TY  - JOUR
TI  - Investigation of Breast Cancer Cells and Phospholipid Cell Membrane Interactions
AB  - Objective: Circulating tumor cells have an important role in the pathogenesis of metastasis. Metastasis occursthrough few steps including arrival of circulating tumor cells to distant tissue and organs, their adherence to thetarget tissue, and then formation of a new tumor. To understand the mechanism of this process it is necessary toinvestigate the interaction of cancer cells with other molecules and cells of the target tissue, and most importantlyinteraction with lipids forming the cellular membrane.Methods: To better understand the process of cancer cell adhesion onto lipid membranes and the ionic interactionsthat are involved in cell adherence, surfaces functionalized with tethered bilayer lipid membrane (tBLM) were utilized in this work as an experimental platform. Either lipid surfaces functionalized with cationic POEPC: PC or anionic POPS: PC fwere examined to observe the ionic interaction of charged phospholipid membrane and MDA-MB231 breast cancer cells.Results: Adhesions of MDA-MB-231 breast cancer cells and NIH-3T3 mouse fibroblast cells to positively chargedPOEPC: PC lipid surfaces,and their dissemination was observed during examinations using Surface PlasmonResonance (SPR) method. The results were further confirmed with cell viability and proliferation studies that showscationic POEPC: PC lipid surfaces were able to facilitate and increase the cell adhesion.Conclusion: These results reveal the cationic phospholipid structures favour the enhanced cancer cell adhesion.
AU  - ARSLAN YILDIZ, AHU
DO  - 10.5222/terh.2019.26918
PY  - 2019
JO  - İzmir Tepecik Eğitim Hastanesi Dergisi
VL  - 29
IS  - 1
SN  - 1305-7073
SP  - 21
EP  - 27
DB  - TRDizin
UR  - http://search/yayin/detay/319820
ER  -