Objective: Conscience is a mental process that affects attitudes and behaviors in private and professional life and a multidimensional concept with philosophical, ethical and moral aspects. The conscientious disturbance experienced by nurses in professional life may lead to negative feelings such as guilt, sadness, hopelessness and weakness, and may cause them to experience professional burnout in the future. Method: This study is a methodological research, and the study consisted of 163 nurses. Data were collected using a personal information form and the Perceptions of Conscience Questionnaire developed by Dahlqvist et al. After the assessment of the language adaptation and content validity of the scale, data were analyzed in terms of inter-rater agreement for content validity. Factor analysis was used for construct validity. The reliability of the instrument was evaluated using item total correlation, Cronbach’s alpha coefficients and test-retest analyzes. Results: There was an inter-rater agreement in terms of the content validity evaluation scores of the questionnaire (Kendall’s W=0.114; p=0.101 > 0.05). The item-total score correlation coefficients (r) of the scale were between 0.090 and 0.697, and there was a positively significant relationship between them (p<0.001). In the reliability analysis, Cronbach’s alpha coefficient was 0.84 and test-retest correlation analysis r=0.97 was determined. Conclusion: The statistical data showed that, after removing three items from the original version of the 16-item questionnaire, the 13-item Turkish version of the questionnaire is suitable for use in studies to be conducted with nurses in Turkey.