Yıl: 2018 Cilt: 33 Sayı: 3 Sayfa Aralığı: 226 - 231 Metin Dili: İngilizce DOI: 10.7161/omuanajas.407998 İndeks Tarihi: 25-11-2019

Effects of different growth regulators on regeneration of Turkish upland rice

Öz:
Rice is a staple crop grown in the world especially in the Asian region. The rice faces with differentbiotics and abiotic stress factors, especially drought stress. Turkish upland rice is one of the special ricetypes grown on drought stress condition, having a large potential value. Our aim was to analyse theeffects of different concentrations of BAP+NAA and KIN+2,4-D combination on rice grown in vitrocondition. For this purpose, mature seeds of Turkish upland rice were selected as starting material fordirect/indirect regeneration response in serial treatment. The best medium for induction of shootregeneration was MS 1 supplemented with 1.5 mg L -1 BAP and 0.5 NAA mg L -1 . The results showedthat the maximum callus formation (65.54 %) was obtained on MS medium containing 2.4-D (2.0 mg L -1 ) within 21 days from nodes. With this study, we have developed a plant tissue culture for Turkishupland rice, suggesting positive methods for rice biotechnology.
Anahtar Kelime:

Konular: Ziraat Mühendisliği

Farklı büyüme düzenleyicilerin Türk kır çeltiği rejenerasyonuna etkisi

Öz:
Çeltik bitkisi, tüm Dünya’da ve özellikle Asya kıtasında yetişen temel bir üründür. Çeltik, kuraklık stresi gibi farklı abiyotik ve biyotik stres faktörleri ile karşı karşıyadır. Türk kır çeltiği, kuraklık stres koşullarında yetişen ve büyük bir potansiyele sahip olan üstün çeltik türlerinden biridir. Bu çalışmanın amacı, BAP + NAA ve KIN + 2.4-D kombinasyonunun farklı konsantrasyonlardaki in vitro koşullarda yetiştirilen çeltik üzerindeki etkilerini incelemektir. Bu amaçla, başlangıç materyali olarak olgun çeltik tohumları kullanılmış olup direkt ve indirekt rejenerasyon sonuçları kaydedilmiştir. Gövde rejenerasyonu indüksiyonu için en iyi ortam, 1.5 mg L -1 BAP ve 0.5 NAA mg L -1 ile desteklenmiş MS 1 olmuştur. Sonuç olarak, gövde nodların 21 gün içinde 2.4-D (2.0 mg L -1 ) içeren MS ortamı üzerinde maksimum kallus oluşumunun (% 65.54) elde edildiğini kaydedilmiştir. Bu çalışma ile Türk kır çeltiği için doku kültürü optimizasyonu geliştirirmiştir ve çeltik biyoteknolojisi açısından pozitif metotlar sunulmuştur.
Anahtar Kelime:

