Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi

SARIÇAM İNCE, Seyyide; Mustak, Hamit Kaan

Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi

Seyyide SARIÇAM, (Ankara Üniversitesi)

Hamit Kaan MÜŞTAK (Ankara Üniversitesi)

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2018 Cilt: 65 Sayı: 4 Sayfa Aralığı: 407 - 411 Metin Dili: Türkçe İndeks Tarihi: 11-12-2019

Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi

Öz:

Bu çalışmada broyler tavuk kümeslerinin altlık, toz, çevresel, kemirici kapanları, yem ve su örneklerinden izole edilen 20 adet Salmonella Infantis suşunun multilokus dizi tiplendirme (MLST) ile tiplendirilmesi amaçlandı. Sekans tipleri (ST) ve serotipler arasındaki evrimsel ilişkiyi göstermek amacıyla yapılan filogenetik analizde, 6 farklı serotip çalışmaya dahil edildi. Her bir suşa ait 7 housekeeping gen üzerinden gerçekleştirilen MLST analizi sonucunda tüm S. Infantis suşları ST32 bulundu ve Türkiye’de S. Infantis için baskın sekans tipi olabileceği sonucuna varıldı. S. Enteritidis ST11, S. Typhimurium ST19, S. Hadar ST33, S. Agona ST13, S. Mbandaka ST413 ve S. Coeln ST2015 bulundu. S. Agona, S. Mbandaka ve S. Coeln serotipleri Türkiye’de ilk kez bu çalışmada, MLST ile tiplendirildi. İlk kez bu çalışma ile ST2015 için serotip bilgisi S. Coeln olarak MLST veritabanına girildi.

Anahtar Kelime:

Konular: Biyoloji

Phylogenetic Analysis of Salmonella Infantis Strains from Poultry by Multilocus Sequence Typing

Öz:

In this study, it was aimed to evaluate the 20 Salmonella Infantis strains isolated from broiler chicken hutches (base, dust, environment, rodent traps, feed and water samples) by Multilocus Sequence Typing (MLST). Six different serovars were used to exhibit the evolutionary relationship between different sequence types (ST) and serotypes on phylogenetic tree. All S. Infantis strains were found as ST32 as a result of MLST analysis performed on 7 housekeeping genes belonging to each subspecies and ST32 was thought to be the dominant sequence type for S. Infantis in Turkey. The other serotypes; S. Enteritidis, S. Typhimurium, S. Hadar, S. Agona, S. Mbandaka and S. Coeln was found to be ST11, ST19, ST33, ST13, ST413 and ST2015, respectively. In this study, S. Agona, S. Mbandaka and S. Coeln serotypes were typed with the MLST for the first time in Turkey. Serotype information of ST2015 was registered to the MLST database for S. Coeln serotype.

Anahtar Kelime:

