçlü K, Özyurek M, Karademir SE, Ercağ E. The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. Int J Food Sci Nutr 2006;57:292–304.
  • 29. Tuberoso CI, Montoro P, Piacente S, Corona G, Deiana M, Dessi MA, et al. Flavonoid characterization and antioxidant activity of hydroalcoholic extracts from Achillea ligustica All. J Pharm Biomed Anal 2009;50:440–8.
  • 30. Peksel A, Arisan-Atac İ, Yanardag R. Evaluation of antioxidant and antiacetylcholinesterase activities of the extracts of Pistacia atlantica desf. Leaves. J Food Biochem 2010;34:451–76.
  • 31. Aadil KR, Barapatre A, Sahu S, Jha H, Tiwary BN. Free radical scavenging activity and reducing power of Acacia nilotica wood lignin. Int J Biol Macromol 2014;67:220–7.
  • 32. Li M, Xu Y, Yang W, Li J, Xu X, Zhang X, et al. In vitro synergistic anti-oxidant activities of solvent-extracted fractions from Astragalus membranaceus and Glycyrrhiza uralensis. Food Sci Technol 2011;44:1745–51.
  • 33. SPSS, SPSS Version 10.0. SPSS Inc, Chicago, Illinois, 2001.
  • 34. Zarena AS, Manohar B, Udaya-Sankar K. Optimization of supercritical carbon dioxide extraction of xanthones from mangosteen pericarp by response surface methodology. Food Bioprocess Technol 2012;5:1181–8.
  • 35. Gülçin İ, Mshvildadze V, Gepdiremen A, Elias R. Antioxidant activity of a triterpenoid glycoside isolated from the berries of Hedera colchica:3-o-(β-d-glucopyranosyl)-hederagenin. Phytother Res 2006;20:130–4.
  • 36. Asgarpanah J, Motamed SM, Farzaneh A, Ghanizadeh B, Tomraee S. Antioxidant activity and total phenolic and flavonoid content of Astragalus squarrosus Bunge. Afr J Biotechnol 2011;10:19176–80.
  • 37. Adıgüzel A, Sökmen M, Özkan H, Ağar G, Güllüce M, Şahin F. In vitro antimicrobial and antioxidant activities of methanol and hexane extract of Astragalus species growing in the eastern Anatolia region of Turkey. Turk J Biol 2009;33:65–71.
  • 38. Pistelli L, Bertoli A, Leporia E, Morellia I, Panizzi L. Antimicrobial and antifungal activity of crude extracts and isolated saponins from Astragalus errucosus. Fitoterapia 2002;73:336–9.
  • 39. Türker AU, Yıldırım AB. Evaluation of antibacterial and antitumor activities of some Turkish endemic plants. Trop J Pharm Res 2013;12:1003–10.
  • 40. Albayrak S, Aksoy A, Albayrak S, Sagdıc O. In vitro antioxidant and antimicrobial activity of some Lamiaceae species. Iran J Sci Technol 2013;A1:1–9.
  • 41. Ionkova I, Momekov G, Proksch P. Effects of cycloartane saponins from hairy roots of Astragalus membranaceus Bge., on human tumor cell targets. Fitoterapia 2010;81:447–51.
APA ALBAYRAK S, Kaya O (2018). Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey. , 425 - 434. 10.1515/tjb-2017-0241
Chicago ALBAYRAK SEVİL,Kaya Onur Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey. (2018): 425 - 434. 10.1515/tjb-2017-0241
MLA ALBAYRAK SEVİL,Kaya Onur Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey. , 2018, ss.425 - 434. 10.1515/tjb-2017-0241
AMA ALBAYRAK S,Kaya O Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey. . 2018; 425 - 434. 10.1515/tjb-2017-0241
Vancouver ALBAYRAK S,Kaya O Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey. . 2018; 425 - 434. 10.1515/tjb-2017-0241
IEEE ALBAYRAK S,Kaya O "Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey." , ss.425 - 434, 2018. 10.1515/tjb-2017-0241
ISNAD ALBAYRAK, SEVİL - Kaya, Onur. "Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey". (2018), 425-434. https://doi.org/10.1515/tjb-2017-0241
APA ALBAYRAK S, Kaya O (2018). Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey. Türk Biyokimya Dergisi, 43(4), 425 - 434. 10.1515/tjb-2017-0241
Chicago ALBAYRAK SEVİL,Kaya Onur Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey. Türk Biyokimya Dergisi 43, no.4 (2018): 425 - 434. 10.1515/tjb-2017-0241
MLA ALBAYRAK SEVİL,Kaya Onur Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey. Türk Biyokimya Dergisi, vol.43, no.4, 2018, ss.425 - 434. 10.1515/tjb-2017-0241
AMA ALBAYRAK S,Kaya O Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey. Türk Biyokimya Dergisi. 2018; 43(4): 425 - 434. 10.1515/tjb-2017-0241
Vancouver ALBAYRAK S,Kaya O Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey. Türk Biyokimya Dergisi. 2018; 43(4): 425 - 434. 10.1515/tjb-2017-0241
IEEE ALBAYRAK S,Kaya O "Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey." Türk Biyokimya Dergisi, 43, ss.425 - 434, 2018. 10.1515/tjb-2017-0241
ISNAD ALBAYRAK, SEVİL - Kaya, Onur. "Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey". Türk Biyokimya Dergisi 43/4 (2018), 425-434. https://doi.org/10.1515/tjb-2017-0241