Konular: Ziraat Mühendisliği
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Ali, S., Xue, Q., Zhang, X., 2004. Assessment of various factors involved in the tissue culture system in rice. Rice Sci. 11:345–349.
  • Bouman, B.A.M., Yang, X., Wang, H., Wang, Z., Zhao, J., Chen, B., 2006. Performance of aerobic rice cultivars under irrigated conditions in North China. Field Crops Research. 9, 53-65.
  • https://doi.org/10.1016/j.fcr.2005.08.015 Din Mohd, A.R.J., Iliyas, A.F., Wagiran, A., Abd Samad, A., Rahmat, Z., Sarmidi, M.R., 2016. Improvement of efficient in vitro regeneration potential of mature callus induced from Malaysian upland rice seed (Oryza sativa cv. Panderas). Saudi Journal of Biological Sciences. 23(1), pp. 69–77. http://doi.org/10.1016/j.sjbs.2015.10.022
  • Geng, P.P., La, H.G., Wang, H.Q., Stevens E.J.C., 2008. Effect of sorbitol concentration on regeneration of embryogenic callus in upland rice varieties (Oryza sativa L.). Plant Cell Tissue Organ Cult., 92:, 303–313. https://doi.org/10.1007/s11240-007-9321-5
  • Horuz, A., Korkmaz, A., 2014. Çeltikte (Oryza sativa L.) Tuz stresinin azaltılmasında silisyumlu gübrelemenin etkisi. Tarım Bilimleri Dergisi, 20: 215-229.
  • Jahan, N., Norshila, F., Javed, M.A., Samiullah, K., Siti, Z., 2015. Effects of ferrous toxicity on seedling traits and Ion Distribution pattern in Upland and Low Land Rice under hydroponic conditions. Jurnal Teknologi, eISSN 2180– 3722 |DOI: 10.11113/jt.v78.7258.
  • Jahan, N., Javed, M.A., Manan, F.A., Khan, S., Samad A.A., Zaidi, M.I.0., 2017. Comparative study of physiological and growth parameters for sodium and iron toxicities in upland and lowland cultivars of Oryza sativa L. Pak. J. Bot., 49(SI): 249-254.
  • Karakütük, S., 2017. Türkiye’de yetiştirilen kır çeltik çeşitlerinin in vitro koşullarda doku kültürü parametrelerinin ve kurakliğa toleranslarinin belirlenmesi. Yüksek Lisans Tezi. Ondokuz Mayıs Üniversitesi. Samsun. S. 50-55.
  • Karthikeyan A., Pandian S.T.K, Ramesh, M., 2009. High frequency plant regeneration from embryogenic callus of a popular indica rice (Oryza sativa L.). Phys. Mol. Biol. Plants, 15: 371–375. https://doi.org/10.1007/s12298-009- 0042-6.
  • Kaya, Y., Marakli, S., Gozikirmizi, N., Mohamed, E., Javed, MA., Huyop, F., 2013. Herbicide tolerance genes derived from bacteria. The J. Anim. Plant Sci., 23(1): 85–91.
  • Kaya, Y., Kuyumcu, G., Karakütük, S., Arvas Y.E., 2017. Kır çeltik bitkisi. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 27 (1):151-156. doi: 10.29133/yyutbd.305114
  • Kurt, O., Aydın, E., Seyis, F., 2008. Farklı somatik explantların çeltikte (Oryza sativa L. cv. Taipei-309) kallus ve bitkicik oluşumuna etkisi. Biyoloji Bilimler Araştırma Dergisi, 1(1):1-3.
  • Kurt, O., 2015. Hücre ve doku kültürü. Bitki ıslahı ders kitabı, No: 43 (5. Basım). Ondokuz Mayıs Üniversitesi, Ziraat Fakültesi. Samsun.
  • Kurt, S., 2016. Çeltik (Oryza sativa L.)’te somatik dokulardan kallus oluşumu ve bitki elde etme Olanakları,. Yüksek Lisans Tezi. Ondokuz Mayıs Üniversitesi, Samsun. S.60- 63.
  • Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiologia Plantarum, 15 (3): 473-497
  • Ramesh, M., Murugiah, V., Gupta AK., 2009. Efficient in vitro plant regeneration via leaf base segments of indica rice, Ind. J. Exp. Biol., 47: 68–74
  • Rueb, S., Leneman, M., Schilperoor, R., Hensgens, A., 1994. Efficient plant regeneration through somatic embryogenesis from callus induced on mature rice embryos (Oryza sativa L.). Plant Cell Tissue and Organ Culture, 36: 259-264. https://doi.org/10.1007/BF00037729
  • Sah, S. K., Kaur, A., Singh, J., (2014). High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake. J Rice Res 2:125. doi: 10.4172/jrr.1000125
  • Sahoo, K.K., Tripathi, A.K., Pareek, A., Sopory, S.K., Singla- Pareek, S.L., 2011. An improved protocol for efficient transformation and regeneration of diverse indica rice cultivars. Plant Methods, 7: 49-59. DOI: 10.1186/1746- 4811-7-49
