Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum

Işıl Melis ZÜMRÜT, (Yeni Yüzyil Üniversitesi, Fen Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, İstanbul, Türkiye)
Berna TUNALI, (Ondokuz Mayıs Üniversitesi, Ziraat Fakültesi, Bitki Patolojisi Anabilim Dalı, Samsun, Türkiye)
Bayram KANSU, (Ondokuz Mayıs Üniversitesi, Meslek Yüksekokulu, Bitkisel ve Hayvansal Üretim Bölümü, Samsun, Türkiye)
Gülşen UZ, (Yeni Yüzyil Üniversitesi, Fen Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, İstanbul, Türkiye)
Emre YÖRÜK, (Yeni Yüzyil Üniversitesi, Fen Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, İstanbul, Türkiye)
Fatih ÖLMEZ, (Tarla Bitkileri Merkez Araştırma Enstitüsü, Biyoteknoloji Bölümü, Ankara, Türkiye)
Ayşegül SARIKAYA (Yeni Yüzyil Üniversitesi, Fen Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, İstanbul, Türkiye)
Türk Tarım - Gıda Bilim ve Teknoloji dergisi
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Yıl: 2018 Cilt: 6 Sayı: 2 Sayfa Aralığı: 145 - 149 Metin Dili: İngilizce DOI: 10.24925/turjaf.v6i2.145-149.1423 İndeks Tarihi: 26-02-2020

Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum

Öz:
Fusarium culmorum is predominating causal agent of head blight (HB) and root rot (RR)in cereals worldwide. Since F. culmorum has a great level of genetic diversity and theparasexual stage is assumed for this phytopathogen, characterization of isolates fromdifferent regions is significant step in food safety and controlling the HB. In this study, itwas aimed to characterize totally 37 F. culmorum isolates from Turkey via sequencerelated amplified polymorphism (SRAP) marker based genotyping. MAT-1/MAT-2 typeassay was also used in order to reveal intraspecific variation in F. culmorum. MAT-1 andMAT-2 specific primer pairs for mating assays resulted in 210 and 260 bp bands,respectively. 11 of isolates were belonged to MAT-1 type whereas 19 samples were ofMAT-2. Remaining 7 samples yielded both amplicons. Totally 9 SRAP primer setsyielded amplicons from all isolates. Genetic similarity values were ranged from 39 to94.7%. Total band number was 127 and PCR product sizes were in the range of 0.1-2.5kb. Amplicon numbers for individuals were ranged from 1 to 16. According to dataobtained from current study, SRAP based genotyping is powerful tool for supporting thedata obtained from investigations including phenotypic and agro-ecologicalcharacteristics. Findings showed that SRAP-based markers could be useful in F.culmorum characterization.
Anahtar Kelime:

Konular: Ziraat Mühendisliği Gıda Bilimi ve Teknolojisi
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA ZÜMRÜT I, Tunali B, KANSU B, UZ G, yörük e, ÖLMEZ F, SARIKAYA A (2018). Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum. , 145 - 149. 10.24925/turjaf.v6i2.145-149.1423
Chicago ZÜMRÜT Işıl Melis,Tunali Berna,KANSU BAYRAM,UZ Gülşen,yörük emre,ÖLMEZ FATİH,SARIKAYA Ayşegül Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum. (2018): 145 - 149. 10.24925/turjaf.v6i2.145-149.1423
MLA ZÜMRÜT Işıl Melis,Tunali Berna,KANSU BAYRAM,UZ Gülşen,yörük emre,ÖLMEZ FATİH,SARIKAYA Ayşegül Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum. , 2018, ss.145 - 149. 10.24925/turjaf.v6i2.145-149.1423
AMA ZÜMRÜT I,Tunali B,KANSU B,UZ G,yörük e,ÖLMEZ F,SARIKAYA A Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum. . 2018; 145 - 149. 10.24925/turjaf.v6i2.145-149.1423
Vancouver ZÜMRÜT I,Tunali B,KANSU B,UZ G,yörük e,ÖLMEZ F,SARIKAYA A Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum. . 2018; 145 - 149. 10.24925/turjaf.v6i2.145-149.1423
IEEE ZÜMRÜT I,Tunali B,KANSU B,UZ G,yörük e,ÖLMEZ F,SARIKAYA A "Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum." , ss.145 - 149, 2018. 10.24925/turjaf.v6i2.145-149.1423
ISNAD ZÜMRÜT, Işıl Melis vd. "Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum". (2018), 145-149. https://doi.org/10.24925/turjaf.v6i2.145-149.1423
APA ZÜMRÜT I, Tunali B, KANSU B, UZ G, yörük e, ÖLMEZ F, SARIKAYA A (2018). Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, 6(2), 145 - 149. 10.24925/turjaf.v6i2.145-149.1423
Chicago ZÜMRÜT Işıl Melis,Tunali Berna,KANSU BAYRAM,UZ Gülşen,yörük emre,ÖLMEZ FATİH,SARIKAYA Ayşegül Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum. Türk Tarım - Gıda Bilim ve Teknoloji dergisi 6, no.2 (2018): 145 - 149. 10.24925/turjaf.v6i2.145-149.1423
MLA ZÜMRÜT Işıl Melis,Tunali Berna,KANSU BAYRAM,UZ Gülşen,yörük emre,ÖLMEZ FATİH,SARIKAYA Ayşegül Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, vol.6, no.2, 2018, ss.145 - 149. 10.24925/turjaf.v6i2.145-149.1423
AMA ZÜMRÜT I,Tunali B,KANSU B,UZ G,yörük e,ÖLMEZ F,SARIKAYA A Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum. Türk Tarım - Gıda Bilim ve Teknoloji dergisi. 2018; 6(2): 145 - 149. 10.24925/turjaf.v6i2.145-149.1423
Vancouver ZÜMRÜT I,Tunali B,KANSU B,UZ G,yörük e,ÖLMEZ F,SARIKAYA A Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum. Türk Tarım - Gıda Bilim ve Teknoloji dergisi. 2018; 6(2): 145 - 149. 10.24925/turjaf.v6i2.145-149.1423
IEEE ZÜMRÜT I,Tunali B,KANSU B,UZ G,yörük e,ÖLMEZ F,SARIKAYA A "Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum." Türk Tarım - Gıda Bilim ve Teknoloji dergisi, 6, ss.145 - 149, 2018. 10.24925/turjaf.v6i2.145-149.1423
ISNAD ZÜMRÜT, Işıl Melis vd. "Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum". Türk Tarım - Gıda Bilim ve Teknoloji dergisi 6/2 (2018), 145-149. https://doi.org/10.24925/turjaf.v6i2.145-149.1423
Sayfa Oluşturulma Tarihi
23-04-2024 10:35

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