Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey
Yıl: 2019 Cilt: 59 Sayı: 1 Sayfa Aralığı: 7 - 14 Metin Dili: İngilizce DOI: 10.16955/bitkorb.508890 İndeks Tarihi: 13-01-2020
Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey
Öz: Apple, pear and apricot trees showing phytoplasma associated symptoms from Ankara and Isparta provinces were sampled and investigated to verify the presence of phytoplasma-associated diseases. Totally 31 samples were tested with phytoplasma universal and group specific primers and the PCR products were restricted using Tru1I, RsaI and SspI endonucleases. Different RFLP profiles were obtained and selected samples were directly sequenced. The apple samples were found infected with 16SrX-A (‘Candidatus Phytoplasma mali’), while the majority of the pear samples were infected with 16SrX-A and 16SrX-C subgroup phytoplasmas in mixed infection. The 16SrX-B (‘Candidatus Phytoplasma prunorum’), 16SrX-C (‘Candidatus Phytoplasma pyri’) and mixed infection of 16SrX-A/16SrX-C and 16SrX-C/16SrI (aster yellows) were detected in the apricot samples. In this study the 16SrI phytoplasmas in apricot and the mixed phytoplasma infections in pear and apricot trees were detected in Turkey for the first time.
Anahtar Kelime: Türkiye’de yumuşak ve sert çekirdekli meyvelerde ‘ Candidatus Phytoplasma’ türlerinin moleküler çeşitliliği
Öz: Ankara ve Isparta illerinden fitoplazma enfeksiyonu benzeri belirti gösteren elma, armut ve kayısı ağaçlarından fitoplazma varlığınınsaptanmasınayönelikörneklemeyapılmıştır.Toplamda 31 örnek universal ve grup spesifik fitoplazmaprimerleriilePCR’atabitutulmuşveeldeedilenPCRürünleri Tr u 1 I, Rsa I and Ssp I endonükleazları ile kesilmiştir. Elma örnekleri 16SrX-A (‘ CandidatusPhytoplasma mali’)ile enfekteli bulunurken armut örneklerinin çoğunluğunda16SrX-Ave16SrX-Caltgruplarıylakarışıkenfeksiyonsaptanmıştır.Kayısıörneklerinde16SrX-B(‘ Candidatus Phytoplasmaprunorum’),16SrX-C(‘ Candidatus Phytoplasmapyri’)ve16SrX-A/16SrX-Cve16SrX- C/16SrI (aster yellows) alt gruplarının karışık enfeksiyonusaptanmıştır.Buçalışmailekayısıda16SrI,armuttavekayısıda karışık fitoplazma enfeksiyonları Türkiye’de ilk kez saptanmıştır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Derleme Erişim Türü: Erişime Açık
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| APA | CANİK OREL D, PALTRINIERI S, ERTUNÇ F, BERTACCINI A (2019). Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey. , 7 - 14. 10.16955/bitkorb.508890 |
| Chicago | CANİK OREL DİDEM,PALTRINIERI Samanta,ERTUNÇ Filiz,BERTACCINI Assunta Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey. (2019): 7 - 14. 10.16955/bitkorb.508890 |
| MLA | CANİK OREL DİDEM,PALTRINIERI Samanta,ERTUNÇ Filiz,BERTACCINI Assunta Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey. , 2019, ss.7 - 14. 10.16955/bitkorb.508890 |
| AMA | CANİK OREL D,PALTRINIERI S,ERTUNÇ F,BERTACCINI A Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey. . 2019; 7 - 14. 10.16955/bitkorb.508890 |
| Vancouver | CANİK OREL D,PALTRINIERI S,ERTUNÇ F,BERTACCINI A Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey. . 2019; 7 - 14. 10.16955/bitkorb.508890 |
| IEEE | CANİK OREL D,PALTRINIERI S,ERTUNÇ F,BERTACCINI A "Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey." , ss.7 - 14, 2019. 10.16955/bitkorb.508890 |
| ISNAD | CANİK OREL, DİDEM vd. "Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey". (2019), 7-14. https://doi.org/10.16955/bitkorb.508890 |
| APA | CANİK OREL D, PALTRINIERI S, ERTUNÇ F, BERTACCINI A (2019). Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey. Bitki Koruma Bülteni, 59(1), 7 - 14. 10.16955/bitkorb.508890 |
| Chicago | CANİK OREL DİDEM,PALTRINIERI Samanta,ERTUNÇ Filiz,BERTACCINI Assunta Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey. Bitki Koruma Bülteni 59, no.1 (2019): 7 - 14. 10.16955/bitkorb.508890 |
| MLA | CANİK OREL DİDEM,PALTRINIERI Samanta,ERTUNÇ Filiz,BERTACCINI Assunta Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey. Bitki Koruma Bülteni, vol.59, no.1, 2019, ss.7 - 14. 10.16955/bitkorb.508890 |
| AMA | CANİK OREL D,PALTRINIERI S,ERTUNÇ F,BERTACCINI A Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey. Bitki Koruma Bülteni. 2019; 59(1): 7 - 14. 10.16955/bitkorb.508890 |
| Vancouver | CANİK OREL D,PALTRINIERI S,ERTUNÇ F,BERTACCINI A Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey. Bitki Koruma Bülteni. 2019; 59(1): 7 - 14. 10.16955/bitkorb.508890 |
| IEEE | CANİK OREL D,PALTRINIERI S,ERTUNÇ F,BERTACCINI A "Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey." Bitki Koruma Bülteni, 59, ss.7 - 14, 2019. 10.16955/bitkorb.508890 |
| ISNAD | CANİK OREL, DİDEM vd. "Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey". Bitki Koruma Bülteni 59/1 (2019), 7-14. https://doi.org/10.16955/bitkorb.508890 |
