TY  - JOUR
TI  - Amylase Production of Bacillus subtilis Isolated from Soil by SmF Method
AB  - In our study, the isolation, identification and optimization of Bacillus subtilis from soil samples were performedand the ability to produce α-amylase of industrial importance was investigated. Bacteria identified as B. subtilis by 16SrRNA analysis, morphological and biochemical tests. Maximum growth was determined at 32 hour, 37°C and pH 7.0.Enzyme optimization was performed after determination of bacterial amylase in starchy medium. Optimum conditionsfor amylase production were determined as 37°C, pH 7.0 and 48 h. When the effect of metals on enzyme activity wasexamined, it was determined that Cu2+ increased enzyme activity, Zn2+, Hg2+ and Fe2+ were partially inhibited theenzyme activity, and Ca2+ was showed close activity to the control. It was observed that carbon sources had inhibitoryeffect on bacterial growth and enzyme production.
AU  - ORTAKAYA, Veysi
AU  - AGÜLOĞLU FİNCAN, Sema
DO  - 10.5505/biodicon.2019.70288
PY  - 2019
JO  - Biological Diversity and Conservation
VL  - 12
IS  - 3
SN  - 1308-5301
SP  - 57
EP  - 64
DB  - TRDizin
UR  - http://search/yayin/detay/318400
ER  -