The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase

KULAÇOĞLU, Destan Nil; KÜFREVİOĞLU, Ö. İrfan; BEYDEMİR, Şükrü; Ciftci, Mehmet

The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase

Şükrü BEYDEMİR, (Atatürk Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü, Erzurum, Türkiye)

Destan Nil KULAÇOĞLU, (Girilmedi)

Mehmet ÇİFTÇİ, (Girilmedi)

Ö. İrfan KÜFREVİOĞLU (Girilmedi)

Turkish Journal of Chemistry

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Yıl: 2003 Cilt: 27 Sayı: 5 Sayfa Aralığı: 601 - 608 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase

Öz:

Glucose-6-phosphate dehydrogenase (G6PD) enzyme was purified from sheep lenses, and the in vitro effects of dexamethasone on the enzyme's activity were investigated. Sheep lens glucose-6-phosphate dehydrogenase was purified 10,000-fold by using ammonium sulphate fractionation and 2',5'-ADP-Sepharose 4B affinity gel chromatography for a yield of 83.8% and a specific activity of 7.6 EU/mg protein. Enzyme activity was determined by Beutler's method. Dexamethasone strongly inhibited the enzyme under in vitro conditions. The I50 value of the dexamethasone was 3.05 mM.

Anahtar Kelime:

Konular: Mühendislik, Kimya

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	BEYDEMİR Ş, KULAÇOĞLU D, Ciftci M, KÜFREVİOĞLU Ö (2003). The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. , 601 - 608.
Chicago	BEYDEMİR Şükrü,KULAÇOĞLU Destan Nil,Ciftci Mehmet,KÜFREVİOĞLU Ö. İrfan The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. (2003): 601 - 608.
MLA	BEYDEMİR Şükrü,KULAÇOĞLU Destan Nil,Ciftci Mehmet,KÜFREVİOĞLU Ö. İrfan The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. , 2003, ss.601 - 608.
AMA	BEYDEMİR Ş,KULAÇOĞLU D,Ciftci M,KÜFREVİOĞLU Ö The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. . 2003; 601 - 608.
Vancouver	BEYDEMİR Ş,KULAÇOĞLU D,Ciftci M,KÜFREVİOĞLU Ö The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. . 2003; 601 - 608.
IEEE	BEYDEMİR Ş,KULAÇOĞLU D,Ciftci M,KÜFREVİOĞLU Ö "The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase." , ss.601 - 608, 2003.
ISNAD	BEYDEMİR, Şükrü vd. "The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase". (2003), 601-608.

APA	BEYDEMİR Ş, KULAÇOĞLU D, Ciftci M, KÜFREVİOĞLU Ö (2003). The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. Turkish Journal of Chemistry, 27(5), 601 - 608.
Chicago	BEYDEMİR Şükrü,KULAÇOĞLU Destan Nil,Ciftci Mehmet,KÜFREVİOĞLU Ö. İrfan The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. Turkish Journal of Chemistry 27, no.5 (2003): 601 - 608.
MLA	BEYDEMİR Şükrü,KULAÇOĞLU Destan Nil,Ciftci Mehmet,KÜFREVİOĞLU Ö. İrfan The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. Turkish Journal of Chemistry, vol.27, no.5, 2003, ss.601 - 608.
AMA	BEYDEMİR Ş,KULAÇOĞLU D,Ciftci M,KÜFREVİOĞLU Ö The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. Turkish Journal of Chemistry. 2003; 27(5): 601 - 608.
Vancouver	BEYDEMİR Ş,KULAÇOĞLU D,Ciftci M,KÜFREVİOĞLU Ö The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. Turkish Journal of Chemistry. 2003; 27(5): 601 - 608.
IEEE	BEYDEMİR Ş,KULAÇOĞLU D,Ciftci M,KÜFREVİOĞLU Ö "The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase." Turkish Journal of Chemistry, 27, ss.601 - 608, 2003.
ISNAD	BEYDEMİR, Şükrü vd. "The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase". Turkish Journal of Chemistry 27/5 (2003), 601-608.