TY  - JOUR
TI  - Screening of Xylanase and Glucose Isomerase Producing Bacteria Isolated from Hot Springs in Turkey
AB  - The aim of this study was screening of xylanase and glucose isomerase producing thermophilic bacteriaisolated from some hot springs located in Aegean Region of Turkey. Total sixty eight thermophilic isolates(Anoxybacillus, Brevibacillus, Geobacillus, Aneurinibacillus, Thermus, Paenibacillus and Proteobacter)were collected previously from these fields and identified based on 16S rDNA gene sequences. Isolateswere screened by plate assay for determining the xylanase and glucose isomerase production abilitiesseperately in order to find new strains for industrial processes. After an incubation period of two days forxylanase and 5-6 days for glucose isomerase at 50-60 °C, positive isolates were determined. Enzymeproducing isolates were confirmed by spectrophotometric measurements with crude enzyme extracts,birchwood xylan and glucose were used as substrates. Most of the isolates (fifty nine) were positive forxylan degradation while only sixteen of them were positive for glucose isomerase activity. Fourteen of theisolates showed both xylanase and glucose isomerase activity. None of the isolates belong to the generaPaenibacillus, Aneurinibacillus, and Proteobacter were glucose isomerase positive, although the glucoseisomerase activity of Geobacillus isolates were notably high. Both xylanase and glucose isomeraseactivities were observed at 50-60 °C which is suitable for biotechnological applications.
AU  - BELDUZ, Ali Osman
AU  - TOKGÖZ, Müslüm
AU  - ÇOLAK, Dilşat Nigar
AU  - İNAN BEKTAŞ, Kadriye
AU  - CANAKCI, Sabriye
DO  - 10.16984/saufenbilder.356720
PY  - 2018
JO  - Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi
VL  - 22
IS  - 6
SN  - 1301-4048
SP  - 1804
EP  - 1811
DB  - TRDizin
UR  - http://search/yayin/detay/311643
ER  -