  • Shahsavari, E., 2010. Evaluation and optimizations of media on the tissue culture system of upland rice. Int. J. Agric. Biol., 12: 537–540. Doi.org/10–177/MMI/2010/12–4– 537–540.
  • Shahsavari, E., 2011. Impact of tryptophan and glutamine on tissue culture of upland rice. Plant Soil Environ., 57: 7– 10.
  • Shahsavari, E., Maheran, AA., Siti, Nor, Akmar, A., Hanafi, MM., 2010. The effect of plant growth regulators on optimization of tissue culture system in Malaysian upland rice. Afr. J. Biotechnol, 9: 2089–2094. DOI: 10.5897/AJB10.1925.
  • Shekar, S.R.S., Singh, B., 2016. Optimization of regeneration using differential growth regulators in indica rice cultivars. 3 Biotech. 6(19). pp. 2-7. ISSN: 2190-572X (Print) 2190-5738 (Online).
  • Yang, C., Yang, L., Yang, Y., Ouyang, Z., 2004. Rice root growth and nutrient uptake as influenced by organic manure in continuously and alternately flooded paddy soils. Agricultural Water Management, 70: 67–81.
  • Zhao, W., Zheng, S., Ling, H., 2011. An regeneration system and Agrobacterium-mediated transformation of Chinese upland rice cultivar Handao297. Plant Cell Tissue Organ Cult., 106: 475–483.
  • Wang, LG., Lin, DZ., Wang, JC., 2011. Tissue culture system for different hybrid of Indica Rice. Journal of Northeast Agricultural University, 18(2): 13-17. https://doi.org/10.1016/S1006-8104(12)60003-8
APA KAYA Y, KARAKÜTÜK S (2018). Effects of different growth regulators on regeneration of Turkish upland rice. , 226 - 231. 10.7161/omuanajas.407998
Chicago KAYA YILMAZ,KARAKÜTÜK Selin Effects of different growth regulators on regeneration of Turkish upland rice. (2018): 226 - 231. 10.7161/omuanajas.407998
MLA KAYA YILMAZ,KARAKÜTÜK Selin Effects of different growth regulators on regeneration of Turkish upland rice. , 2018, ss.226 - 231. 10.7161/omuanajas.407998
AMA KAYA Y,KARAKÜTÜK S Effects of different growth regulators on regeneration of Turkish upland rice. . 2018; 226 - 231. 10.7161/omuanajas.407998
Vancouver KAYA Y,KARAKÜTÜK S Effects of different growth regulators on regeneration of Turkish upland rice. . 2018; 226 - 231. 10.7161/omuanajas.407998
IEEE KAYA Y,KARAKÜTÜK S "Effects of different growth regulators on regeneration of Turkish upland rice." , ss.226 - 231, 2018. 10.7161/omuanajas.407998
ISNAD KAYA, YILMAZ - KARAKÜTÜK, Selin. "Effects of different growth regulators on regeneration of Turkish upland rice". (2018), 226-231. https://doi.org/10.7161/omuanajas.407998
APA KAYA Y, KARAKÜTÜK S (2018). Effects of different growth regulators on regeneration of Turkish upland rice. Anadolu Tarım Bilimleri Dergisi, 33(3), 226 - 231. 10.7161/omuanajas.407998
Chicago KAYA YILMAZ,KARAKÜTÜK Selin Effects of different growth regulators on regeneration of Turkish upland rice. Anadolu Tarım Bilimleri Dergisi 33, no.3 (2018): 226 - 231. 10.7161/omuanajas.407998
MLA KAYA YILMAZ,KARAKÜTÜK Selin Effects of different growth regulators on regeneration of Turkish upland rice. Anadolu Tarım Bilimleri Dergisi, vol.33, no.3, 2018, ss.226 - 231. 10.7161/omuanajas.407998
AMA KAYA Y,KARAKÜTÜK S Effects of different growth regulators on regeneration of Turkish upland rice. Anadolu Tarım Bilimleri Dergisi. 2018; 33(3): 226 - 231. 10.7161/omuanajas.407998
Vancouver KAYA Y,KARAKÜTÜK S Effects of different growth regulators on regeneration of Turkish upland rice. Anadolu Tarım Bilimleri Dergisi. 2018; 33(3): 226 - 231. 10.7161/omuanajas.407998
IEEE KAYA Y,KARAKÜTÜK S "Effects of different growth regulators on regeneration of Turkish upland rice." Anadolu Tarım Bilimleri Dergisi, 33, ss.226 - 231, 2018. 10.7161/omuanajas.407998
ISNAD KAYA, YILMAZ - KARAKÜTÜK, Selin. "Effects of different growth regulators on regeneration of Turkish upland rice". Anadolu Tarım Bilimleri Dergisi 33/3 (2018), 226-231. https://doi.org/10.7161/omuanajas.407998