Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen

ACAR ŞAHİN, Aydan; Aslim, Belma; ALAN, SENOL; TAN, Sema; PINAR, Nur Münevver

doi:10.1515/tjb-2017-0260

Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen

Aydan ACAR ŞAHİN, (Department of Biology, Faculty of Sciences, Experimental Palynology Lab, Ankara University, Ankara, Turkey)

Belma ASLIM, (Department of Biology, Faculty of Sciences, Gazi University, Ankara, Turkey)

Sema TAN, (Department of Biology, Faculty of Sciences, Kırıkkale University, Ankara, Turkey)

Şenol ALAN, (Department of Biology, Faculty of Science and Art, Bülent Ecevit University, Zonguldak, Turkey)

Nur Münevver PINAR (Department of Biology, Faculty of Sciences, Ankara University, Ankara, Turkey)

Türk Biyokimya Dergisi

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Yıl: 2018 Cilt: 43 Sayı: 4 Sayfa Aralığı: 435 - 446 Metin Dili: İngilizce DOI: 10.1515/tjb-2017-0260 İndeks Tarihi: 08-10-2019

Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen

Öz:

Objective: Cupressaceae pollen has commonly beenreported to be an important aeroallergen and causal factorof spring, autumn and winter pollinosis in many countries.The aim of this study was to compare of the structureand allergenic protein content of Cupressus arizonicaGreene., Cupressus sempervirens L. and Juniperus oxycedrusL. pollen in detail and contribute to Cupressaceaepollen allergen diagnosis and therapy studies in Turkey.Methods: The pollen structure were examined by LM andSEM. Pollen protein content was investigated by Bradfordprotein assay, sodium dodecyl sulfate polyacrylamide gelelectrophoresis (SDS-PAGE), Western blot analysis andtwo-dimensional polyacrylamide gel electrophoresis (2DEPAGE), respectively. Pectate lyase (PL) enzyme activitieswere compared. Immunoblotting was carried out by usingextracts of the three taxa pollen collected from Turkey.Results: All three taxa was found very similar in termsof pollen morphology however, intine thickness wasprominently different. Cupressus arizonica pollen extractsshowed the lowest PL activity. Five sera specific IgE of allallergic subjects showed reaction with only C. arizonicapollen extracts.Conclusions: As a conclusion, the pollen structure, proteinfunction or protein structure and isoforms of allergenscould affects allergenic properties of the pollen. Thisstudy also may help to improve the Cupressaceae pollenallergen diagnosis and therapy.

Anahtar Kelime:

Konular: Diş Hekimliği Toksikoloji Biyokimya ve Moleküler Biyoloji Farmakoloji ve Eczacılık

Bazı Cupressaceae polenlerinde polen yapısı, alerjik protein içeriği ve pektat liyaz enzim aktivitesi bakımından farklılıklar

Öz:

Amaç: Cupressaceae polenlerinin birçok ülkede ilkbahar, sonbahar ve kış polinizasyon dönemleri için önemli aeroalerjen faktör olduğu sıklıkla bildirilmiştir. Bu çalışmanın amacı, Cupressus arizonica Greene, Cupressus sempervirens L. ve Juniperus oxycedrus L. polenlerin yapısını ve alerjenik protein içeriğini karşılaştırmak ve Türkiye‘de Cupressaceae polen alerjenlerinin tanısına ve alerji tedavisine katkıda bulunmaktır. Yöntemler: Polen yapısı LM ve SEM ile incelendi. Polen proteini içeriği, sırasıyla, Bradford protein testi, SDSPAGE, Western blot analizi ve 2DE PAGE ile araştırıldı. Pektat liyaz enzim aktiviteleri karşılaştırıldı. Immunoblot analizleri çalışılan üç taksona ait polen özütleri kullanılarak gerçekleştirildi. Bulgular: Her üç taksonun polen morfolojisi bakımından birbirine çok benzer olduğu ancak intinin kalınlığının taksonlar arasında belirgin olarak farklı olduğu bulundu. Cupressus arizonica polen özütlerinde en düşük pektat liyaz aktivitesi görülürken, hastaların beş tanesinde serum spesifik IgE, sadece C. arizonica polen ekstraktlarıyla reaksiyon gösterdi. Sonuç: Polen yapısı, protein fonksiyonu veya protein yapısı ve allerjenlerin izoformları polenlerin alerjenitelerini etkileyebilir. Bu çalışma ayrıca, Cupressaceae polen alerjisi tanısı ve tedavisine katkı sağlayabilir.

Anahtar Kelime:

Konular: Diş Hekimliği Toksikoloji Biyokimya ve Moleküler Biyoloji Farmakoloji ve Eczacılık

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ACAR ŞAHİN A, Aslim B, TAN S, ALAN S, PINAR N (2018). Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen. , 435 - 446. 10.1515/tjb-2017-0260
Chicago	ACAR ŞAHİN Aydan,Aslim Belma,TAN Sema,ALAN SENOL,PINAR Nur Münevver Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen. (2018): 435 - 446. 10.1515/tjb-2017-0260
MLA	ACAR ŞAHİN Aydan,Aslim Belma,TAN Sema,ALAN SENOL,PINAR Nur Münevver Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen. , 2018, ss.435 - 446. 10.1515/tjb-2017-0260
AMA	ACAR ŞAHİN A,Aslim B,TAN S,ALAN S,PINAR N Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen. . 2018; 435 - 446. 10.1515/tjb-2017-0260
Vancouver	ACAR ŞAHİN A,Aslim B,TAN S,ALAN S,PINAR N Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen. . 2018; 435 - 446. 10.1515/tjb-2017-0260
IEEE	ACAR ŞAHİN A,Aslim B,TAN S,ALAN S,PINAR N "Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen." , ss.435 - 446, 2018. 10.1515/tjb-2017-0260
ISNAD	ACAR ŞAHİN, Aydan vd. "Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen". (2018), 435-446. https://doi.org/10.1515/tjb-2017-0260

APA	ACAR ŞAHİN A, Aslim B, TAN S, ALAN S, PINAR N (2018). Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen. Türk Biyokimya Dergisi, 43(4), 435 - 446. 10.1515/tjb-2017-0260
Chicago	ACAR ŞAHİN Aydan,Aslim Belma,TAN Sema,ALAN SENOL,PINAR Nur Münevver Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen. Türk Biyokimya Dergisi 43, no.4 (2018): 435 - 446. 10.1515/tjb-2017-0260
MLA	ACAR ŞAHİN Aydan,Aslim Belma,TAN Sema,ALAN SENOL,PINAR Nur Münevver Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen. Türk Biyokimya Dergisi, vol.43, no.4, 2018, ss.435 - 446. 10.1515/tjb-2017-0260
AMA	ACAR ŞAHİN A,Aslim B,TAN S,ALAN S,PINAR N Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen. Türk Biyokimya Dergisi. 2018; 43(4): 435 - 446. 10.1515/tjb-2017-0260
Vancouver	ACAR ŞAHİN A,Aslim B,TAN S,ALAN S,PINAR N Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen. Türk Biyokimya Dergisi. 2018; 43(4): 435 - 446. 10.1515/tjb-2017-0260
IEEE	ACAR ŞAHİN A,Aslim B,TAN S,ALAN S,PINAR N "Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen." Türk Biyokimya Dergisi, 43, ss.435 - 446, 2018. 10.1515/tjb-2017-0260
ISNAD	ACAR ŞAHİN, Aydan vd. "Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen". Türk Biyokimya Dergisi 43/4 (2018), 435-446. https://doi.org/10.1515/tjb-2017-0260