Houses can form spaces in various scales, which are consequently capable of affecting human behaviour and communal organizations. The paper aims to examine the existence and quality of relations between the behavioural inclinations of the user and the spatial syntax of the house. Space syntax is a theory and a mathematical based method that generates different spatial arrangements, in order to define the structural environment. The essential concept of syntactic approach assumes that the interior and exterior forms of spaces are shaped according to certain cultural considerations and these forms also affect social relations. In order to support the hypothesis, which indicates that the relations between the user 's spatial behaviours and the syntactic properties of the space affects to the house preferences, there were some criteria determined through a two staged case study performed in Alaşehir Satellite City, Istanbul. These critera are the sociodemographic characteristics of the users, life styles of the users and the syntactic characteristics of the house. The results of the case study provide data about the socio demographic background of the users, their life styles and spatial preference, living room usage and furniture grouping, which were substantially converted to numerical database in order to be analysed by "Georgia Institute of Technology" licensed "Spatialist" software. In this paper it is exposed that the houses reflect and demonstrate the relationship between parameters such the social status, life style and the spatial preferences of the occupiers. On the other hand, the occupiers also adjust to the possibilities or restrictions provided by the houses.