TY  - JOUR
TI  - A validated method without derivatization for the determination of Letrozole by high performance liquid chromatography-fluorimetric method for pharmaceutical preparation
AB  - A high performance liquid chromatography method has been developed for determination of letrozole in tablets. The methodhas developed in reverse phase column with acetonitrile-50.0 mM phosphoric acid solution, pH:7 (50:50, v/v) and 0.7 mL/minflow rate by using a fluorimetric detector. Letrozole has natural fluorescence properties, and there is no need for derivatizationfor this molecule. The detector was set at 256, 585 nm for excitation and emission wavelength respectively. The methodwas validated in accuracy, precision, specificity, linearity and system suitability parameters are studied. The linear range wasfound 50-700 ng/mL. The limit of detection and quantification for letrozole was found to be 14-50 ng/mL respectively. Therecovery of drug is found to be 98.8%. The developed method has been validated and successfully applied to tablet analysis ofdrug substance. This method is simple, reproducible and can be used safely for routine analysis of letrozole in tablets.
AU  - UNAL, Durisehvar Ozer
AU  - KAPLAN, Cem
DO  - 10.5152.IstanbulJPharm.2018.397094
PY  - 2018
JO  - Istanbul Journal of Pharmacy
VL  - 48
IS  - 2
SN  - 2548-0731
SP  - 38
EP  - 42
DB  - TRDizin
UR  - http://search/yayin/detay/310675
ER  -