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Acar S, Bulut E, Durul B ve ark. (2017): Phenotyping and genetic characterization of Salmonella enterica isolates from Turkey revealing arise of different features specific to geography. Int J Food Micro, 241, 98-107. 2. Achtman M, Wain J, Weil FX ve ark. (2012): Multilocus Sequence Typing as a replacement for serotyping in Salmonella enterica. Plos Pathog, 8, 15-16.
3. Almeida F, Pitondo-Sılva A, Aparecida OM ve ark. (2013): Molecular epidemiology and virulence markers of Salmonella Infantis isolated over 25 years in São Paulo State, Brazil. Infect Genet Evol, 19, 145-151.
4. Andrei A, Zervos MJ (2006): The application of molecular techniques to the study of hospital infection. Arch Pathol Lab Med, 130, 662-668. 5. Antunes P, Mourão J, Machado J ve ark. (2011): First description of qnrS1 IncN plasmid in a ST11 Salmonella Enteritidis clinical isolate from Portugal. Diagn Micr Infec Dis, 69, 463–465.
6. Bado I, García-Fulgueiras V, Cordeiro NF ve ark. (2012): First Human Isolate of Salmonella enterica Serotype Enteritidis Harboring blaCTX-M-14 in South America. Antimicrob Agents Ch, 56, 2132-2134.
7. Cai Y, Tao J, Jiao Y ve ark. (2016): Phenotypic characteristics and genotypic correlation between Salmonella isolates from a slaughterhouse and retail markets in Yangzhou, China. Int J Food Microbiol, 222, 5664.
8. Carattoli A, Filetici E, Villa L ve ark. (2002): Antibiotic Resistance Genes and Salmonella Genomic Island 1 in Salmonella enterica Serovar Typhimurium Isolated in Italy. Antimicrob Agents Ch, 46, 2821–2828.
9. David M, Aanensen DM, Spratt BG (2005): The Multilocus Sequence Typing Network: mlst.net. Nucl Acids Res, 33, 772-733.
10. Dione M (2010): Epidemiology of Non-Typhoidal Salmonella (Nts) in Humans and Animals in the Gambia and Senegal. Tropıcultura, 28, 253-256.
11. Durul B, Acar S, Bulut E ve ark. (2015): Subtyping of Salmonella food isolates suggests the geographic clustering of serotype Telaviv. Foodborne Pathog Dis, 12, 1-4.
12. EFSA (2012): Scientific Opinion of the Panel on Biological Hazards on a request from the European Commission on special measures to reduce the risk for consumers through Salmonella in table eggs-e.g. cooling of table eggs. EFSA J, 957, 1-19.
13. Feasey NA, Dougan G, Kingsley RA ve ark. (2012): Invasive non-typhoidal Salmonella disease: an emerging and neglected tropical disease in Africa. Lancet, 379, 2489–2499.
14. Felsenstein J (1985): Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39, 783-791.
15. Ghaderi R, Tadayon K, Khaki P ve ark. (2015): Iranian clonal population of Salmonella enterica serovar Enteritidis, characterized by multi-locus sequence typing (MLST) Method. I J M, 7, 251-259.
16. Grimont PAD, Weill FX. (2007). Antigenic formulae of the Salmonella serovars. Institut Pasteur, 9, 15-166.
17. Günel E, Kılıc G, Bulut E ve ark. (2015): Salmonella surveillance on fresh produce in retail in Turkey. Int J Food Micro, 199, 72-77.
18. Ho YN, Chou MY, Tsai HC ve ark. (2017): Empirical testing of modified Salmonella MLST in aquatic environmental samples by in silico analysis. Sci Total Environ, 581-582, 378–380.
19. Hoszowski A, Zajac M, Lalak A ve ark. (2016): Fifteen years of successful spread of Salmonella enterica serovar Mbandaka clone ST413 in Poland and its public health consequences. Ann Agr Env Med, 23, 237–241. 20. Imperial College (2007): Multi Locus Sequence Typing. http://www.mlst.net/. (5 Nisan 2017)
21. ISO 6579 (2002): General guidance on methods for the detection of Salmonella. International organisation for standardization. Microbiology, 4, 1-19.
22. Kidgell C, Reichard U, Wain J ve ark. (2002): Salmonella typhi, the causative agent of typhoid fever, is approximately 50,000 years old. Infect Genet Evol, 2, 39–40.
23. Kotetishvili M, Stine O, Kreger A, Morris Jg, Sulakvelidze A (2002): Multilocus sequence typing for characterization of clinical and environmental salmonella strains. J Clin Microbiol, 40, 1626-1635.
24. Kumar S, Stecher G, Tamura K (2016): MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol, 33, 1870-1874.
25. Li YC, Pan ZM, Kang XL ve ark. (2014): Prevalence, Characteristics, and Antimicrobial Resistance Patterns of Salmonella in Retail Pork in Jiangsu Province, Eastern China. J Food Protect, 77, 236–245.
26. Lublin A, Maler I, Mechani S ve ark. (2015): Survival of Salmonella enterica serovar Infantis on and within stored table eggs. J Food Protect, 78, 287-292.
27. Maiden MCJ, Bygraves A, Feil E ve ark. (1998): Multilocus sequence typing: A portable approach to the identification of clones within populations of pathogenic microorganisms. Proc Natl Acad Sci, 95, 3140–3145.
28. Miller T, Prager R, Rabsch W ve ark. (2010): Epidemiological relationship between Salmonella Infantis isolates of human and broiler origin. Lohmann Information, 45, 27.
29. Noda T, Murakami K, Asai T ve ark. (2011): Multi-locus sequence typing of Salmonella enterica subsp. enterica serovar Enteritidis strains in Japan between 1973 and 2004. Acta Vet Scand, 53,1.
30. Ranjbar R, Elhaghi P, Shokoohizadeh L (2016): Multilocus Sequence Typing of the Clinical Isolates of Salmonella Enterica Serovar Typhimurium in Tehran Hospitals. IJMS, 1-6.
31. Saitou N, Nei M (1987): The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol, 4, 406-425.
32. Tamura K (1992): Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Mol Biol Evol, 9, 678-687.
33. Taylor JW, Fisher MC (2003): Fungal Multilocus Sequence Typing-it’s not just For bacteria. Curr Op In Microbiol, 6, 351-356.
34. Torpdahl MIA, Skov MN, Sandvang D ve ark. (2005): Genotypic characterization of Salmonella by Multilocus Sequence Typing, Pulsed-Field Gel Electropresis and Amplified Fragment Length Polymorphism. J Microbiol Meth, 63, 173-184.
35. University Of Oxford (2010): Multi Locus Sequence Typing. http://pubmlst.org/. (5 Nisan 2017) 36. Wattiau P, Boland C, Bertrand S (2011): Methodologies for Salmonella enterica subsp. enterica subtyping: gold standards and alternatives. Appl Environ Microbiol, 7881-7882.

APA	SARIÇAM İNCE S, Mustak H (2018). Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi. , 407 - 411.
Chicago	SARIÇAM İNCE Seyyide,Mustak Hamit Kaan Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi. (2018): 407 - 411.
MLA	SARIÇAM İNCE Seyyide,Mustak Hamit Kaan Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi. , 2018, ss.407 - 411.
AMA	SARIÇAM İNCE S,Mustak H Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi. . 2018; 407 - 411.
Vancouver	SARIÇAM İNCE S,Mustak H Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi. . 2018; 407 - 411.
IEEE	SARIÇAM İNCE S,Mustak H "Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi." , ss.407 - 411, 2018.
ISNAD	SARIÇAM İNCE, Seyyide - Mustak, Hamit Kaan. "Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi". (2018), 407-411.

APA	SARIÇAM İNCE S, Mustak H (2018). Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 65(4), 407 - 411.
Chicago	SARIÇAM İNCE Seyyide,Mustak Hamit Kaan Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi. Ankara Üniversitesi Veteriner Fakültesi Dergisi 65, no.4 (2018): 407 - 411.
MLA	SARIÇAM İNCE Seyyide,Mustak Hamit Kaan Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.65, no.4, 2018, ss.407 - 411.
AMA	SARIÇAM İNCE S,Mustak H Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2018; 65(4): 407 - 411.
Vancouver	SARIÇAM İNCE S,Mustak H Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2018; 65(4): 407 - 411.
IEEE	SARIÇAM İNCE S,Mustak H "Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 65, ss.407 - 411, 2018.
ISNAD	SARIÇAM İNCE, Seyyide - Mustak, Hamit Kaan. "Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi". Ankara Üniversitesi Veteriner Fakültesi Dergisi 65/4 (2018), 407-411.