Yıl: 2003 Cilt: 27 Sayı: 5 Sayfa Aralığı: 601 - 608 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase

Öz:
Glucose-6-phosphate dehydrogenase (G6PD) enzyme was purified from sheep lenses, and the in vitro effects of dexamethasone on the enzyme's activity were investigated. Sheep lens glucose-6-phosphate dehydrogenase was purified 10,000-fold by using ammonium sulphate fractionation and 2',5'-ADP-Sepharose 4B affinity gel chromatography for a yield of 83.8% and a specific activity of 7.6 EU/mg protein. Enzyme activity was determined by Beutler's method. Dexamethasone strongly inhibited the enzyme under in vitro conditions. The I50 value of the dexamethasone was 3.05 mM.
Anahtar Kelime:

Konular: Mühendislik, Kimya
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA BEYDEMİR Ş, KULAÇOĞLU D, Ciftci M, KÜFREVİOĞLU Ö (2003). The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. , 601 - 608.
Chicago BEYDEMİR Şükrü,KULAÇOĞLU Destan Nil,Ciftci Mehmet,KÜFREVİOĞLU Ö. İrfan The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. (2003): 601 - 608.
MLA BEYDEMİR Şükrü,KULAÇOĞLU Destan Nil,Ciftci Mehmet,KÜFREVİOĞLU Ö. İrfan The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. , 2003, ss.601 - 608.
AMA BEYDEMİR Ş,KULAÇOĞLU D,Ciftci M,KÜFREVİOĞLU Ö The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. . 2003; 601 - 608.
Vancouver BEYDEMİR Ş,KULAÇOĞLU D,Ciftci M,KÜFREVİOĞLU Ö The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. . 2003; 601 - 608.
IEEE BEYDEMİR Ş,KULAÇOĞLU D,Ciftci M,KÜFREVİOĞLU Ö "The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase." , ss.601 - 608, 2003.
ISNAD BEYDEMİR, Şükrü vd. "The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase". (2003), 601-608.
APA BEYDEMİR Ş, KULAÇOĞLU D, Ciftci M, KÜFREVİOĞLU Ö (2003). The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. Turkish Journal of Chemistry, 27(5), 601 - 608.
Chicago BEYDEMİR Şükrü,KULAÇOĞLU Destan Nil,Ciftci Mehmet,KÜFREVİOĞLU Ö. İrfan The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. Turkish Journal of Chemistry 27, no.5 (2003): 601 - 608.
MLA BEYDEMİR Şükrü,KULAÇOĞLU Destan Nil,Ciftci Mehmet,KÜFREVİOĞLU Ö. İrfan The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. Turkish Journal of Chemistry, vol.27, no.5, 2003, ss.601 - 608.
AMA BEYDEMİR Ş,KULAÇOĞLU D,Ciftci M,KÜFREVİOĞLU Ö The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. Turkish Journal of Chemistry. 2003; 27(5): 601 - 608.
Vancouver BEYDEMİR Ş,KULAÇOĞLU D,Ciftci M,KÜFREVİOĞLU Ö The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. Turkish Journal of Chemistry. 2003; 27(5): 601 - 608.
IEEE BEYDEMİR Ş,KULAÇOĞLU D,Ciftci M,KÜFREVİOĞLU Ö "The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase." Turkish Journal of Chemistry, 27, ss.601 - 608, 2003.
ISNAD BEYDEMİR, Şükrü vd. "The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase". Turkish Journal of Chemistry 27/5 (2003), 601-608.